Question 1

A northern blot differs from a Southern blot in the

Accepted Answer

A)  number of genetic sequences detected. 
B)  type of nucleic acid being isolated. 
C)  type of probe used. 
D)  size of the genetic sequences involved. 
E)  presence or absence of a nitrocellulose membrane. 
A)  number of genetic sequences detected. 
B)  type of nucleic acid being isolated. 
C)  type of probe used. 
D)  size of the genetic sequences involved. 
E)  presence or absence of a nitrocellulose membrane.

Question 2

DNA encoding a normal gene is inserted into cells from a patient with a defective form of the gene. Recombinant cells are identified and isolated, and returned to the patient's body. This is an example of

Accepted Answer

A)  gene therapy. 
B)  genetic fingerprinting. 
C)  genotyping. 
D)  genomics. 
E)  biotechnology. 
A)  gene therapy. 
B)  genetic fingerprinting. 
C)  genotyping. 
D)  genomics. 
E)  biotechnology.

Question 3

A technique using fluorescent- tagged probes to detect specific DNA sequences in their natural locations is known as (FISH/PCR/BLOTTING).

Accepted Answer

The answer of A technique using fluorescent- tagged probes to...

Question 4

Mutagens are useful in biotechnology research for

Accepted Answer

A)  producing DNA fragments for cloning. 
B)  removing undesirable traits from microbes. 
C)  producing new organisms which have beneficial traits from two or more organisms. 
D)  producing organisms with altered phenotypes. 
E)  selecting genetic mutants resistant to radioactivity. 
A)  producing DNA fragments for cloning. 
B)  removing undesirable traits from microbes. 
C)  producing new organisms which have beneficial traits from two or more organisms. 
D)  producing organisms with altered phenotypes. 
E)  selecting genetic mutants resistant to radioactivity.

Question 5

Gene therapy for human genetic diseases has not been successful yet.

Accepted Answer

A) True 
 B)False

Question 6

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Accepted Answer

A)  1000 base pairs 
B)  5000 base pairs 
C)  2500 base pairs 
D)  750 base pairs 
E)  250 base pairs 
A)  1000 base pairs 
B)  5000 base pairs 
C)  2500 base pairs 
D)  750 base pairs 
E)  250 base pairs

Question 7

A DNA (library/microarray) may be used to study the complex, changing patterns of mRNA production in an organism.

Accepted Answer

The answer of A DNA (library/microarray) may be used to...

Question 8

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Accepted Answer

A)  Bacillus thuringiensis 
B)  Deinococcus radiodurans 
C)  Pseudomonas 
D)  Salmonella 
E)  Escherichia coli 
A)  Bacillus thuringiensis 
B)  Deinococcus radiodurans 
C)  Pseudomonas 
D)  Salmonella 
E)  Escherichia coli

Question 9

Which of the following statements regarding vectors is FALSE?

Accepted Answer

A)  Cloning vectors include a "marker" to facilitate identification of cells containing them. 
B)  A useful vector contains multiple restriction sites for insertion of DNA. 
C)  Vectors are usually autonomously replicating DNA molecules. 
D)  Vectors are generally over 100,000 base pairs in size. 
E)  Cloning vectors frequently contain sequences necessary for expression of inserted sequences. 
A)  Cloning vectors include a "marker" to facilitate identification of cells containing them. 
B)  A useful vector contains multiple restriction sites for insertion of DNA. 
C)  Vectors are usually autonomously replicating DNA molecules. 
D)  Vectors are generally over 100,000 base pairs in size. 
E)  Cloning vectors frequently contain sequences necessary for expression of inserted sequences.

Question 10

Discuss some of the impacts that tools and techniques of recombinant DNA technology have had on medicine.

Accepted Answer

Human gene libraries and DNA sequencing

Question 11

Study of the genome of Deinococcus radiodurans may provide insight into preventing or correcting genetic damage resulting from (radiation/mutation).

Accepted Answer

The answer of Study of the genome of Deinococcus radiodurans...

Question 12

Which of the following would be an appropriate sequence of temperatures for PCR?

Accepted Answer

A)  94°C, 37°C, 55°C 
B)  94°C, 55°C, 37°C 
C)  94°C, 65°C, 72°C 
D)  72°C, 65°C, 94°C 
E)  65°C, 72°C, 94°C 
A)  94°C, 37°C, 55°C 
B)  94°C, 55°C, 37°C 
C)  94°C, 65°C, 72°C 
D)  72°C, 65°C, 94°C 
E)  65°C, 72°C, 94°C

Question 13

A collection of bacterial clones each of which contains a portion of the gene sequences of an organism is known as a microarray of that organism's genome.

Accepted Answer

A) True 
 B)False

Question 14

Injecting DNA into cells can be accomplished using

Accepted Answer

A)  silicon chips and nucleic acids. 
B)  compressed air and gold beads. 
C)  nitrocellulose membranes. 
D)  micropipettes. 
E)  micropipettes, or compressed air and gold beads. 
A)  silicon chips and nucleic acids. 
B)  compressed air and gold beads. 
C)  nitrocellulose membranes. 
D)  micropipettes. 
E)  micropipettes, or compressed air and gold beads.

Question 15

The natural role of restriction enzymes in bacteria is to

Accepted Answer

A)  allow transposons to move to another place in the chromosome. 
B)  protect the cell from invading phages. 
C)  make conjugation more efficient. 
D)  allow cells to accept foreign DNA. 
E)  provide the cell with new phenotypes, such as antibiotic resistance. 
A)  allow transposons to move to another place in the chromosome. 
B)  protect the cell from invading phages. 
C)  make conjugation more efficient. 
D)  allow cells to accept foreign DNA. 
E)  provide the cell with new phenotypes, such as antibiotic resistance.

Question 16

Subunit vaccines are safer than traditional vaccines because they

Accepted Answer

A)  are acellular and do not pose a risk for causing the disease. 
B)  do not pose a risk for causing the disease. 
C)  are acellular. 
D)  are acellular and can be administered in food. 
E)  are administered in food. 
A)  are acellular and do not pose a risk for causing the disease. 
B)  do not pose a risk for causing the disease. 
C)  are acellular. 
D)  are acellular and can be administered in food. 
E)  are administered in food.

Question 17

Which method of inserting DNA into cells is illustrated in this figure?

Accepted Answer

A)  electroporation 
B)  protoplast fusion 
C)  transduction 
D)  injection 
E)  a gene gun 
A)  electroporation 
B)  protoplast fusion 
C)  transduction 
D)  injection 
E)  a gene gun

Question 18

In gel electrophoresis, DNA molecules move toward the electrode because they have an overall _ charge.

Accepted Answer

A)  negative and positive; neutral 
B)  negative; positive 
C)  positive; negative 
D)  negative; negative 
E)  positive; positive 
A)  negative and positive; neutral 
B)  negative; positive 
C)  positive; negative 
D)  negative; negative 
E)  positive; positive

Question 19

Transgenic organisms

Accepted Answer

A)  contain genetically engineered microbes. 
B)  contain cells from other organisms. 
C)  are the same as clones. 
D)  have genomes that have been sequenced completely. 
E)  contain genes from other organisms. 
A)  contain genetically engineered microbes. 
B)  contain cells from other organisms. 
C)  are the same as clones. 
D)  have genomes that have been sequenced completely. 
E)  contain genes from other organisms.

Question 20

In Southern blotting, DNA molecules are immobilized on

Accepted Answer

A)  nitrocellulose membranes. 
B)  silicon chips. 
C)  paper. 
D)  gold beads. 
E)  agarose. 
A)  nitrocellulose membranes. 
B)  silicon chips. 
C)  paper. 
D)  gold beads. 
E)  agarose.

Exam 8: Recombinant Dna Technology

A northern blot differs from a Southern blot in the

DNA encoding a normal gene is inserted into cells from a patient with a defective form of the gene. Recombinant cells are identified and isolated, and returned to the patient's body. This is an example of

A technique using fluorescent- tagged probes to detect specific DNA sequences in their natural locations is known as (FISH/PCR/BLOTTING).

Mutagens are useful in biotechnology research for

Gene therapy for human genetic diseases has not been successful yet.

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

A DNA (library/microarray) may be used to study the complex, changing patterns of mRNA production in an organism.

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Which of the following statements regarding vectors is FALSE?

Discuss some of the impacts that tools and techniques of recombinant DNA technology have had on medicine.

Study of the genome of Deinococcus radiodurans may provide insight into preventing or correcting genetic damage resulting from (radiation/mutation).

Which of the following would be an appropriate sequence of temperatures for PCR?

A collection of bacterial clones each of which contains a portion of the gene sequences of an organism is known as a microarray of that organism's genome.

Injecting DNA into cells can be accomplished using

The natural role of restriction enzymes in bacteria is to

Subunit vaccines are safer than traditional vaccines because they

Which method of inserting DNA into cells is illustrated in this figure?

In gel electrophoresis, DNA molecules move toward the electrode because they have an overall _ charge.

Transgenic organisms

In Southern blotting, DNA molecules are immobilized on

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Disease, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram-Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic Dna Viruses

Pathogenic Rna Viruses

Applied and Environmental Microbiology

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