Question 1

When E. coli host strain K-12 is infected by phage lambda K, the phage DNA is

Accepted Answer

A)  recognized as foreign and cleaved by a specific restriction endonuclease. 
B)  recognized as foreign and marked with a methylation pattern. 
C)  not recognized as foreign because it has the same methylation pattern as the E. coli genome 
D)  not recognized as foreign because of the phage restriction and modification system. 
A)  recognized as foreign and cleaved by a specific restriction endonuclease. 
B)  recognized as foreign and marked with a methylation pattern. 
C)  not recognized as foreign because it has the same methylation pattern as the E. coli genome 
D)  not recognized as foreign because of the phage restriction and modification system.

Question 2

Dideoxy DNA sequencing is based on chain termination with dideoxynucleotides. Why are normal deoxynucleotides also included in the reaction?

Accepted Answer

A)  to act as a primer for chain termination by the dideoxynucleotides 
B)  to provide a template for DNA polymerase 
C)  to allow production of a range of synthesis products of different lengths. 
D)  to provide a 2?-OH required for incorporation of the dideoxynucleotides 
A)  to act as a primer for chain termination by the dideoxynucleotides 
B)  to provide a template for DNA polymerase 
C)  to allow production of a range of synthesis products of different lengths. 
D)  to provide a 2?-OH required for incorporation of the dideoxynucleotides

Question 3

What are the relative advantages and disadvantages of end-labeling versus internal-labeling a probe?

Accepted Answer

Internal labeling provides maximal signa

Question 4

Recombinant DNA technology became possible with the discovery of which of the following enzymes?

Accepted Answer

A)  terminal deoxynucleotidyl transferase 
B)  restriction endonucleases and DNA ligase 
C)  DNA polymerase and DNA ligase 
D)  reverse transcriptase 
A)  terminal deoxynucleotidyl transferase 
B)  restriction endonucleases and DNA ligase 
C)  DNA polymerase and DNA ligase 
D)  reverse transcriptase

Question 5

Why do foreign DNAs need to be inserted into vectors to clone them?

Accepted Answer

Without a vector, DN

Question 6

In order to function, yeast artificial chromosome (YAC) vectors must include the following:

Accepted Answer

A)  an origin of replication 
B)  a centromere and telomeres 
C)  growth selectable markers 
D)  all of the above 
A)  an origin of replication 
B)  a centromere and telomeres 
C)  growth selectable markers 
D)  all of the above

Question 7

The polymerase chain reaction does not require

Accepted Answer

A)  dNTPs 
B)  DNA ligase 
C)  DNA polymerase 
D)  primers 
A)  dNTPs 
B)  DNA ligase 
C)  DNA polymerase 
D)  primers

Question 8

Complete the incomplete diagrams below to show the key structural difference between an NTP, dNTP, and ddNTP (e.g., CTP, dCTP, ddCTP). 
‪   (a) What do "d" and "dd" stand for?
(b) Explain why ddNTPs are called "chain terminators"
in DNA sequencing reactions.

Accepted Answer

‪ @#IMG-DLM& (a) "d" stands for "deoxy" meaning th

Question 9

The smallest fragment at the end of a DNA sequencing gel represents the

Accepted Answer

A)  2? end of the newly synthesized DNA. 
B)  3? end of the newly synthesized DNA. 
C)  5? end of the newly synthesized DNA. 
D)  either end of newly synthesized DNA, depending on how the sample was loaded on the gel. 
A)  2? end of the newly synthesized DNA. 
B)  3? end of the newly synthesized DNA. 
C)  5? end of the newly synthesized DNA. 
D)  either end of newly synthesized DNA, depending on how the sample was loaded on the gel.

Question 10

In recombinant DNA technology, the term competency refers to

Accepted Answer

A)  the ability of DNA ends to be ligated 
B)  the ability of an enzyme to be activated by ATP 
C)  the ability of cells to take up foreign DNA 
D)  the ability of a medium to select for transformed cells 
A)  the ability of DNA ends to be ligated 
B)  the ability of an enzyme to be activated by ATP 
C)  the ability of cells to take up foreign DNA 
D)  the ability of a medium to select for transformed cells

Question 11

Pedigree analysis was used to determine whether a genetic marker is inherited with a disease trait (black symbols). Results of Southern blot analysis of restriction endonuclease-digested DNA from members of the family are shown. What conclusions can be drawn from the data?

Accepted Answer

A)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal recessivetrait. 
B)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal dominanttrait. 
C)  The disease is caused by expansion of a trinucleotide repeat. 
D)  No information can be gained from this analysis because researchers should haveperformed a Northern blot, not a Southern blot. 
A)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal recessivetrait. 
B)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal dominanttrait. 
C)  The disease is caused by expansion of a trinucleotide repeat. 
D)  No information can be gained from this analysis because researchers should haveperformed a Northern blot, not a Southern blot.

Question 12

Describe the process of cloning a DNA fragment into the multiple cloning site of the vector pUC18. How would you screen for clones that contain an insert?

Accepted Answer

The process of cloning a DNA fragment in

Question 13

Which of the following sequences is in the correct order for one cycle of PCR?

Accepted Answer

A)  Denature DNA; primer extension, anneal primers. 
B)  Primer extension; anneal primers, denature DNA. 
C)  Anneal primers; denature DNA; primer extension. 
D)  Denature DNA; anneal primers; primer extension. 
A)  Denature DNA; primer extension, anneal primers. 
B)  Primer extension; anneal primers, denature DNA. 
C)  Anneal primers; denature DNA; primer extension. 
D)  Denature DNA; anneal primers; primer extension.

Question 14

Which diagram (I or II) shows the correct drawing of gel electrophoresis of DNA fragments of differing size? ?

Accepted Answer

A)  I 
B)  II 
A)  I 
B)  II

Question 15

Phosphorus-32 (32P) is commonly used for labeling

Accepted Answer

A)  dNTPs and NTPs 
B)  amino acids 
C)  nitrogenous bases 
D)  chloramphenicol 
A)  dNTPs and NTPs 
B)  amino acids 
C)  nitrogenous bases 
D)  chloramphenicol

Question 16

Why is E. coli so widely used as a host organism in which to clone genes?

Accepted Answer

A)  E. coli has a rapid growth rate. 
B)  Growing cultures of E. coli cells is relatively easy. 
C)  Many different E. coli strains with distinct and useful properties are available. 
D)  All of the above 
A)  E. coli has a rapid growth rate. 
B)  Growing cultures of E. coli cells is relatively easy. 
C)  Many different E. coli strains with distinct and useful properties are available. 
D)  All of the above

Question 17

Explain the underlying principle between blue-white colony screening.

Accepted Answer

Blue-white colony screening is a techniq

Question 18

Pedigree analysis was used to determine whether a genetic marker is inherited with a disease trait (black circle). Results of Southern blot analysis of restriction endonuclease-digested DNA from members of the family are shown. What conclusions can be drawn from the data?

Accepted Answer

A)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal recessivetrait. 
B)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal dominanttrait. 
C)  The disease is caused by expansion of a trinucleotide repeat. 
D)  No information can be gained from this analysis because researchers should haveperformed a Northern blot, not a Southern blot. 
A)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal recessivetrait. 
B)  A restriction fragment length polymorphism (RFLP) is linked to this autosomal dominanttrait. 
C)  The disease is caused by expansion of a trinucleotide repeat. 
D)  No information can be gained from this analysis because researchers should haveperformed a Northern blot, not a Southern blot.

Question 19

Compare and contrast the key characteristics of automated sequencing using the Sanger method and 454 pyrosequencing.

Accepted Answer

Automated sequencing using the Sanger me

Question 20

When restriction endonucleases first bind to DNA they random walk, slide, hop, and jump. Why don't they immediately get on with their business of cutting DNA?

Accepted Answer

Restriction endonucleases are enzymes th

When E. coli host strain K-12 is infected by phage lambda K, the phage DNA is

Dideoxy DNA sequencing is based on chain termination with dideoxynucleotides. Why are normal deoxynucleotides also included in the reaction?

What are the relative advantages and disadvantages of end-labeling versus internal-labeling a probe?

Recombinant DNA technology became possible with the discovery of which of the following enzymes?

Why do foreign DNAs need to be inserted into vectors to clone them?

In order to function, yeast artificial chromosome (YAC) vectors must include the following:

The polymerase chain reaction does not require

Complete the incomplete diagrams below to show the key structural difference between an NTP, dNTP, and ddNTP (e.g., CTP, dCTP, ddCTP). ‪ (a) What do "d" and "dd" stand for? (b) Explain why ddNTPs are called "chain terminators" in DNA sequencing reactions.

The smallest fragment at the end of a DNA sequencing gel represents the

In recombinant DNA technology, the term competency refers to

Pedigree analysis was used to determine whether a genetic marker is inherited with a disease trait (black symbols). Results of Southern blot analysis of restriction endonuclease-digested DNA from members of the family are shown. What conclusions can be drawn from the data?

Describe the process of cloning a DNA fragment into the multiple cloning site of the vector pUC18. How would you screen for clones that contain an insert?

Which of the following sequences is in the correct order for one cycle of PCR?

Which diagram (I or II) shows the correct drawing of gel electrophoresis of DNA fragments of differing size? ?

Phosphorus-32 (³²P) is commonly used for labeling

Why is E. coli so widely used as a host organism in which to clone genes?

Explain the underlying principle between blue-white colony screening.

Pedigree analysis was used to determine whether a genetic marker is inherited with a disease trait (black circle). Results of Southern blot analysis of restriction endonuclease-digested DNA from members of the family are shown. What conclusions can be drawn from the data?

Compare and contrast the key characteristics of automated sequencing using the Sanger method and 454 pyrosequencing.

When restriction endonucleases first bind to DNA they random walk, slide, hop, and jump. Why don't they immediately get on with their business of cutting DNA?

The Beginnings of Molecular Biology

The Structure of DNA

The Versatility of RNA

Protein Structure and Folding

Genome Organization and Evolution

DNA Replication and Telomere Maintenance

DNA Repair Pathways

Tools for Analyzing Gene Expression

Transcription in Bacteria

Transcription in Eukaryotes

Epigenetic Mechanisms of Gene Regulation

RNA Processing and Post-Transcriptional Gene Regulation

The Mechanism of Translation

Genetically Modified Organisms: Use in Basic and Applied Research

Genome Analysis: DNA Typing, Genomics, and Beyond

Medical Molecular Biology

Filters

Exam 8: Recombinant Dna Technology and Molecular Cloning

When E. coli host strain K-12 is infected by phage lambda K, the phage DNA is

Dideoxy DNA sequencing is based on chain termination with dideoxynucleotides. Why are normal deoxynucleotides also included in the reaction?

What are the relative advantages and disadvantages of end-labeling versus internal-labeling a probe?

Recombinant DNA technology became possible with the discovery of which of the following enzymes?

Why do foreign DNAs need to be inserted into vectors to clone them?

In order to function, yeast artificial chromosome (YAC) vectors must include the following:

The polymerase chain reaction does not require

Complete the incomplete diagrams below to show the key structural difference between an NTP, dNTP, and ddNTP (e.g., CTP, dCTP, ddCTP). ‪ (a) What do "d" and "dd" stand for? (b) Explain why ddNTPs are called "chain terminators" in DNA sequencing reactions.

The smallest fragment at the end of a DNA sequencing gel represents the

In recombinant DNA technology, the term competency refers to

Pedigree analysis was used to determine whether a genetic marker is inherited with a disease trait (black symbols). Results of Southern blot analysis of restriction endonuclease-digested DNA from members of the family are shown. What conclusions can be drawn from the data?

Describe the process of cloning a DNA fragment into the multiple cloning site of the vector pUC18. How would you screen for clones that contain an insert?

Which of the following sequences is in the correct order for one cycle of PCR?

Which diagram (I or II) shows the correct drawing of gel electrophoresis of DNA fragments of differing size? ?

Phosphorus-32 (32P) is commonly used for labeling

Why is E. coli so widely used as a host organism in which to clone genes?

Explain the underlying principle between blue-white colony screening.

Pedigree analysis was used to determine whether a genetic marker is inherited with a disease trait (black circle). Results of Southern blot analysis of restriction endonuclease-digested DNA from members of the family are shown. What conclusions can be drawn from the data?

Compare and contrast the key characteristics of automated sequencing using the Sanger method and 454 pyrosequencing.

When restriction endonucleases first bind to DNA they random walk, slide, hop, and jump. Why don't they immediately get on with their business of cutting DNA?

The Beginnings of Molecular Biology

The Structure of DNA

The Versatility of RNA

Protein Structure and Folding

Genome Organization and Evolution

DNA Replication and Telomere Maintenance

DNA Repair Pathways

Tools for Analyzing Gene Expression

Transcription in Bacteria

Transcription in Eukaryotes

Epigenetic Mechanisms of Gene Regulation

RNA Processing and Post-Transcriptional Gene Regulation

The Mechanism of Translation

Genetically Modified Organisms: Use in Basic and Applied Research

Genome Analysis: DNA Typing, Genomics, and Beyond

Medical Molecular Biology

Filters

Phosphorus-32 (³²P) is commonly used for labeling