Question 1

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following recombinant tools is NOT used in DNA fingerprinting?

Accepted Answer

A)  reverse transcription 
B)  gel electrophoresis 
C)  PCR 
D)  restriction enzyme digestion 
E)  Neither PCR nor gel electrophoresis is used. 
A)  reverse transcription 
B)  gel electrophoresis 
C)  PCR 
D)  restriction enzyme digestion 
E)  Neither PCR nor gel electrophoresis is used.

Question 3

The procedure used to identify individuals by their unique genetic sequences is known as

Accepted Answer

A)  northern analysis. 
B)  xenotransplantation. 
C)  DNA sequencing. 
D)  DNA fingerprinting. 
E)  microarray analysis. 
A)  northern analysis. 
B)  xenotransplantation. 
C)  DNA sequencing. 
D)  DNA fingerprinting. 
E)  microarray analysis.

Question 4

DNA fingerprinting produces a unique banding pattern of DNA fragments for comparison with other DNA samples.

Accepted Answer

A) True 
 B)False

Question 5

Recombinant DNA technology can be most accurately defined as the

Accepted Answer

A)  study of replication and recombination in microbes. 
B)  deliberate modification of the genome of an organism for practical purposes. 
C)  selective breeding of organisms to create new combinations of traits. 
D)  study of genetic expression in microbes. 
E)  use of microorganisms to produce useful products. 
A)  study of replication and recombination in microbes. 
B)  deliberate modification of the genome of an organism for practical purposes. 
C)  selective breeding of organisms to create new combinations of traits. 
D)  study of genetic expression in microbes. 
E)  use of microorganisms to produce useful products.

Question 6

Recent modifications of microbes have produced

Accepted Answer

A)  improved beer production. 
B)  a cure for AIDS. 
C)  microbes capable of producing crude oil. 
D)  microbes which facilitate extraction of metals from mining ores. 
E)  new emerging diseases. 
A)  improved beer production. 
B)  a cure for AIDS. 
C)  microbes capable of producing crude oil. 
D)  microbes which facilitate extraction of metals from mining ores. 
E)  new emerging diseases.

Question 7

Synthetic nucleic acids are useful as

Accepted Answer

A)  DNA probes, primers, and antisense RNAs. 
B)  DNA probes. 
C)  DNA probes and antisense RNAs. 
D)  antisense RNAs. 
E)  primers for PCR. 
A)  DNA probes, primers, and antisense RNAs. 
B)  DNA probes. 
C)  DNA probes and antisense RNAs. 
D)  antisense RNAs. 
E)  primers for PCR.

Question 8

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Accepted Answer

A)  the modification of crop plants 
B)  answering basic research questions 
C)  unforeseen impact on the environment 
D)  the modification of animals to produce pharmaceuticals for humans 
E)  screening of humans for genes that predispose them to disease 
A)  the modification of crop plants 
B)  answering basic research questions 
C)  unforeseen impact on the environment 
D)  the modification of animals to produce pharmaceuticals for humans 
E)  screening of humans for genes that predispose them to disease

Question 9

Synthesis of cDNA requires the use of

Accepted Answer

A)  restriction enzymes. 
B)  reverse transcriptase. 
C)  agarose. 
D)  DNA ligase. 
E)  fluorescent synthetic nucleotides. 
A)  restriction enzymes. 
B)  reverse transcriptase. 
C)  agarose. 
D)  DNA ligase. 
E)  fluorescent synthetic nucleotides.

Question 10

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Accepted Answer

A) True 
 B)False

Question 11

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?

Accepted Answer

A)  PCR 
B)  Southern blotting or PCR 
C)  creation of a gene library 
D)  Southern blotting 
E)  genome mapping 
A)  PCR 
B)  Southern blotting or PCR 
C)  creation of a gene library 
D)  Southern blotting 
E)  genome mapping

Question 12

The DNA double helix can be separated into single strands using

Accepted Answer

A)  NaOH. 
B)  heat. 
C)  reverse transcriptase. 
D)  either heat or NaOH. 
E)  heat, NaOH, and reverse transcriptase. 
A)  NaOH. 
B)  heat. 
C)  reverse transcriptase. 
D)  either heat or NaOH. 
E)  heat, NaOH, and reverse transcriptase.

Question 13

In the 20th century, scientists harnessed the natural metabolic reactions of bacteria to make for the first time in an industrial setting.

Accepted Answer

A)  acetone 
B)  cheese 
C)  alcohol 
D)  soy sauce 
E)  wine 
A)  acetone 
B)  cheese 
C)  alcohol 
D)  soy sauce 
E)  wine

Question 14

A microarray is assembled using

Accepted Answer

A)  gold beads and magnets. 
B)  restriction enzymes. 
C)  single- stranded DNA and silicon chips. 
D)  agarose and nitrocellulose. 
E)  reverse transcriptase and DNA polymerase. 
A)  gold beads and magnets. 
B)  restriction enzymes. 
C)  single- stranded DNA and silicon chips. 
D)  agarose and nitrocellulose. 
E)  reverse transcriptase and DNA polymerase.

Question 15

DNA fingerprinting can be used

Accepted Answer

A)  to detect unculturable organisms. 
B)  in forensic investigations. 
C)  to generate cDNA clones. 
D)  for forensics and detection of unculturable organisms. 
E)  to generate cDNA clones and libraries. 
A)  to detect unculturable organisms. 
B)  in forensic investigations. 
C)  to generate cDNA clones. 
D)  for forensics and detection of unculturable organisms. 
E)  to generate cDNA clones and libraries.

Question 16

Beta- carotene, the biochemical precursor to vitamin A, can be added to rice by using (biotechnology/cloning/recombinant) DNA technology, thereby increasing its nutritional value.

Accepted Answer

The answer of Beta- carotene, the biochemical precursor to vitamin...

Question 17

Which of the following is essential in PCR?

Accepted Answer

A)  antisense RNAs 
B)  DNA primers 
C)  DNA polymerase 
D)  both DNA primers and DNA polymerase 
E)  reverse transcriptase 
A)  antisense RNAs 
B)  DNA primers 
C)  DNA polymerase 
D)  both DNA primers and DNA polymerase 
E)  reverse transcriptase

Question 18

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Accepted Answer

The answer of Nucleic acid molecules used to deliver new...

Question 19

Synthetic nucleic acids are produced

Accepted Answer

A)  by PCR of the desired sequence. 
B)  using restriction enzymes to cut a fragment out of a plasmid containing the desired sequence. 
C)  by a series of chemical steps carried out on the lab bench. 
D)  using computer- controlled machines to assemble a single strand nucleic acid. 
E)  using a recombinant cell which produces a transcript of the desired sequence. 
A)  by PCR of the desired sequence. 
B)  using restriction enzymes to cut a fragment out of a plasmid containing the desired sequence. 
C)  by a series of chemical steps carried out on the lab bench. 
D)  using computer- controlled machines to assemble a single strand nucleic acid. 
E)  using a recombinant cell which produces a transcript of the desired sequence.

Question 20

Restriction (enzymes/proteases), first isolated from bacterial cells, cut DNA molecules at specific sites.

Accepted Answer

The answer of Restriction (enzymes/proteases), first isolated from bacterial cells,...

Exam 8: Recombinant Dna Technology

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Which of the following recombinant tools is NOT used in DNA fingerprinting?

The procedure used to identify individuals by their unique genetic sequences is known as

DNA fingerprinting produces a unique banding pattern of DNA fragments for comparison with other DNA samples.

Recombinant DNA technology can be most accurately defined as the

Recent modifications of microbes have produced

Synthetic nucleic acids are useful as

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Synthesis of cDNA requires the use of

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?

The DNA double helix can be separated into single strands using

In the 20th century, scientists harnessed the natural metabolic reactions of bacteria to make for the first time in an industrial setting.

A microarray is assembled using

DNA fingerprinting can be used

Beta- carotene, the biochemical precursor to vitamin A, can be added to rice by using (biotechnology/cloning/recombinant) DNA technology, thereby increasing its nutritional value.

Which of the following is essential in PCR?

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Synthetic nucleic acids are produced

Restriction (enzymes/proteases), first isolated from bacterial cells, cut DNA molecules at specific sites.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Disease, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram-Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic Dna Viruses

Pathogenic Rna Viruses

Applied and Environmental Microbiology

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