Question 1

The eukaryotic TATA-binding protein (TBP) functions in a manner similar to

Accepted Answer

A) sigma (s) factor in E.coli. 
B) rho (r) factor in E.coli. 
C) lac I in E.coli. 
D) CAP in E.coli. 
A) sigma (s) factor in E.coli. 
B) rho (r) factor in E.coli. 
C) lac I in E.coli. 
D) CAP in E.coli.

Question 2

Which of the conditions would result in the least amount of transcription of the lac operon?

Accepted Answer

A)  I 
B)  II 
C)  III 
D)  IV 
A)  I 
B)  II 
C)  III 
D)  IV

Question 3

DNA binding domains are distinct from transcription-activation domains.

Accepted Answer

A) True 
 B)False

Question 4

Ribozymes,the catalytic activity of RNA,were first discovered as part of the snRNA group.

Accepted Answer

A) True 
 B)False

Question 5

CREB contains the following structural motif:

Accepted Answer

A)  Helix-turn-helix 
B)  b-barrel 
C)  Zinc finger 
D)  Leucine zipper 
A)  Helix-turn-helix 
B)  b-barrel 
C)  Zinc finger 
D)  Leucine zipper

Question 6

Where is the TATA box located?

Accepted Answer

A)  At the transcription start site (+1). 
B)  -10 region. 
C)  -25 region. 
D)  -40 region. 
A)  At the transcription start site (+1). 
B)  -10 region. 
C)  -25 region. 
D)  -40 region.

Question 7

What provides the energy for rho-dependent chain termination?

Accepted Answer

A)  ATP hydrolysis distinct from any incorporation into the chain. 
B)  Nucleotide hydrolysis associated with incorporation into the chain. 
C)  Torsional stress built into the separating DNA strands. 
D)  There is no energy requirement. 
A)  ATP hydrolysis distinct from any incorporation into the chain. 
B)  Nucleotide hydrolysis associated with incorporation into the chain. 
C)  Torsional stress built into the separating DNA strands. 
D)  There is no energy requirement.

Question 8

The end of the new mRNA molecule in E.coli.usually terminates in a string of A's

Accepted Answer

A) True 
 B)False

Question 9

Post-transcription processing of mRNA in prokaryotes is required.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following best describes the order of events at the promoter?

Accepted Answer

A)  open complex closed complex transcription initiation 
B)  closed complex open complex transcription initiation 
C)  open complex transcription initiation closed complex 
D)  transcription initiation open complex closed complex 
A)  open complex closed complex transcription initiation 
B)  closed complex open complex transcription initiation 
C)  open complex transcription initiation closed complex 
D)  transcription initiation open complex closed complex

Question 11

The promoter site is

Accepted Answer

A)  the start site for transcription in DNA 
B)  the binding site for regulatory proteins that stimulate transcription 
C)  the general region of DNA downstream from the start site 
D)  the site on DNA at which RNA polymerase binds to initiate transcription 
A)  the start site for transcription in DNA 
B)  the binding site for regulatory proteins that stimulate transcription 
C)  the general region of DNA downstream from the start site 
D)  the site on DNA at which RNA polymerase binds to initiate transcription

Question 12

Splicing reactions for exons involve the following mechanisms,except:

Accepted Answer

A)  Small nuclear RNAs 
B)  Ribozymes 
C)  Lariat mechanisms 
D)  Participation of special G residues 
E)  All of these are involved in splicing reactions 
A)  Small nuclear RNAs 
B)  Ribozymes 
C)  Lariat mechanisms 
D)  Participation of special G residues 
E)  All of these are involved in splicing reactions

Question 13

How do eukaryotic and prokaryotic RNA polymerases compare?

Accepted Answer

A)  Since eukaryotic RNA polymerases are more complex, little homology has been found between the actual protein sequences in the catalytic subunits. 
B)  Since eukaryotic transcription is less complex than in prokaryotes, monomeric RNA polymerases are used. 
C)  Despite their added complexity, eukaryotic and prokaryotic RNA polymerases are generally homologous. 
D)  Eukaryotic and prokaryotic RNA polymerases are virtually identical. 
A)  Since eukaryotic RNA polymerases are more complex, little homology has been found between the actual protein sequences in the catalytic subunits. 
B)  Since eukaryotic transcription is less complex than in prokaryotes, monomeric RNA polymerases are used. 
C)  Despite their added complexity, eukaryotic and prokaryotic RNA polymerases are generally homologous. 
D)  Eukaryotic and prokaryotic RNA polymerases are virtually identical.

Question 14

Which enzyme transcribes genes encoding tRNA in eukaryotes?

Accepted Answer

A)  RNA polymerase I 
B)  RNA polymerase II 
C)  RNA polymerase III 
D)  Different tRNAs are transcribed by different RNA polymerases. 
A)  RNA polymerase I 
B)  RNA polymerase II 
C)  RNA polymerase III 
D)  Different tRNAs are transcribed by different RNA polymerases.

Question 15

The weakness of A-U base pairs at the end of the RNA molecule may help in dissociation of the new RNA product.

Accepted Answer

A) True 
 B)False

Question 16

Which of these terms does not describe the DNA strand used to direct RNA synthesis?

Accepted Answer

A)  Template strand 
B)  Coding strand 
C)  Antisense strand 
D)  Negative or "-" strand 
E)  All these terms describe the complementary DNA strand 
A)  Template strand 
B)  Coding strand 
C)  Antisense strand 
D)  Negative or "-" strand 
E)  All these terms describe the complementary DNA strand

Question 17

Which of following statements concerning RNA transcription is false?

Accepted Answer

A)  The DNA strands become separated during synthesis. 
B)  Synthesis of RNA is as accurate as synthesis of DNA 
C)  The template strand is read in the 3' 5' direction. 
D)  All 4 ribonucleotides are required. 
A)  The DNA strands become separated during synthesis. 
B)  Synthesis of RNA is as accurate as synthesis of DNA 
C)  The template strand is read in the 3' 5' direction. 
D)  All 4 ribonucleotides are required.

Question 18

There is only one sigma subunit,since the same RNA Polymerase must bind to all genes in E.coli.

Accepted Answer

A) True 
 B)False

Question 19

The sequences in eukaryotic DNA known as introns are

Accepted Answer

A)  those included in the final sequence of messenger RNA 
B)  the intervening sequences not expressed in the final sequence of messenger RNA 
C)  the binding sites for DNA polymerase 
D)  the binding sites for RNA polymerase 
A)  those included in the final sequence of messenger RNA 
B)  the intervening sequences not expressed in the final sequence of messenger RNA 
C)  the binding sites for DNA polymerase 
D)  the binding sites for RNA polymerase

Question 20

The lariat mechanism has been demonstrated by observing the lariat structure using electron microscopy.

Accepted Answer

A) True 
 B)False

Exam 11: Transcription of the Genetic Code: Biosynthesis of Rna

The eukaryotic TATA-binding protein (TBP) functions in a manner similar to

Which of the conditions would result in the least amount of transcription of the lac operon?

DNA binding domains are distinct from transcription-activation domains.

Ribozymes,the catalytic activity of RNA,were first discovered as part of the snRNA group.

CREB contains the following structural motif:

Where is the TATA box located?

What provides the energy for rho-dependent chain termination?

The end of the new mRNA molecule in E.coli.usually terminates in a string of A's

Post-transcription processing of mRNA in prokaryotes is required.

Which of the following best describes the order of events at the promoter?

The promoter site is

Splicing reactions for exons involve the following mechanisms,except:

How do eukaryotic and prokaryotic RNA polymerases compare?

Which enzyme transcribes genes encoding tRNA in eukaryotes?

The weakness of A-U base pairs at the end of the RNA molecule may help in dissociation of the new RNA product.

Which of these terms does not describe the DNA strand used to direct RNA synthesis?

Which of following statements concerning RNA transcription is false?

There is only one sigma subunit,since the same RNA Polymerase must bind to all genes in E.coli.

The sequences in eukaryotic DNA known as introns are

The lariat mechanism has been demonstrated by observing the lariat structure using electron microscopy.

Biochemistry and the Organization of Cells

Water: The Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Protein Synthesis: Translation of the Genetic Message

Nucleic Acid Biotechnology Techniques

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

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