Question 1

A sample from a patient is prepared using the Gomori methenamine silver stain. What type of microbe is suspected of being present?

Accepted Answer

A)  protozoal parasite 
B)  virus 
C)  fungus 
D)  parasitic worm larva 
E)  bacteria 
A)  protozoal parasite 
B)  virus 
C)  fungus 
D)  parasitic worm larva 
E)  bacteria

Question 2

Which of the following is NOT a characteristic of a genus name?

Accepted Answer

A)  It is written before the specific epithet. 
B)  It is usually an adjective. 
C)  It is always capitalized. 
D)  It is either underlined or in italics. 
E)  It is one of two names used to identify an organism. 
A)  It is written before the specific epithet. 
B)  It is usually an adjective. 
C)  It is always capitalized. 
D)  It is either underlined or in italics. 
E)  It is one of two names used to identify an organism.

Question 3

Scanning tunneling microscopes map the topography of a specimen by detecting the deflection of the probe tip.

Accepted Answer

A) True 
 B)False

Question 4

Species and strains of microbes can be distinguished from one another phenotypically using

Accepted Answer

A)  ribosomal RNA analysis 
B)  Gram- stain reactions. 
C)  G + C content. 
D)  electron microscopy. 
E)  serological tests. 
A)  ribosomal RNA analysis 
B)  Gram- stain reactions. 
C)  G + C content. 
D)  electron microscopy. 
E)  serological tests.

Question 5

In the Gram stain procedure, iodine serves as a

Accepted Answer

A)  fixative. 
B)  counterstain. 
C)  primary stain. 
D)  decolorizing agent. 
E)  mordant. 
A)  fixative. 
B)  counterstain. 
C)  primary stain. 
D)  decolorizing agent. 
E)  mordant.

Question 6

An integral part of serological testing is the use of a solution called (blood/plasma/antiserum) that contains antibodies.

Accepted Answer

The answer of An integral part of serological testing is...

Question 7

Three- dimensional images of specimens can be obtained using scanning electron microscopes.

Accepted Answer

A) True 
 B)False

Question 8

Compare and contrast the light microscope with the electron microscope.

Accepted Answer

Both the light microscope and the electr

Question 9

Viruses are not included in the taxonomic scheme proposed by Carl Woese because they lack

Accepted Answer

A)  cytoplasm. 
B)  genetic material. 
C)  ribosomal RNA. 
D)  lipid membranes. 
E)  proteins. 
A)  cytoplasm. 
B)  genetic material. 
C)  ribosomal RNA. 
D)  lipid membranes. 
E)  proteins.

Question 10

The stain makes use of malachite green.

Accepted Answer

A)  flagellar 
B)  acid- fast 
C)  electron microscopy 
D)  endospore 
E)  negative 
A)  flagellar 
B)  acid- fast 
C)  electron microscopy 
D)  endospore 
E)  negative

Question 11

You are shown a micrograph from a light microscope in which the specimens appear bright compared to the background. The micrograph is probably from a(n) microscope.

Accepted Answer

A)  phase- contrast 
B)  bright- field 
C)  Nomarski 
D)  atomic force 
E)  dark- field 
A)  phase- contrast 
B)  bright- field 
C)  Nomarski 
D)  atomic force 
E)  dark- field

Question 12

Light rays that are in phase reinforce each other and produce a brighter image.

Accepted Answer

A) True 
 B)False

Question 13

A (decolorizer/mordant/fixer) is a substance that binds to a dye and makes it less soluble.

Accepted Answer

The answer of A (decolorizer/mordant/fixer) is a substance that binds...

Question 14

Why does immersion oil improve resolution?

Accepted Answer

A)  It increases numerical aperture and maintains a uniform light speed. 
B)  It increases the angle of refraction of the light. 
C)  It increases the numerical aperture. 
D)  It decreases the working distance. 
E)  It allows light to travel at a uniform speed on its way to the lens. 
A)  It increases numerical aperture and maintains a uniform light speed. 
B)  It increases the angle of refraction of the light. 
C)  It increases the numerical aperture. 
D)  It decreases the working distance. 
E)  It allows light to travel at a uniform speed on its way to the lens.

Question 15

In Gram staining, ethanol- acetone is used as a

Accepted Answer

A)  counterstain. 
B)  drying agent. 
C)  mordant. 
D)  decolorizing agent. 
E)  primary stain. 
A)  counterstain. 
B)  drying agent. 
C)  mordant. 
D)  decolorizing agent. 
E)  primary stain.

Question 16

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

Accepted Answer

A)  proteins 
B)  DNA 
C)  transfer RNA 
D)  membrane lipids 
E)  ribosomal RNA 
A)  proteins 
B)  DNA 
C)  transfer RNA 
D)  membrane lipids 
E)  ribosomal RNA

Question 17

The limits of resolution of atomic force microscopes is in the range of

Accepted Answer

A)  micrometers (µm). 
B)  millimeters (mm). 
C)  nanometers (nm). 
D)  meters (m). 
E)  centimeters (cm). 
A)  micrometers (µm). 
B)  millimeters (mm). 
C)  nanometers (nm). 
D)  meters (m). 
E)  centimeters (cm).

Question 18

Carl Woese and his colleagues proposed the (phylum/domain ), a taxon that contains multiple kingdoms.

Accepted Answer

The answer of Carl Woese and his colleagues proposed the...

Question 19

The rules of naming organisms are called

Accepted Answer

A)  binomials. 
B)  identification. 
C)  nomenclature. 
D)  classification. 
E)  taxonomy. 
A)  binomials. 
B)  identification. 
C)  nomenclature. 
D)  classification. 
E)  taxonomy.

Question 20

You are a scientist studying the highly specific interactions of bacteriophages with their host cells when they first encounter the cell. Discuss what microscope(s) and preparation procedures you might use for this study.

Accepted Answer

A scanning electron microscope can produ

Exam 4: Microscopy, Staining, and Classification

A sample from a patient is prepared using the Gomori methenamine silver stain. What type of microbe is suspected of being present?

Which of the following is NOT a characteristic of a genus name?

Scanning tunneling microscopes map the topography of a specimen by detecting the deflection of the probe tip.

Species and strains of microbes can be distinguished from one another phenotypically using

In the Gram stain procedure, iodine serves as a

An integral part of serological testing is the use of a solution called (blood/plasma/antiserum) that contains antibodies.

Three- dimensional images of specimens can be obtained using scanning electron microscopes.

Compare and contrast the light microscope with the electron microscope.

Viruses are not included in the taxonomic scheme proposed by Carl Woese because they lack

The stain makes use of malachite green.

You are shown a micrograph from a light microscope in which the specimens appear bright compared to the background. The micrograph is probably from a(n) microscope.

Light rays that are in phase reinforce each other and produce a brighter image.

A (decolorizer/mordant/fixer) is a substance that binds to a dye and makes it less soluble.

Why does immersion oil improve resolution?

In Gram staining, ethanol- acetone is used as a

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

The limits of resolution of atomic force microscopes is in the range of

Carl Woese and his colleagues proposed the (phylum/domain ), a taxon that contains multiple kingdoms.

The rules of naming organisms are called

You are a scientist studying the highly specific interactions of bacteriophages with their host cells when they first encounter the cell. Discuss what microscope(s) and preparation procedures you might use for this study.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant Dna Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Disease, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram-Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic Dna Viruses

Pathogenic Rna Viruses

Applied and Environmental Microbiology

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