Exam 15: Histocompatibility Laboratory Techniques and Applications

PCR sequence specific oligonucleotide probe methods are usually preferred for large volumes of specimens over PCR sequence specific primer methods.

It is still normal a year after progenitor cell transplant to perform STR analysis and find both donor and recipient DNA in peripheral blood white cells.

When DNA has been extracted from a sample for HLA molecular typing

What is a short tandem repeat (STR), and how can it be used to determine progenitor cell engraftment?

What is an important advantage of HLA testing by molecular methods?

HLA typing is acceptable on lymphocyte preparations in which >95% of the cells can ingest the dye ________ ________.

How can the sensitivity of the cross- match between the recipient's serum and the donor's cells be enhanced?

Which process below includes all the controls utilized to make sure that a particular laboratory analysis is performing properly?

If a transplant is going to fail due to immune attack, it will be because of recipient production of antibodies or cytotoxic T cells.

The complement dependent cytotoxicity method of HLA testing can be used for

Gels for HLA typing by PCR should be interpreted by at least________ (how many?)people.

Traditional HLA typing always requires viable cells.

Personnel who work in HLA laboratories earn certification from the

For CLIA compliance, HLA testing labs can be inspected by

Which of the following is (are)TRUE regarding acceptable specimens for HLA typing by serological methods?

An advantage of flow cytometry cross- matching is that B cells and T cells do not have to be separated in lymphocyte preparations prior to analysis.

For HLA typing, the cell of choice is the ________ .

If a unrelated donor is being considered for progenitor cell transplant, which molecular HLA typing method below is required before final transplant can occur?

How can monocytes be removed from lymphocyte preparations?

After progenitor cell transplant, if STR DNA regions from both donor and recipient are detected, this is called a genetic ________.