Exam 16: Cell Walls, the Extracellular Matrix, and Cell Interactions

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The X-linked variant of Charcot-Marie-Tooth disease is likely caused by mutations in a gene encoding a probable connexin 32. Suppose your laboratory is working with a human cell line called HeLa cells, which do not express connexins. Propose an experiment to demonstrate that the probable wild-type connexin 32 is actually a gap junction protein.

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How is proline hydroxylation in collagen encoded in the genome? What is the relationship between this encoding mechanism and scurvy?

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How many molecules of the gap junction protein form one open channel between two cells?

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What prevents collagen from assembling into fibrils inside the cell?

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The major cell surface receptor(s) responsible for the attachment of cells to the extracellular matrix is(are)

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The structural polysaccharide of fungal cell walls is the same as the one found in insect

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In plant cell walls, _______ are highly branched polysaccharides that are hydrogen-bonded to cellulose microfibrils.

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What are the four classes of cell adhesion molecules?

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The individual units of gap junctions are composed of _______ molecules.

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Vitamin C deficiency may lead to scurvy, a disease characterized by skin lesions and blood vessel hemorrhages due to weakened connective tissue. Vitamin C deficiency has this effect because the vitamin is

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The amino acid sequence of a collagen triple helix domain consists of Gly-X-Y repeats, in which X is frequently _______ and Y is frequently _______.

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Within the plant cell wall, cellulose microfibrils are embedded in a matrix consisting of proteins and two other types of polysaccharides: _______ and _______.

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Describe the structure of laminins.

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Pectins form a _______ network in plant cell walls.

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Basal laminae are formed primarily from type IV collagen and other proteins.

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The thin, sheetlike basal laminae are found

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Describe the structure of an integrin, as determined from the cDNA sequence isolated from fibroblasts by Hynes et al.

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If developing pre-nerve cells expressing only N-cadherin on their surfaces were mixed with epithelial cells expressing only E-cadherin, what would be the most likely result?

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Why is the term "basal lamina" preferred over the term "basement membrane"?

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Gap junctions allow passage of molecules that have a molecular weight of less than _______ daltons.

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