Question 1

How does chemical degradation DNA sequencing (Maxam- Gilbert)differ from the dideoxy chain termination (Sanger)method?

Accepted Answer

A) Agarose gel is used for electrophoresis instead of high resolution polyacrylamide gel. 
B) Ethidium bromide is used to visualize the DNA fragments instead of radioactive sulfur or phosphorus. 
C) A lower ratio of dideoxy:deoxy nucleotides is used. 
D) "Rare cutter" restriction enzymes or nucleotide chemical modification are used to localize each base. 
A) Agarose gel is used for electrophoresis instead of high resolution polyacrylamide gel. 
B) Ethidium bromide is used to visualize the DNA fragments instead of radioactive sulfur or phosphorus. 
C) A lower ratio of dideoxy:deoxy nucleotides is used. 
D) "Rare cutter" restriction enzymes or nucleotide chemical modification are used to localize each base. D

Question 2

Which enzyme below links the discontinuous strands being synthesized in the 3' to 5' direction?

Accepted Answer

A) RNA polymerase 
B) DNA polymerase 
C) DNA gyrase 
D) DNA helicase 
E) DNA ligase 
A) RNA polymerase 
B) DNA polymerase 
C) DNA gyrase 
D) DNA helicase 
E) DNA ligase E

Question 3

What is the purpose of RNase in a DNA extraction procedure?

Accepted Answer

A) To digest the ribosomal RNA 
B) To stabilize DNA within the preparation 
C) To stabilize RNA within the preparation 
D) To remove DNA from the preparation 
E) To remove RNA from the preparation 
A) To digest the ribosomal RNA 
B) To stabilize DNA within the preparation 
C) To stabilize RNA within the preparation 
D) To remove DNA from the preparation 
E) To remove RNA from the preparation E

Question 4

A convenient method of creating DNA probes is called

Accepted Answer

A) nick translation. 
B) in situ hybridization. 
C) Western blotting. 
D) Southern blotting. 
E) Northern blotting. 
A) nick translation. 
B) in situ hybridization. 
C) Western blotting. 
D) Southern blotting. 
E) Northern blotting.

Question 5

When two pieces of complementary DNA from different sources anneal together, the resulting structure is called a primer.

Accepted Answer

A) True 
 B)False

Question 6

In a DNA extraction procedure, what will a mixture of phenol and chloroform extract?

Accepted Answer

A) Protein 
B) DNA 
C) RNA 
D) All of the above 
A) Protein 
B) DNA 
C) RNA 
D) All of the above

Question 7

What is the purpose of EDTA in a DNA extraction procedure?

Accepted Answer

A) To denature proteins 
B) To convert double- stranded DNA to single- stranded DNA 
C) To denature RNA 
D) To dissolve the nuclear membrane 
E) To inhibit DNase 
A) To denature proteins 
B) To convert double- stranded DNA to single- stranded DNA 
C) To denature RNA 
D) To dissolve the nuclear membrane 
E) To inhibit DNase

Question 8

Each of the following is TRUE regarding mRNA except

Accepted Answer

A) a single strand of mRNA may be translated by ribosomes only once. 
B) the 3' end of mRNA in eukaryotes consists of multiple adenines, the "poly- A tail." 
C) in eukaryotes, mRNA must undergo posttranscription processing to remove introns and join exons before it can be translated. 
D) translation of mRNA in prokaryotes can occur even before the mRNA strand is completely synthesized. 
A) a single strand of mRNA may be translated by ribosomes only once. 
B) the 3' end of mRNA in eukaryotes consists of multiple adenines, the "poly- A tail." 
C) in eukaryotes, mRNA must undergo posttranscription processing to remove introns and join exons before it can be translated. 
D) translation of mRNA in prokaryotes can occur even before the mRNA strand is completely synthesized.

Question 9

Which of the following is true regarding the structure of deoxyribonucleic acid (DNA)?

Accepted Answer

A) The "sides" of the helix are composed of deoxyribose and sulfate groups. 
B) The structure is a double helix. 
C) Regarding the nitrogeneous bases, adenine is always paired with guanine, and cytosine is always paired with thymine. 
D) All of the above. 
A) The "sides" of the helix are composed of deoxyribose and sulfate groups. 
B) The structure is a double helix. 
C) Regarding the nitrogeneous bases, adenine is always paired with guanine, and cytosine is always paired with thymine. 
D) All of the above.

Question 10

Restriction enzymes cut DNA at specific sites leaving only double- stranded fragments.

Accepted Answer

A) True 
 B)False

Question 11

The enzyme responsible for DNA transcription is DNA polymerase.

Accepted Answer

A) True 
 B)False

Question 12

When restriction enzymes cut a large strand of DNA, the resulting mixture is called a(n)

Accepted Answer

A) catenane. 
B) oligonucleotide. 
C) chimera. 
D) digest. 
A) catenane. 
B) oligonucleotide. 
C) chimera. 
D) digest.

Question 13

Each word below is paired correctly with its definition below except

Accepted Answer

A) intron/portion of mRNA that is translated into protein. 
B) transcription/conversion of DNA sequence to a mRNA sequence. 
C) codon/sequence of three nucleotides on mRNA that corresponds to a particular amino acid. 
D) translation/conversion of an mRNA sequence to a protein sequence. 
E) anticodon/sequence of three nucleotides on tRNA that corresponds to a particular codon sequence on mRNA. 
A) intron/portion of mRNA that is translated into protein. 
B) transcription/conversion of DNA sequence to a mRNA sequence. 
C) codon/sequence of three nucleotides on mRNA that corresponds to a particular amino acid. 
D) translation/conversion of an mRNA sequence to a protein sequence. 
E) anticodon/sequence of three nucleotides on tRNA that corresponds to a particular codon sequence on mRNA.

Question 14

A sequence specific endonuclease that cuts DNA at a particular nucleotide sequence is also called a________ .

Accepted Answer

The answer of A sequence specific endonuclease that cuts DNA...

Question 15

Separating the two strands of DNA in a double helix is relatively easy because________ bonds join the nitrogenous bases.

Accepted Answer

The answer of Separating the two strands of DNA in...

Question 16

All of the following can be used to lyse cells except

Accepted Answer

A) sarkosyl. 
B) diethylpyrocarbonate. 
C) lysozyme. 
D) Triton- X. 
E) guanidium isothiocyanate. 
A) sarkosyl. 
B) diethylpyrocarbonate. 
C) lysozyme. 
D) Triton- X. 
E) guanidium isothiocyanate.

Question 17

Which reagent below is necessary to construct chimeric DNA?

Accepted Answer

A) Triton- X 
B) Cesium chloride 
C) Restriction enzyme 
D) Ethanol 
E) DNA polymerase 
A) Triton- X 
B) Cesium chloride 
C) Restriction enzyme 
D) Ethanol 
E) DNA polymerase

Question 18

A common technique for detecting radioactivity in nucleic acid fragments is autoradiography.

Accepted Answer

A) True 
 B)False

Question 19

The unit of DNA comprised of a combination of a deoxyribose molecule, phosphate group, and nitrogeneous base is called a ________ .

Accepted Answer

The answer of The unit of DNA comprised of a...

Question 20

What is the first step in DNA purification from whole cells?

Accepted Answer

A) Phenol:chloroform extraction 
B) Cell lysis 
C) Addition of RNase 
D) Ethanol precipitation 
A) Phenol:chloroform extraction 
B) Cell lysis 
C) Addition of RNase 
D) Ethanol precipitation

How does chemical degradation DNA sequencing (Maxam- Gilbert)differ from the dideoxy chain termination (Sanger)method?

Which enzyme below links the discontinuous strands being synthesized in the 3' to 5' direction?

What is the purpose of RNase in a DNA extraction procedure?

A convenient method of creating DNA probes is called

When two pieces of complementary DNA from different sources anneal together, the resulting structure is called a primer.

In a DNA extraction procedure, what will a mixture of phenol and chloroform extract?

What is the purpose of EDTA in a DNA extraction procedure?

Each of the following is TRUE regarding mRNA except

Which of the following is true regarding the structure of deoxyribonucleic acid (DNA)?

Restriction enzymes cut DNA at specific sites leaving only double- stranded fragments.

The enzyme responsible for DNA transcription is DNA polymerase.

When restriction enzymes cut a large strand of DNA, the resulting mixture is called a(n)

Each word below is paired correctly with its definition below except

A sequence specific endonuclease that cuts DNA at a particular nucleotide sequence is also called a________ .

Separating the two strands of DNA in a double helix is relatively easy because________ bonds join the nitrogenous bases.

All of the following can be used to lyse cells except

Which reagent below is necessary to construct chimeric DNA?

A common technique for detecting radioactivity in nucleic acid fragments is autoradiography.

The unit of DNA comprised of a combination of a deoxyribose molecule, phosphate group, and nitrogeneous base is called a ________ .

What is the first step in DNA purification from whole cells?

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Amplification Techniques

Molecular Applications in Cytogenetics

Histocompatibility Laboratory Techniques and Applications

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Exam 12: Hybridization Techniques

How does chemical degradation DNA sequencing (Maxam- Gilbert)differ from the dideoxy chain termination (Sanger)method?

Which enzyme below links the discontinuous strands being synthesized in the 3' to 5' direction?

What is the purpose of RNase in a DNA extraction procedure?

A convenient method of creating DNA probes is called

When two pieces of complementary DNA from different sources anneal together, the resulting structure is called a primer.

In a DNA extraction procedure, what will a mixture of phenol and chloroform extract?

What is the purpose of EDTA in a DNA extraction procedure?

Each of the following is TRUE regarding mRNA except

Which of the following is true regarding the structure of deoxyribonucleic acid (DNA)?

Restriction enzymes cut DNA at specific sites leaving only double- stranded fragments.

The enzyme responsible for DNA transcription is DNA polymerase.

When restriction enzymes cut a large strand of DNA, the resulting mixture is called a(n)

Each word below is paired correctly with its definition below except

A sequence specific endonuclease that cuts DNA at a particular nucleotide sequence is also called a________ .

Separating the two strands of DNA in a double helix is relatively easy because________ bonds join the nitrogenous bases.

All of the following can be used to lyse cells except

Which reagent below is necessary to construct chimeric DNA?

A common technique for detecting radioactivity in nucleic acid fragments is autoradiography.

The unit of DNA comprised of a combination of a deoxyribose molecule, phosphate group, and nitrogeneous base is called a ________ .

What is the first step in DNA purification from whole cells?

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Amplification Techniques

Molecular Applications in Cytogenetics

Histocompatibility Laboratory Techniques and Applications

Filters