Question 1

Why are modern light microscopes better than the ones Leeuwenhoek used?

Accepted Answer

A)  Modern microscopes are compound instead of simple. 
B)  Modern lenses are made of prisms. 
C)  Modern microscopes have lenses with smaller numerical apertures. 
D)  Modern microscopes are compound and have fivefold better resolution. 
E)  Modern microscopes have a fivefold better resolution. 
A)  Modern microscopes are compound instead of simple. 
B)  Modern lenses are made of prisms. 
C)  Modern microscopes have lenses with smaller numerical apertures. 
D)  Modern microscopes are compound and have fivefold better resolution. 
E)  Modern microscopes have a fivefold better resolution.

Question 2

Which of the following microscopes produces the highest resolution images?

Accepted Answer

A)  scanning electron 
B)  dark- field 
C)  atomic force 
D)  differential interference 
E)  fluorescent 
A)  scanning electron 
B)  dark- field 
C)  atomic force 
D)  differential interference 
E)  fluorescent

Question 3

Which of the following are magnifying lenses?

Accepted Answer

A)  dark- field stops 
B)  oculars 
C)  condensers 
D)  objectives 
E)  both objectives and the oculars 
A)  dark- field stops 
B)  oculars 
C)  condensers 
D)  objectives 
E)  both objectives and the oculars

Question 4

Acidic dyes

Accepted Answer

A)  are used for staining negatively charged molecular structures. 
B)  are negatively charged and work best at low pH. 
C)  are lipid soluble. 
D)  work best in low pH environments. 
E)  are negatively charged. 
A)  are used for staining negatively charged molecular structures. 
B)  are negatively charged and work best at low pH. 
C)  are lipid soluble. 
D)  work best in low pH environments. 
E)  are negatively charged.

Question 5

A structure that appears in a transmission electron micrograph but is NOT actually present in the specimen is known as a(n)

Accepted Answer

A)  antigen. 
B)  mordant. 
C)  artifact. 
D)  biofilm. 
E)  refraction. 
A)  antigen. 
B)  mordant. 
C)  artifact. 
D)  biofilm. 
E)  refraction.

Question 6

What role does safranin play in the Gram stain procedure?

Accepted Answer

A)  primary stain 
B)  negative stain 
C)  decolorizing agent 
D)  mordant 
E)  counterstain 
A)  primary stain 
B)  negative stain 
C)  decolorizing agent 
D)  mordant 
E)  counterstain

Question 7

The microscope preferred for viewing living specimens is the microscope.

Accepted Answer

A)  transmission electron 
B)  bright- field 
C)  phase- contrast 
D)  scanning tunneling 
E)  scanning electron 
A)  transmission electron 
B)  bright- field 
C)  phase- contrast 
D)  scanning tunneling 
E)  scanning electron

Question 8

Which of the following is NOT associated with an electron microscope?

Accepted Answer

A)  a vacuum 
B)  magnetic fields 
C)  a fluorescent screen 
D)  a prism 
E)  an electron beam 
A)  a vacuum 
B)  magnetic fields 
C)  a fluorescent screen 
D)  a prism 
E)  an electron beam

Question 9

The three domains proposed by Carl Woese are the Archaea, the Eukarya, and the Protista.

Accepted Answer

A) True 
 B)False

Question 10

Acid- fast cells such as Mycobacterium lose the color of the primary stain in the presence of hydrochloric acid.

Accepted Answer

A) True 
 B)False

Question 11

Discuss the ways in which light rays can be manipulated to increase resolution and/or contrast.

Accepted Answer

Light rays can be manipulated to increas

Question 12

If you were trying to visualize flagella without staining, which microscope would you use?

Accepted Answer

A)  confocal 
B)  phase- contrast 
C)  dark- field 
D)  bright- field 
E)  fluorescent 
A)  confocal 
B)  phase- contrast 
C)  dark- field 
D)  bright- field 
E)  fluorescent

Question 13

A serological test that involves the clumping of antigen and antibody is the (agglutination/antigen/ELISA) test.

Accepted Answer

The answer of A serological test that involves the clumping...

Question 14

Viruses are generally measured in

Accepted Answer

A)  nanometers. 
B)  centimeters. 
C)  micrometers. 
D)  decimeters. 
E)  millimeters. 
A)  nanometers. 
B)  centimeters. 
C)  micrometers. 
D)  decimeters. 
E)  millimeters.

Question 15

Which of the following phenomena produces magnification?

Accepted Answer

A)  the refraction of radiation as it passes through a lens 
B)  the length of an objective lens 
C)  the numerical aperture of a lens 
D)  the wavelength of a radiation source 
E)  the thickness of a microscopic specimen 
A)  the refraction of radiation as it passes through a lens 
B)  the length of an objective lens 
C)  the numerical aperture of a lens 
D)  the wavelength of a radiation source 
E)  the thickness of a microscopic specimen

Question 16

The stain is one in which eosin is used.

Accepted Answer

A)  flagellar 
B)  endospore 
C)  electron microscopy 
D)  negative 
E)  acid- fast 
A)  flagellar 
B)  endospore 
C)  electron microscopy 
D)  negative 
E)  acid- fast

Question 17

One- thousandth of a meter is a

Accepted Answer

A)  nanometer (nm). 
B)  millimeter (mm). 
C)  yard. 
D)  centimeter (cm). 
E)  micrometer (µm). 
A)  nanometer (nm). 
B)  millimeter (mm). 
C)  yard. 
D)  centimeter (cm). 
E)  micrometer (µm).

Question 18

List and explain five types of techniques that can be used to identify unknown microorganisms.

Accepted Answer

There are five major types of identifica

Question 19

Which of the following is an INCORRECT pairing?

Accepted Answer

A)  simple microscope; Leeuwenhoek 
B)  magnification; refraction of radiation 
C)  electron beams; shorter wavelength 
D)  contrast; staining techniques 
E)  numerical aperture; curved glass 
A)  simple microscope; Leeuwenhoek 
B)  magnification; refraction of radiation 
C)  electron beams; shorter wavelength 
D)  contrast; staining techniques 
E)  numerical aperture; curved glass

Question 20

Compare and contrast the three domains identified by Woese: Eukarya, Bacteria, and Archaea.

Accepted Answer

With respect to cell type, organisms in

Exam 4: Microscopy, Staining, and Classification

Why are modern light microscopes better than the ones Leeuwenhoek used?

Which of the following microscopes produces the highest resolution images?

Which of the following are magnifying lenses?

Acidic dyes

A structure that appears in a transmission electron micrograph but is NOT actually present in the specimen is known as a(n)

What role does safranin play in the Gram stain procedure?

The microscope preferred for viewing living specimens is the microscope.

Which of the following is NOT associated with an electron microscope?

The three domains proposed by Carl Woese are the Archaea, the Eukarya, and the Protista.

Acid- fast cells such as Mycobacterium lose the color of the primary stain in the presence of hydrochloric acid.

Discuss the ways in which light rays can be manipulated to increase resolution and/or contrast.

If you were trying to visualize flagella without staining, which microscope would you use?

A serological test that involves the clumping of antigen and antibody is the (agglutination/antigen/ELISA) test.

Viruses are generally measured in

Which of the following phenomena produces magnification?

The stain is one in which eosin is used.

One- thousandth of a meter is a

List and explain five types of techniques that can be used to identify unknown microorganisms.

Which of the following is an INCORRECT pairing?

Compare and contrast the three domains identified by Woese: Eukarya, Bacteria, and Archaea.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant Dna Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Disease, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram-Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic Dna Viruses

Pathogenic Rna Viruses

Applied and Environmental Microbiology

Filters