Exam 8: RNA Synthesis and Processing

Explain what TFIIH is, including its function in initiating transcription.

What are the 5 subunits that make up bacterial RNA polymerase, and which one(s), if any, are weakly bound to the complex?

Explain how alternative splicing of transformer (tra)in Drosophila leads to sex determination.

Which of the following is not part of the transcription complex?

All of the following are required for eukaryotic transcription in vitro except

RNA processing events (other than splicing) that alter the protein-coding sequences of some RNAs are called _______.

Spliced mRNAs have protective mechanisms, but introns are rapidly degraded in the

What is the general mechanism for degrading mature mRNAs in the cytoplasm?

Mitochondrial genes are transcribed by

The structure that is formed during termination of transcription due to an inverted GC-rich inverted repeat is known as a _______.

A major difference between eukaryotic and prokaryotic RNA polymerases is that eukaryotic polymerases

In vitro experiments have shown that formation of the preinitiation transcription complex on RNA polymerase II promoters requires the dephosphorylated form of the RNA polymerase II large subunit, whereas the phosphorylated form is associated with elongating complexes. Describe how the timing of phosphorylation might influence gene transcription.

What happens to the lariat-like structure that is excised during mRNA splicing?

RNA editing refers to RNA processing events (other than splicing) that alter the protein-coding sequences of some mRNAs.

Which of the following RNAs is least stable and has the shortest half-life?

Explain the two steps involved in pre-mRNA splicing.

In eukaryotes, transcription of mitochondrial genes uses typical nuclear RNA polymerases.

In prokaryotes, $\sigma$ factors are necessary for

Nucleosomes are an impediment to transcription in eukaryotic cells.

Through the process of _______, cells can produce different proteins from the same gene.