Question 1

Using flow cytometry, cell markers can be detected that will determine a cell's

Accepted Answer

A) lineage. 
B) surface marker expression. 
C) maturational stage. 
D) All of the above. 
A) lineage. 
B) surface marker expression. 
C) maturational stage. 
D) All of the above. D

Question 2

Each of the following is a step in screening organ recipient serum for HLA antibodies except

Accepted Answer

A) Step 1: A panel of latex beads is coated with HLA antigens. 
B) Step 2: Recipient serum is mixed with each HLA antigen. 
C) Step 5: The latex beads are put in the flow cytometer and analyzed for agglutination based on increased forward scatter of agglutinated particles. 
D) Step 4: Fluorochrome- labeled antihuman globulin is mixed with the latex beads. 
E) Step 3: After serum has incubated with the beads, they are washed to remove all excess serum. 
A) Step 1: A panel of latex beads is coated with HLA antigens. 
B) Step 2: Recipient serum is mixed with each HLA antigen. 
C) Step 5: The latex beads are put in the flow cytometer and analyzed for agglutination based on increased forward scatter of agglutinated particles. 
D) Step 4: Fluorochrome- labeled antihuman globulin is mixed with the latex beads. 
E) Step 3: After serum has incubated with the beads, they are washed to remove all excess serum. C

Question 3

In a flow cytometer, the amount of light scattered by a cell at a 90° angle (side scatter)is detected by a ________ .

Accepted Answer

A) photomultiplier tube. 
B) tungsten lamp. 
C) phototube. 
D) photodiode. 
E) laser. 
A) photomultiplier tube. 
B) tungsten lamp. 
C) phototube. 
D) photodiode. 
E) laser. A

Question 4

Explain how flow cytometry and hematology cell counting are both used to obtain absolute CD4+ lymphocyte counts.

Accepted Answer

Flow cytometry results are typically rep

Question 5

Which of the cells below should scatter the most light in the forward angle?

Accepted Answer

A) Monocyte 
B) Basophil 
C) Neutrophil 
D) Lymphocyte 
E) Eosinophil 
A) Monocyte 
B) Basophil 
C) Neutrophil 
D) Lymphocyte 
E) Eosinophil

Question 6

In the scatterplot shown, in which population would eosinophils be found?

Accepted Answer

A) A 
B) B 
C) C 
D) None of the above 
A) A 
B) B 
C) C 
D) None of the above

Question 7

Effective antibody reagents in flow cytometry are produced by hybridoma cells and are________
.

Accepted Answer

The answer of Effective antibody reagents in flow cytometry are...

Question 8

When different flourochromes are used in flow cytometry that have overlapping emission spectra, the interference can be electronically subtracted using a process called fluorescence ________
.

Accepted Answer

The answer of When different flourochromes are used in flow...

Question 9

Which of the following is(are)essential characteristics of the fluidic system of a flow cytometer?

Accepted Answer

A) Adjust the rate of diluent flow depending on cell count (i.e., slower flow for specimens with high counts). 
B) Create a hydrodynamically focused sheath fluid that will cause the cells to flow past the laser in single file. 
C) Maintain a slow moving, low pressure solution flow such that there is sufficient time to analyze each cell. 
D) All of the above. 
A) Adjust the rate of diluent flow depending on cell count (i.e., slower flow for specimens with high counts). 
B) Create a hydrodynamically focused sheath fluid that will cause the cells to flow past the laser in single file. 
C) Maintain a slow moving, low pressure solution flow such that there is sufficient time to analyze each cell. 
D) All of the above.

Question 10

Labeled monoclonal antifetal hemoglobin can be mixed with fixed red blood cells to detect fetal red cells.

Accepted Answer

A) True 
 B)False

Question 11

Flow cytometry can be used to determine the presence of fetal cells in a blood specimen by using

Accepted Answer

A) monoclonal antibody to hemoglobin A1. 
B) alkaline elution. 
C) monoclonal antibody to hemoglobin F. 
D) acid elution. 
A) monoclonal antibody to hemoglobin A1. 
B) alkaline elution. 
C) monoclonal antibody to hemoglobin F. 
D) acid elution.

Question 12

When stem cell harvests are done for hematopoietic progenitors, flow cytometry is done for the ________ marker as it is highly associated with elevated levels of progenitor cells.

Accepted Answer

The answer of When stem cell harvests are done for...

Question 13

Reticulocytes can be analyzed using flow cytometry when they are stained with ethidium bromide.

Accepted Answer

A) True 
 B)False

Question 14

When antiretroviral therapy is successful in AIDS patients, the count of CD4+ lymphocytes will increase.

Accepted Answer

A) True 
 B)False

Question 15

All of the following are TRUE regarding specimen handling for flow cytometry except

Accepted Answer

A) ammonium chloride is used to lyse granulocytes when mononuclear cells need to be analyzed. 
B) tissues must be homogenized into single cell suspensions. 
C) a minimum number of cells must be present for analysis to be accurate. 
D) specimens must be processed promptly to insure cell viability. 
E) cell surface antigens must be stabilized with paraformaldehyde. 
A) ammonium chloride is used to lyse granulocytes when mononuclear cells need to be analyzed. 
B) tissues must be homogenized into single cell suspensions. 
C) a minimum number of cells must be present for analysis to be accurate. 
D) specimens must be processed promptly to insure cell viability. 
E) cell surface antigens must be stabilized with paraformaldehyde.

Question 16

It is desirable before a transplant that the donor cells be cross- matched with the recipient's serum to rule out preexisting antibodies in the recipient. What advantage is there in doing the cross- match by flow cytometry?

Accepted Answer

A) The equipment is less expensive. 
B) Flow cytometry is more sensitive that other techniques. 
C) Donor cells do not have to be viable. 
D) Flow cytometry is more specific that other techniques. 
A) The equipment is less expensive. 
B) Flow cytometry is more sensitive that other techniques. 
C) Donor cells do not have to be viable. 
D) Flow cytometry is more specific that other techniques.

Question 17

How is flow cytometry used to determine if a donor bone marrow aspirate or stem cell harvest is adequate to repopulate a bone marrow?

Accepted Answer

An adequate number of pluripotent stem c

Question 18

What is the usual light source for a flow cytometer?

Accepted Answer

A) Ultraviolet lamp 
B) Deuterium lamp 
C) Tungsten lamp 
D) Laser 
A) Ultraviolet lamp 
B) Deuterium lamp 
C) Tungsten lamp 
D) Laser

Question 19

A flow cytometry analysis was done and the gate was set on the lymphocytes. Observe the analysis below of the cells in the gate possessing the CD4 and CD3 markers. In which quadrant are T helper cells found?

Accepted Answer

A) Quadrant A 
B) Quadrant B 
C) Quadrant C 
D) Quadrant D 
E) Quadrants B and D 
A) Quadrant A 
B) Quadrant B 
C) Quadrant C 
D) Quadrant D 
E) Quadrants B and D

Question 20

A flow cytometry analysis was done as shown in the figure with the gate set on red blood cells. What is the most likely interpretation of the analysis?

Accepted Answer

A) The red cells were all lysed in the preparation. 
B) The gate is contaminated with white blood cells. 
C) The patient may have a glucose- 6- phosphate dehydrogenase deficiency. 
D) The patient may have paroxysmal nocturnal hemoglobinuria. 
E) The reticulocyte population is elevated. 
A) The red cells were all lysed in the preparation. 
B) The gate is contaminated with white blood cells. 
C) The patient may have a glucose- 6- phosphate dehydrogenase deficiency. 
D) The patient may have paroxysmal nocturnal hemoglobinuria. 
E) The reticulocyte population is elevated.

Using flow cytometry, cell markers can be detected that will determine a cell's

Each of the following is a step in screening organ recipient serum for HLA antibodies except

In a flow cytometer, the amount of light scattered by a cell at a 90° angle (side scatter)is detected by a ________ .

Explain how flow cytometry and hematology cell counting are both used to obtain absolute CD4+ lymphocyte counts.

Which of the cells below should scatter the most light in the forward angle?

In the scatterplot shown, in which population would eosinophils be found?

Effective antibody reagents in flow cytometry are produced by hybridoma cells and are________ .

When different flourochromes are used in flow cytometry that have overlapping emission spectra, the interference can be electronically subtracted using a process called fluorescence ________ .

Which of the following is(are)essential characteristics of the fluidic system of a flow cytometer?

Labeled monoclonal antifetal hemoglobin can be mixed with fixed red blood cells to detect fetal red cells.

Flow cytometry can be used to determine the presence of fetal cells in a blood specimen by using

When stem cell harvests are done for hematopoietic progenitors, flow cytometry is done for the ________ marker as it is highly associated with elevated levels of progenitor cells.

Reticulocytes can be analyzed using flow cytometry when they are stained with ethidium bromide.

When antiretroviral therapy is successful in AIDS patients, the count of CD4+ lymphocytes will increase.

All of the following are TRUE regarding specimen handling for flow cytometry except

It is desirable before a transplant that the donor cells be cross- matched with the recipient's serum to rule out preexisting antibodies in the recipient. What advantage is there in doing the cross- match by flow cytometry?

How is flow cytometry used to determine if a donor bone marrow aspirate or stem cell harvest is adequate to repopulate a bone marrow?

What is the usual light source for a flow cytometer?

A flow cytometry analysis was done and the gate was set on the lymphocytes. Observe the analysis below of the cells in the gate possessing the CD4 and CD3 markers. In which quadrant are T helper cells found?

A flow cytometry analysis was done as shown in the figure with the gate set on red blood cells. What is the most likely interpretation of the analysis?

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Hybridization Techniques

Amplification Techniques

Molecular Applications in Cytogenetics

Histocompatibility Laboratory Techniques and Applications

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Exam 11: Clinical Applications of Flow Cytometry

Using flow cytometry, cell markers can be detected that will determine a cell's

Each of the following is a step in screening organ recipient serum for HLA antibodies except

In a flow cytometer, the amount of light scattered by a cell at a 90° angle (side scatter)is detected by a ________ .

Explain how flow cytometry and hematology cell counting are both used to obtain absolute CD4+ lymphocyte counts.

Which of the cells below should scatter the most light in the forward angle?

In the scatterplot shown, in which population would eosinophils be found?

Effective antibody reagents in flow cytometry are produced by hybridoma cells and are________ .

When different flourochromes are used in flow cytometry that have overlapping emission spectra, the interference can be electronically subtracted using a process called fluorescence ________ .

Which of the following is(are)essential characteristics of the fluidic system of a flow cytometer?

Labeled monoclonal antifetal hemoglobin can be mixed with fixed red blood cells to detect fetal red cells.

Flow cytometry can be used to determine the presence of fetal cells in a blood specimen by using

When stem cell harvests are done for hematopoietic progenitors, flow cytometry is done for the ________ marker as it is highly associated with elevated levels of progenitor cells.

Reticulocytes can be analyzed using flow cytometry when they are stained with ethidium bromide.

When antiretroviral therapy is successful in AIDS patients, the count of CD4+ lymphocytes will increase.

All of the following are TRUE regarding specimen handling for flow cytometry except

It is desirable before a transplant that the donor cells be cross- matched with the recipient's serum to rule out preexisting antibodies in the recipient. What advantage is there in doing the cross- match by flow cytometry?

How is flow cytometry used to determine if a donor bone marrow aspirate or stem cell harvest is adequate to repopulate a bone marrow?

What is the usual light source for a flow cytometer?

A flow cytometry analysis was done and the gate was set on the lymphocytes. Observe the analysis below of the cells in the gate possessing the CD4 and CD3 markers. In which quadrant are T helper cells found?

A flow cytometry analysis was done as shown in the figure with the gate set on red blood cells. What is the most likely interpretation of the analysis?

What Is Immunology

Overview of the Immune System

Immunoglobulins

Infection and Immunity: Hostparasite Relationships

The Complement System

Hypersensitivity

Immunologic Detection of Infectious Diseases

Autoimmune Diseases and Immunodeficiency Disorders

Transplant Immunology

Tumor Immunology

Hybridization Techniques

Amplification Techniques

Molecular Applications in Cytogenetics

Histocompatibility Laboratory Techniques and Applications

Filters