Exam 8: Genetic Analysis and Mapping in Bacteria and Bactierophages

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Assume that one counted 67 plaques on a bacterial plate where 0.1 ml of a 10 -5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage?

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Question 1

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67 × 105 × 10 = 6.7 × 107 pfu/ml (pfu = plaque -forming units)

Lysogeny is an important phenomenon in bacteria and phages. Briefly describe lysogeny.

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Question 2

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Lysogeny is the process in which a temperate bacteriophage infects a bacterial
cell and subsequently integrates its chromosome into the bacterial chromosome.

Lysogeny is most likely associated with transduction.

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(True/False)

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Question 3

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True

What is meant by the term cotransformation?

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Question 4

The "interrupted mating technique" provides a genetic map in Drosophila.

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Question 5

What term is used to describe the phenomenon in which a bacteriophage genome incorporates its genome into the chromosome of the host?

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Question 6

When a bacteriophage genome incorporates itself into the chromosome of the host, that phage genome is referred to as a(n)

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Question 7

A plaque is a substance that causes mutation in bacteria.

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Question 8

A form of bacterial recombination that involves a viral intermediate is called _.

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Question 9

Temperate phages are those that can enter either the or

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Question 10

Bacteriophages that cannot undergo lysogeny but can infect bacteria are called ________.

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Question 11

Name the typical phases of the bacterial growth cycle in liquid culture medium.

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Question 12

What are prototrophs?

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Question 13

Jacob, Wollman, and others developed a linkage map of E. coli that is based on time. What form of recombination is involved in generating a linkage map based on time?

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Question 14

Bacteriophages engage in two interactive cycles with bacteria. What are these cycles?

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Question 15

In what way was the interrupted mating technique used to generate a genetic map in E. coli based on time?

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Question 16

An Hfr cell can initiate chromosome transfer from one E. coli to another.

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Question 17

A symbiotic relationship between a phage and a bacterium apparently occurs in the process of lysogeny.

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Question 18

Cotransduction of genes is an indication that the genes are linked.

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Question 19

A bacteriophage that is capable of entering either a lytic or lysogenic cycle is called a(n) ________.

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Question 20

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Exam 8: Genetic Analysis and Mapping in Bacteria and Bactierophages

Assume that one counted 67 plaques on a bacterial plate where 0.1 ml of a 10 -5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage?

Lysogeny is an important phenomenon in bacteria and phages. Briefly describe lysogeny.

Lysogeny is most likely associated with transduction.

What is meant by the term cotransformation?

The "interrupted mating technique" provides a genetic map in Drosophila.

What term is used to describe the phenomenon in which a bacteriophage genome incorporates its genome into the chromosome of the host?

When a bacteriophage genome incorporates itself into the chromosome of the host, that phage genome is referred to as a(n)

A plaque is a substance that causes mutation in bacteria.

A form of bacterial recombination that involves a viral intermediate is called _.

Temperate phages are those that can enter either the or

Bacteriophages that cannot undergo lysogeny but can infect bacteria are called ________.

Name the typical phases of the bacterial growth cycle in liquid culture medium.

What are prototrophs?

Jacob, Wollman, and others developed a linkage map of E. coli that is based on time. What form of recombination is involved in generating a linkage map based on time?

Bacteriophages engage in two interactive cycles with bacteria. What are these cycles?

In what way was the interrupted mating technique used to generate a genetic map in E. coli based on time?

An Hfr cell can initiate chromosome transfer from one E. coli to another.

A symbiotic relationship between a phage and a bacterium apparently occurs in the process of lysogeny.

Cotransduction of genes is an indication that the genes are linked.

A bacteriophage that is capable of entering either a lytic or lysogenic cycle is called a(n) ________.

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Modifications of Mendelian Ratios

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Linkage and Chromosome Mapping in Eukaryotes

DNA Structure and Analysis

DNA Replication and Recombination

Chromosome Structure and Dna Sequence Organization

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation, Dna Repair, and Transposition

Regulation of Gene Expression

The Genetics of Cancer

Recombinant Dna Technology

Genomics and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Developmental Genetics

Quantitative Genetics and Multifactorial Traits

Population and Evolutionary Genetics

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