Question 1

In the context of molecular genetics, reverse transcription PCR (RT -PCR) refers to _.

Accepted Answer

A)  translating in the 3' to 5' direction 
B)  assembling a DNA sequence from an mRNA 
C)  making an amino acid sequence from a DNA sequence 
D)  assembling an RNA sequence from a DNA sequence 
E)  transcribing first, then translating 
A)  translating in the 3' to 5' direction 
B)  assembling a DNA sequence from an mRNA 
C)  making an amino acid sequence from a DNA sequence 
D)  assembling an RNA sequence from a DNA sequence 
E)  transcribing first, then translating B

Question 2

What is the specific application of reverse transcriptase in the preparation of cDNA?

Accepted Answer

synthesis of DNA to form an RNA -DNA duplex

Question 3

In a typical PCR, primers are used to cleave specific regions of the DNA template.

Accepted Answer

A) True 
 B)False  False

Question 4

Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot, one generally _.

Accepted Answer

A)  examines amino acid substitutions with radioactive probes 
B)  ligates DNA with DNA ligase 
C)  hybridizes filter -bound RNA with a DNA probe 
D)  hybridizes filter -bound DNA with a DNA probe 
E)  cleaves RNA with restriction endonucleases 
A)  examines amino acid substitutions with radioactive probes 
B)  ligates DNA with DNA ligase 
C)  hybridizes filter -bound RNA with a DNA probe 
D)  hybridizes filter -bound DNA with a DNA probe 
E)  cleaves RNA with restriction endonucleases

Question 5

A ddNTP, used often in DNA sequencing, lacks a(n) carbons.

Accepted Answer

A)  OH; 2'; 3' 
B)  carboxyl; 5'; 3' 
C)  methyl; 2'; 3' 
D)  OH; 2'; 5' 
E)  None of the listed answers is correct. 
A)  OH; 2'; 3' 
B)  carboxyl; 5'; 3' 
C)  methyl; 2'; 3' 
D)  OH; 2'; 5' 
E)  None of the listed answers is correct.

Question 6

Assume that you have cut h DNA with the restriction enzyme HindIII. You separate
the fragments on an agarose gel and stain the DNA with ethidium bromide. You notice that the intensity of the stain is less in the bands that have migrated closer to the "+"
pole. Give an explanation for this finding.

Accepted Answer

Because the smaller fragments

Question 7

Under ideal conditions, how many copies of all the sequences of the host genome should be represented in a genomic library?

Accepted Answer

At least one should be represented. Typi

Question 8

What is recombinant DNA technology? What are the safety issues related to recombinant DNA technology?

Accepted Answer

Recombinant DNA technology refers to the

Question 9

The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat -stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been
Heated to 95°C. Why would such a heat -stable polymerase be beneficial in PCR?

Accepted Answer

A)  Each cycle includes a "hot" denaturation phase (95°
C) , which separates the hydrogen bonds that hold the strands of the template DNA together. 
B)  Each cycle includes a "hot" denaturation phase (95°
C) , which serves to sterilize the culture. 
C)  Each cycle includes a "hot" saturation phase (95°
C) , which allows the primers to anneal to the target DNA. 
D)  Each cycle includes a "hot" denaturation phase (95°
C) , which activates the Taq polymerase. 
E)  More than one of the above are correct. 
A)  Each cycle includes a "hot" denaturation phase (95°
C) , which separates the hydrogen bonds that hold the strands of the template DNA together. 
B)  Each cycle includes a "hot" denaturation phase (95°
C) , which serves to sterilize the culture. 
C)  Each cycle includes a "hot" saturation phase (95°
C) , which allows the primers to anneal to the target DNA. 
D)  Each cycle includes a "hot" denaturation phase (95°
C) , which activates the Taq polymerase. 
E)  More than one of the above are correct.

Question 10

In which of the following biochemical reactions is it common to use ddNTPs (dideoxyribonucleoside triphosphates)?

Accepted Answer

A)  transfection 
B)  immunoprecipitation 
C)  DNA sequencing 
D)  plasmolysis 
E)  restriction digestion 
A)  transfection 
B)  immunoprecipitation 
C)  DNA sequencing 
D)  plasmolysis 
E)  restriction digestion

Question 11

Restriction endonucleases typically recognize palindromic DNA sequences and often generate "sticky ends" or single -stranded DNA overhangs at cut sites.

Accepted Answer

A) True 
 B)False

Question 12

The function of a ddNTP in DNA sequencing is to methylate guanine.

Accepted Answer

A) True 
 B)False

Question 13

The main purpose of a probe is its insertion in plasmid DNA.

Accepted Answer

A) True 
 B)False

Question 14

Assume that a plasmid (circular) is 3200 base pairs in length and has restriction sites at the following locations: 400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion.

Accepted Answer

A)  300, 700, 2200 
B)  400, 800, 1000 (2 of these) 
C)  400, 1200, 1600 
D)  300, 700, 1000, 1200 
E)  700, 400, 1400, 2600 
A)  300, 700, 2200 
B)  400, 800, 1000 (2 of these) 
C)  400, 1200, 1600 
D)  300, 700, 1000, 1200 
E)  700, 400, 1400, 2600

Question 15

In recombinant DNA technology, YAC, RFLP, and h have identical uses.

Accepted Answer

A) True 
 B)False

Question 16

How might cDNA libraries be used to assign particular genes to the diseased state of a cell?

Accepted Answer

One can use a comparison of cD

Question 17

What might be a reasonable function of restriction endonucleases in a bacterium, distinct from their use by molecular biologists?

Accepted Answer

Isolated from bacteria, restri

Question 18

List, in order, the steps usually followed in producing recombinant DNA molecules in a plasmid vector.

Accepted Answer

isolation of DNA (foreign and

Question 19

One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that _.

Accepted Answer

A)  each vector can take up only a relatively small fraction of the eukaryotic DNA 
B)  the host range of the vector is limited 
C)  each ligation product is sequence specific 
D)  lysogenic phages continue to integrate their DNA into the host chromosome, thus reducing the number of desired recombinant clones 
E)  each cosmid replicates in coordination with the host chromosome 
A)  each vector can take up only a relatively small fraction of the eukaryotic DNA 
B)  the host range of the vector is limited 
C)  each ligation product is sequence specific 
D)  lysogenic phages continue to integrate their DNA into the host chromosome, thus reducing the number of desired recombinant clones 
E)  each cosmid replicates in coordination with the host chromosome

Question 20

In the polymerase chain reaction, what is the purpose of the initial high temperature? What is the purpose of cooling in the second step?

Accepted Answer

to denature the targ

Exam 17: Recombinant Dna Technology

In the context of molecular genetics, reverse transcription PCR (RT -PCR) refers to _.

What is the specific application of reverse transcriptase in the preparation of cDNA?

In a typical PCR, primers are used to cleave specific regions of the DNA template.

Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot, one generally _.

A ddNTP, used often in DNA sequencing, lacks a(n) carbons.

Assume that you have cut h DNA with the restriction enzyme HindIII. You separate the fragments on an agarose gel and stain the DNA with ethidium bromide. You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole. Give an explanation for this finding.

Under ideal conditions, how many copies of all the sequences of the host genome should be represented in a genomic library?

What is recombinant DNA technology? What are the safety issues related to recombinant DNA technology?

In which of the following biochemical reactions is it common to use ddNTPs (dideoxyribonucleoside triphosphates)?

Restriction endonucleases typically recognize palindromic DNA sequences and often generate "sticky ends" or single -stranded DNA overhangs at cut sites.

The function of a ddNTP in DNA sequencing is to methylate guanine.

The main purpose of a probe is its insertion in plasmid DNA.

Assume that a plasmid (circular) is 3200 base pairs in length and has restriction sites at the following locations: 400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion.

In recombinant DNA technology, YAC, RFLP, and h have identical uses.

How might cDNA libraries be used to assign particular genes to the diseased state of a cell?

What might be a reasonable function of restriction endonucleases in a bacterium, distinct from their use by molecular biologists?

List, in order, the steps usually followed in producing recombinant DNA molecules in a plasmid vector.

One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that _.

In the polymerase chain reaction, what is the purpose of the initial high temperature? What is the purpose of cooling in the second step?

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Modifications of Mendelian Ratios

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Linkage and Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bactierophages

DNA Structure and Analysis

DNA Replication and Recombination

Chromosome Structure and Dna Sequence Organization

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation, Dna Repair, and Transposition

Regulation of Gene Expression

The Genetics of Cancer

Genomics and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Developmental Genetics

Quantitative Genetics and Multifactorial Traits

Population and Evolutionary Genetics

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