Exam 3: Bioenergetics and Metabolism

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Gluconeogenesis involves the conversion of pyruvate to glucose. In a formal sense, this is essentially the reversal of glycolysis. However, the actual reactions are different. Explain this.

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What is the definition of free-energy change?

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The _______ produced by the electron transport chain drives a flow of these ions through a complex of proteins called _______ to produce ATP from ADP and Pi.

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Traditionally, it was assumed that ATP synthesis in mitochondria would be via high-energy phosphate group transfer intermediates, but none have ever been found. How does the chemiosmotic theory explain their absence?

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Fatty acids are broken down in a stepwise process, _______ carbon(s) at a time.

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The potential energy stored in the proton gradient that drives oxidative phosphorylation is totally chemical in nature.

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What is the change in free energy ( Δ\Delta G) for the hydrolysis of ATP to ADP and Pi in the cell?

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Where in "typical" eukaryotic cells does the citric acid cycle occur? Be specific if the process or event occurs in only one part of an organelle. If you are not sure, give the location of the enzymes that catalyze the process.

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What is the definition of Gibbs free energy (G)?

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In humans, how many amino acids must be taken up in the diet?

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Suppose that in a cell culture experiment you add glucose uniformly labeled with carbon-14 to the culture medium. You then assay for the kinetics of carbon-14 incorporation into various amino acids. You find that carbon-14 is incorporated into histidine before it is incorporated into aspartate. Why?

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Nucleic acid synthesis occurs by polymerization of nucleotide monophosphates into DNA or RNA.

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In photosynthesis, energy from sunlight is used to drive the synthesis of ATP and NADPH.

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In plants, the molecule that captures energy from sunlight is

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The conversion of A into B has a Δ\Delta G of +13 kcal/mol, and the conversion of C into D has a Δ\Delta G of -25 kcal/mol. If the two reactions are coupled through an enzyme, such that A + C goes to B + D, what is the free energy change, and in which direction will the reaction proceed?

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Because fatty acids are synthesized by the stepwise addition of acetyl CoA derivatives, they differ in length by units of _______ carbon(s).

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Antimetabolite chemotherapy succeeds as a treatment for childhood leukemia because

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The production of one glucose from two pyruvates requires _______ more than is obtained through glycolysis.

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The reduction of atmospheric N2 to NH3 by certain species of bacteria is referred to as _______.

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A nucleotide is a

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