Question 1

FISH procedures are only accurate on metaphase cells.

Accepted Answer

A) True 
 B)False  False

Question 2

The acceptability for FISH analysis of cells on a prepared slide should be evaluated by

Accepted Answer

A) polarized light microscopy. 
B) electron microscopy. 
C) phase contrast microscopy. 
D) light microscopy. 
E) fluorescent microscopy. 
A) polarized light microscopy. 
B) electron microscopy. 
C) phase contrast microscopy. 
D) light microscopy. 
E) fluorescent microscopy. C

Question 3

Diseases caused by disruption of adjacent groups of genes are called ________ ________ syndromes.

Accepted Answer

contiguous gene

Question 4

Describe cell preparation for classic cytogenetic analysis.

Accepted Answer

1. Viable, nucleated cells are grown in

Question 5

For which of the testing situations listed is FISH (fluorescent in situ hybridization)most appropriate?

Accepted Answer

A) A complete chromosome count with examination of chromosomes for abnormalities must be done. 
B) A previously undescribed genetic defect is suspected. 
C) Karyotype appears normal by classic cytogenetics but phenotype suggests genetic abnormality. 
D) All of the above. 
A) A complete chromosome count with examination of chromosomes for abnormalities must be done. 
B) A previously undescribed genetic defect is suspected. 
C) Karyotype appears normal by classic cytogenetics but phenotype suggests genetic abnormality. 
D) All of the above.

Question 6

When indirectly labeled probes are used in FISH, what intermediary molecule(s)are usually used on the probes?

Accepted Answer

A) Digoxigenin 
B) Haptens 
C) Biotin 
D) All of the above 
A) Digoxigenin 
B) Haptens 
C) Biotin 
D) All of the above

Question 7

A clinical lab scientist was performing a cytogenetic analysis, and 10 of the chromosome spreads she analyzed had 46 normal chromosomes. In the 11th spread, she noticed 47 chromosomes. What is the first thing she should do?

Accepted Answer

A) Repeat the slide preparation to exclude errors that would cause chromosome artifacts. 
B) Repeat the slide preparation with a different stain. 
C) Examine more chromosomes spreads using to FISH try to confirm the presence of a genetic mosaic. 
D) Report a normal karyotype of 46 chromosomes. 
A) Repeat the slide preparation to exclude errors that would cause chromosome artifacts. 
B) Repeat the slide preparation with a different stain. 
C) Examine more chromosomes spreads using to FISH try to confirm the presence of a genetic mosaic. 
D) Report a normal karyotype of 46 chromosomes.

Question 8

Explain the difference between the two FISH Class I probes: alpha satellite and telomere probes.

Accepted Answer

Class I probes are generated against rep

Question 9

The best means of detecting contiguous gene syndromes is conventional cytogenetic analysis.

Accepted Answer

A) True 
 B)False

Question 10

Cytogenetic and FISH results should be reported using the International Classification of Disease (ICD)nomenclature.

Accepted Answer

A) True 
 B)False

Question 11

The composition of an individual's chromosomes is called the ________ .

Accepted Answer

The answer of The composition of an individual's chromosomes is...

Question 12

Define conditions of stringency as it relates to work with probes in FISH.

Accepted Answer

Hybridization of probe to target DNA is

Question 13

The class of FISH probe that consists of repetitive or satellite sequences is

Accepted Answer

A) Class I. 
B) Class II. 
C) Class III. 
D) Class IV. 
A) Class I. 
B) Class II. 
C) Class III. 
D) Class IV.

Question 14

When slides are prepared for FISH analysis,

Accepted Answer

A) they must be used immediately for optimal sensitivity. 
B) they can be stored at 37° C prior to analysis. 
C) they can be stored at - 4° C prior to analysis. 
D) they can be stored at 60° C prior to analysis. 
E) they can be stored at - 20° C prior to analysis. 
A) they must be used immediately for optimal sensitivity. 
B) they can be stored at 37° C prior to analysis. 
C) they can be stored at - 4° C prior to analysis. 
D) they can be stored at 60° C prior to analysis. 
E) they can be stored at - 20° C prior to analysis.

Question 15

The most useful probe to use for enumerating the number of chromosomes in a products of conception specimen is a telomere probe.

Accepted Answer

A) True 
 B)False

Question 16

Whole chromosome paints consist of

Accepted Answer

A) a mixture of DNA probes to various repeated internal sequences within the chromosome. 
B) a single DNA probe that spans the length of the entire intact chromosome. 
C) a mixture of probes to both repetitive and unique sequences within a particular chromosome. 
D) a mixture of alpha- satellite and telomere probes unique to that particular chromosome. 
A) a mixture of DNA probes to various repeated internal sequences within the chromosome. 
B) a single DNA probe that spans the length of the entire intact chromosome. 
C) a mixture of probes to both repetitive and unique sequences within a particular chromosome. 
D) a mixture of alpha- satellite and telomere probes unique to that particular chromosome.

Question 17

Explain the function of counterstain in a FISH procedure and how the most appropriate counterstain is chosen.

Accepted Answer

Although bound probes emit fluorescent s

Question 18

What is the major advantage of using uncultured cells for FISH analysis?

Accepted Answer

A) More metaphase cells for analysis 
B) Decreased turnaround time 
C) Decreased false positives 
D) More viable cells for analysis 
A) More metaphase cells for analysis 
B) Decreased turnaround time 
C) Decreased false positives 
D) More viable cells for analysis

Question 19

Which of the following is (are)disadvantage(s)of using directly labeled probes in FISH?

Accepted Answer

A) Less signal strength 
B) Less background staining 
C) Fewer steps in the procedure 
D) Less nonspecific binding 
E) All of the above 
A) Less signal strength 
B) Less background staining 
C) Fewer steps in the procedure 
D) Less nonspecific binding 
E) All of the above

Question 20

The acceptability for FISH analysis of cells on a prepared slide is most influenced by

Accepted Answer

A) temperature and humidity while fixed cells are drying on the slide. 
B) pH of the fixative solution. 
C) ionic strength of the fixative solution. 
D) detergent used to permeabilize the cell and nuclear membranes. 
E) detergent used to rupture the cell and nuclear membranes. 
A) temperature and humidity while fixed cells are drying on the slide. 
B) pH of the fixative solution. 
C) ionic strength of the fixative solution. 
D) detergent used to permeabilize the cell and nuclear membranes. 
E) detergent used to rupture the cell and nuclear membranes.

FISH procedures are only accurate on metaphase cells.

The acceptability for FISH analysis of cells on a prepared slide should be evaluated by

Diseases caused by disruption of adjacent groups of genes are called syndromes.

Describe cell preparation for classic cytogenetic analysis.

For which of the testing situations listed is FISH (fluorescent in situ hybridization)most appropriate?

When indirectly labeled probes are used in FISH, what intermediary molecule(s)are usually used on the probes?

A clinical lab scientist was performing a cytogenetic analysis, and 10 of the chromosome spreads she analyzed had 46 normal chromosomes. In the 11th spread, she noticed 47 chromosomes. What is the first thing she should do?

Explain the difference between the two FISH Class I probes: alpha satellite and telomere probes.

The best means of detecting contiguous gene syndromes is conventional cytogenetic analysis.

Cytogenetic and FISH results should be reported using the International Classification of Disease (ICD)nomenclature.

The composition of an individual's chromosomes is called the ________ .

Define conditions of stringency as it relates to work with probes in FISH.

The class of FISH probe that consists of repetitive or satellite sequences is

When slides are prepared for FISH analysis,

The most useful probe to use for enumerating the number of chromosomes in a products of conception specimen is a telomere probe.

Whole chromosome paints consist of

Explain the function of counterstain in a FISH procedure and how the most appropriate counterstain is chosen.

What is the major advantage of using uncultured cells for FISH analysis?

Which of the following is (are)disadvantage(s)of using directly labeled probes in FISH?

The acceptability for FISH analysis of cells on a prepared slide is most influenced by

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Hybridization Techniques

Amplification Techniques

Histocompatibility Laboratory Techniques and Applications

Filters

Exam 14: Molecular Applications in Cytogenetics

FISH procedures are only accurate on metaphase cells.

The acceptability for FISH analysis of cells on a prepared slide should be evaluated by

Diseases caused by disruption of adjacent groups of genes are called ________ ________ syndromes.

Describe cell preparation for classic cytogenetic analysis.

For which of the testing situations listed is FISH (fluorescent in situ hybridization)most appropriate?

When indirectly labeled probes are used in FISH, what intermediary molecule(s)are usually used on the probes?

A clinical lab scientist was performing a cytogenetic analysis, and 10 of the chromosome spreads she analyzed had 46 normal chromosomes. In the 11th spread, she noticed 47 chromosomes. What is the first thing she should do?

Explain the difference between the two FISH Class I probes: alpha satellite and telomere probes.

The best means of detecting contiguous gene syndromes is conventional cytogenetic analysis.

Cytogenetic and FISH results should be reported using the International Classification of Disease (ICD)nomenclature.

The composition of an individual's chromosomes is called the ________ .

Define conditions of stringency as it relates to work with probes in FISH.

The class of FISH probe that consists of repetitive or satellite sequences is

When slides are prepared for FISH analysis,

The most useful probe to use for enumerating the number of chromosomes in a products of conception specimen is a telomere probe.

Whole chromosome paints consist of

Explain the function of counterstain in a FISH procedure and how the most appropriate counterstain is chosen.

What is the major advantage of using uncultured cells for FISH analysis?

Which of the following is (are)disadvantage(s)of using directly labeled probes in FISH?

The acceptability for FISH analysis of cells on a prepared slide is most influenced by

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Clinical Applications of Flow Cytometry

Hybridization Techniques

Amplification Techniques

Histocompatibility Laboratory Techniques and Applications

Filters

Diseases caused by disruption of adjacent groups of genes are called syndromes.