Exam 14: Networks of Gene Regulation

Match the missing words in the following phrase. Different biological processes are governed by different types of regulatory networks that often reflect the type of regulation needed. For example, the stress response in yeast is very [A] and involves a small number of transcription factors activating [B] genes. For very [C] processes such as the cell cycle, many transcription factors are involved in regulating [D] genes. Transcription networks are hierarchical, with [E] controlling large networks; these are in turn coordinated by [F].

Which of the following statements about polar mutations are true? Please select all that apply.

Fill in the blank. The biochemical process of breaking down a complex molecule into its simpler components is termed _____

Match the points on the bacterial growth curve with their descriptions. -Cell death exceeds cell division

Which of the following is the critical regulatory stage for the majority of genes?

Put the following steps in a ChIP experiment in order, beginning with the first -Cross-link DNA and proteins

Match the points on the bacterial growth curve with their descriptions. -Cells doubling at steady rate

Which of the statements about the transcription factors illustrated in the network diagram are true? Please select all that apply.

Attenuation is involved in the regulation of tryptophan production in E. coli. Which factors are involved in the tryptophan attenuation mechanism?

Match the protein binding patterns at the lac operon with the appropriate transcriptional outcomes. -CAP bound to DNA, lac repressor not bound to DNA

Tryptophan is involved in transcriptional regulation of the tryptophan-encoding genes. It does this by binding to the tryptophan repressor. Tryptophan is therefore a ________

Match the points on the bacterial growth curve with their descriptions. -Nutrients exhausted

Which of the following are limitations of ChIP experiments? Please select all that apply.

In ChIP, what sort of molecule is used to isolate chromatin attached to the transcription factor of interest

Operons are a key mechanism for co-regulation of genes in bacteria and eukaryotes.

Eukaryotic genes are controlled by more than one transcription factor, but each transcription factor is only involved in the transcription of one gene.

Fill in the blank. Many transcription factors target their own gene for regulation. This is termed ________________

Match the missing words in the following phrase. Tryptophan is an essential amino acid. Wild-type E. coli can synthesize their own tryptophan and are called [A]. Mutants that cannot synthesize an essential nutrient like tryptophan are called [B]. Transcription of the genes for tryptophan synthesis is not [C]: i.e., the genes are transcribed only when needed. Transcription is therefore [D]. When tryptophan is [E], the repressor [F] and transcription of the tryptophan mRNA proceeds. When tryptophan is [G], the repressor [H] and transcription is prevented.

What is a regulon?

Why is it difficult to accurately identify transcription factor binding sites by scanning the genome sequence? Please select all that apply.