Question 1

Operons

Accepted Answer

A)  control the expression of constitutive genes. 
B)  are subject to positive or to negative control. 
C)  are not affected by mutations in the genes for repressors or inducers. 
D)  occur in both prokaryotes and eukaryotes. 
A)  control the expression of constitutive genes. 
B)  are subject to positive or to negative control. 
C)  are not affected by mutations in the genes for repressors or inducers. 
D)  occur in both prokaryotes and eukaryotes.

Question 2

Inducers allow for a system in which an enzyme is not made unless it is needed.

Accepted Answer

A) True 
 B)False

Question 3

Intracellular levels of cyclic AMP can affect the phosphorylation of proteins and enzymes as a secondary messenger.

Accepted Answer

A) True 
 B)False

Question 4

What is the need for a primer strand in transcription?

Accepted Answer

A)  It ensures the fidelity of the newly synthesized RNA strand. 
B)  There is none. 
C)  RNA polymerases requires a preexisting strand with a nucleotide having a 3'-OH. 
D)  RNA polymerase requires a preexisting strand with a nucleotide having a 5'-OH. 
A)  It ensures the fidelity of the newly synthesized RNA strand. 
B)  There is none. 
C)  RNA polymerases requires a preexisting strand with a nucleotide having a 3'-OH. 
D)  RNA polymerase requires a preexisting strand with a nucleotide having a 5'-OH.

Question 5

Polyadenylation of eukaryotic mRNA

Accepted Answer

A)  occurs at the 5' end. 
B)  occurs at the 3' end. 
C)  occurs at both ends. 
D)  doesn't occur at all. 
A)  occurs at the 5' end. 
B)  occurs at the 3' end. 
C)  occurs at both ends. 
D)  doesn't occur at all.

Question 6

Phosphorylation of the CTD of RNA polymerase II occurs during which phase of transcription?

Accepted Answer

A)  initial binding to the promoter 
B)  conversion from the closed complex to the open complex 
C)  termination of transcription 
D)  None of these 
A)  initial binding to the promoter 
B)  conversion from the closed complex to the open complex 
C)  termination of transcription 
D)  None of these

Question 7

Which of the following influence the amount of expression of an operon?

Accepted Answer

A)  Availability of the specific sigma factor for that operon. 
B)  How well the Pribnow box conforms to the consensus sequence. 
C)  Attenuation mechanisms. 
D)  Presence of 3' 5' cyclic AMP. 
E)  All of these. 
A)  Availability of the specific sigma factor for that operon. 
B)  How well the Pribnow box conforms to the consensus sequence. 
C)  Attenuation mechanisms. 
D)  Presence of 3' 5' cyclic AMP. 
E)  All of these.

Question 8

Which of the following is not a characteristic of catabolite activator protein (CAP)?

Accepted Answer

A)  it is a positive regulator of the lac operon 
B)  when the cell has sufficient glucose and lactose, CAP will not be bound to the CAP binding site 
C)  CAP binding near the promoter site depends on CAP complexation with cAMP 
D)  the binding of CAP to DNA requires ATP hydrolysis 
A)  it is a positive regulator of the lac operon 
B)  when the cell has sufficient glucose and lactose, CAP will not be bound to the CAP binding site 
C)  CAP binding near the promoter site depends on CAP complexation with cAMP 
D)  the binding of CAP to DNA requires ATP hydrolysis

Question 9

RNA synthesis begins at the base in the DNA sequence designated by the following number:

Accepted Answer

A)  +1 (plus one) 
B)  0 (zero) 
C)  -1 (minus one) 
D)  -10 region (minus ten) 
E)  It varies among genes. 
A)  +1 (plus one) 
B)  0 (zero) 
C)  -1 (minus one) 
D)  -10 region (minus ten) 
E)  It varies among genes.

Question 10

Alternative splicing of exons is a mechanism to get more than 1 protein from the same gene.

Accepted Answer

A) True 
 B)False

Question 11

Chain termination occurs,in vivo,when:

Accepted Answer

A)  RNA Pol gets to the end of the DNA. 
B)  The factor called rho (r) binds to the DNA. 
C)  A hairpin loop forms in the template. 
D)  Either a hairpin loop forms or rho (r) is involved. 
A)  RNA Pol gets to the end of the DNA. 
B)  The factor called rho (r) binds to the DNA. 
C)  A hairpin loop forms in the template. 
D)  Either a hairpin loop forms or rho (r) is involved.

Question 12

Which category of ribozymes requires an external guanosine frothier action?

Accepted Answer

A)  Group I 
B)  Group II 
C)  Both Group I & Group II 
D)  Neither Group I nor Group II 
A)  Group I 
B)  Group II 
C)  Both Group I & Group II 
D)  Neither Group I nor Group II

Question 13

The catabolite activator protein can overcome the effect of a repressor binding to the operon.

Accepted Answer

A) True 
 B)False

Question 14

Which of following statements concerning RNA transcription is false?

Accepted Answer

A)  The release of pyrophosphate from a nucleoside triphosphate drives the reaction. 
B)  RNA is synthesized from the 5' end to the 3' end. 
C)  DNA to RNA base pairing includes A to U and G to C 
D)  Transcription requires the use of a primer. 
A)  The release of pyrophosphate from a nucleoside triphosphate drives the reaction. 
B)  RNA is synthesized from the 5' end to the 3' end. 
C)  DNA to RNA base pairing includes A to U and G to C 
D)  Transcription requires the use of a primer.

Question 15

TATA-binding protein (TBP) is required for transcription by

Accepted Answer

A)  Pol I. 
B)  Pol II. 
C)  Pol III. 
D)  all of these polymerases. 
A)  Pol I. 
B)  Pol II. 
C)  Pol III. 
D)  all of these polymerases.

Question 16

Which of the following is not a common modification occurring after polymerization of RNA?

Accepted Answer

A)  Methylation of bases. 
B)  Addition of phosphate to the bases. 
C)  Removal of bases from the polymer. 
D)  Addition of bases to the polymer. 
E)  All of these changes are common. 
A)  Methylation of bases. 
B)  Addition of phosphate to the bases. 
C)  Removal of bases from the polymer. 
D)  Addition of bases to the polymer. 
E)  All of these changes are common.

Question 17

Which of the conditions would result in the greatest amount of transcription of the lac operon?

Accepted Answer

A)  I 
B)  II 
C)  III 
D)  IV 
A)  I 
B)  II 
C)  III 
D)  IV

Question 18

RNA polymerases from prokaryotes and eukaryotes

Accepted Answer

A)  have sequence homology in catalytic subunits 
B)  have identical s factors 
C)  differ because there is no analogue to the prokaryotic a subunit in eukaryotes 
D)  have the same number and kind of subunits 
A)  have sequence homology in catalytic subunits 
B)  have identical s factors 
C)  differ because there is no analogue to the prokaryotic a subunit in eukaryotes 
D)  have the same number and kind of subunits

Question 19

The leucine zipper is different from other DNA binding domains,since it requires the DNA-binding protein to form a dimer.

Accepted Answer

A) True 
 B)False

Question 20

Capping of eukaryotic mRNA

Accepted Answer

A)  occurs at the 5' end. 
B)  occurs at the 3' end. 
C)  occurs at both ends. 
D)  doesn't occur at all. 
A)  occurs at the 5' end. 
B)  occurs at the 3' end. 
C)  occurs at both ends. 
D)  doesn't occur at all.

Exam 11: Transcription of the Genetic Code: Biosynthesis of Rna

Operons

Inducers allow for a system in which an enzyme is not made unless it is needed.

Intracellular levels of cyclic AMP can affect the phosphorylation of proteins and enzymes as a secondary messenger.

What is the need for a primer strand in transcription?

Polyadenylation of eukaryotic mRNA

Phosphorylation of the CTD of RNA polymerase II occurs during which phase of transcription?

Which of the following influence the amount of expression of an operon?

Which of the following is not a characteristic of catabolite activator protein (CAP)?

RNA synthesis begins at the base in the DNA sequence designated by the following number:

Alternative splicing of exons is a mechanism to get more than 1 protein from the same gene.

Chain termination occurs,in vivo,when:

Which category of ribozymes requires an external guanosine frothier action?

The catabolite activator protein can overcome the effect of a repressor binding to the operon.

Which of following statements concerning RNA transcription is false?

TATA-binding protein (TBP) is required for transcription by

Which of the following is not a common modification occurring after polymerization of RNA?

Which of the conditions would result in the greatest amount of transcription of the lac operon?

RNA polymerases from prokaryotes and eukaryotes

The leucine zipper is different from other DNA binding domains,since it requires the DNA-binding protein to form a dimer.

Capping of eukaryotic mRNA

Biochemistry and the Organization of Cells

Water: The Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Protein Synthesis: Translation of the Genetic Message

Nucleic Acid Biotechnology Techniques

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

Filters