Question 1

Probes used for detecting genetic sequences are frequently composed of

Accepted Answer

A)  synthetic nucleic acids and labeled conjugates, such as fluorescent dyes. 
B)  restriction enzymes. 
C)  nitrocellulose. 
D)  agarose. 
E)  silicon chips. 
A)  synthetic nucleic acids and labeled conjugates, such as fluorescent dyes. 
B)  restriction enzymes. 
C)  nitrocellulose. 
D)  agarose. 
E)  silicon chips.

Question 2

The use of microbes to make practical products such as vaccines or hormones is called (genomics/recombination/biotechnology).

Accepted Answer

The answer of The use of microbes to make practical...

Question 3

Which of the following microbes produces a protein that kills a variety of insect pests?

Accepted Answer

A)  Pseudomonas 
B)  Plasmodium falciparum 
C)  Haemophilus influenzae 
D)  Bacillus thuringiensis 
E)  Thermus aquaticus 
A)  Pseudomonas 
B)  Plasmodium falciparum 
C)  Haemophilus influenzae 
D)  Bacillus thuringiensis 
E)  Thermus aquaticus

Question 4

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?

Accepted Answer

A)  use a microarray to detect a transcript of the gene 
B)  use a labeled synthetic probe complementary to the gene sequence 
C)  use electrophoresis to identify plasmids containing an insert of the expected size 
D)  sequence the DNA of the plasmids from each isolate 
E)  assay for activity of the gene product 
A)  use a microarray to detect a transcript of the gene 
B)  use a labeled synthetic probe complementary to the gene sequence 
C)  use electrophoresis to identify plasmids containing an insert of the expected size 
D)  sequence the DNA of the plasmids from each isolate 
E)  assay for activity of the gene product

Question 5

are used in the detection of DNA by FISH.

Accepted Answer

A)  Restriction fragments 
B)  Compressed air and gold beads 
C)  Reverse transcriptase and DNA polymerase 
D)  DNA polymerase and DNA ligase 
E)  Synthetic DNAs and fluorescent tags 
A)  Restriction fragments 
B)  Compressed air and gold beads 
C)  Reverse transcriptase and DNA polymerase 
D)  DNA polymerase and DNA ligase 
E)  Synthetic DNAs and fluorescent tags

Question 6

A set of clones representing the entire genome of an organism is known as a gene (recombinant/map/library).

Accepted Answer

The answer of A set of clones representing the entire...

Question 7

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel (electroporation/electrophoresis).

Accepted Answer

The answer of The northern blot is a technique used...

Question 8

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/primers/vectors).

Accepted Answer

The answer of Short nucleic acid molecules used to locate...

Question 9

Some of the strongest opposition to the application of recombinant DNA technology concerns

Accepted Answer

A)  DNA fingerprinting. 
B)  correcting gene defects in animals. 
C)  modification of food crops. 
D)  sequencing of the human genome. 
E)  pest control measures. 
A)  DNA fingerprinting. 
B)  correcting gene defects in animals. 
C)  modification of food crops. 
D)  sequencing of the human genome. 
E)  pest control measures.

Question 10

Compare and contrast the two major categories of restriction enzymes.

Accepted Answer

All restriction enzymes recognize specif

Question 11

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

Accepted Answer

A)  32 
B)  16 
C)  100 
D)  6 
E)  64 
A)  32 
B)  16 
C)  100 
D)  6 
E)  64

Question 12

The process of locating genes within the nucleic acid of an organism is called (genetic/library/clone) mapping.

Accepted Answer

The answer of The process of locating genes within the...

Question 13

Which of the following devices is used for PCR?

Accepted Answer

A)  a gene gun 
B)  a nucleic acid synthesis machine 
C)  a DNA sequencer 
D)  an electrophoresis chamber 
E)  a thermocycler 
A)  a gene gun 
B)  a nucleic acid synthesis machine 
C)  a DNA sequencer 
D)  an electrophoresis chamber 
E)  a thermocycler

Question 14

Vectors usually contain genetic (markers/sequences) such as antibiotic genes or fluorescent tags.

Accepted Answer

The answer of Vectors usually contain genetic (markers/sequences) such as...

Question 15

Under ideal conditions, the number of DNA molecules produced during PCR increases exponentially.

Accepted Answer

A) True 
 B)False

Exam 8: Recombinant Dna Technology

Probes used for detecting genetic sequences are frequently composed of

The use of microbes to make practical products such as vaccines or hormones is called (genomics/recombination/biotechnology).

Which of the following microbes produces a protein that kills a variety of insect pests?

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?

are used in the detection of DNA by FISH.

A set of clones representing the entire genome of an organism is known as a gene (recombinant/map/library).

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel (electroporation/electrophoresis).

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/primers/vectors).

Some of the strongest opposition to the application of recombinant DNA technology concerns

Compare and contrast the two major categories of restriction enzymes.

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

The process of locating genes within the nucleic acid of an organism is called (genetic/library/clone) mapping.

Which of the following devices is used for PCR?

Vectors usually contain genetic (markers/sequences) such as antibiotic genes or fluorescent tags.

Under ideal conditions, the number of DNA molecules produced during PCR increases exponentially.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Disease, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram-Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic Dna Viruses

Pathogenic Rna Viruses

Applied and Environmental Microbiology

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