Question 1

In E.coli,

Accepted Answer

A)  the leading strand is synthesized in one piece while the lagging strand is synthesized discontinuously. 
B)  the leading strand is synthesized discontinuously while the lagging strand is synthesized in one piece. 
C)  both the leading and lagging strands are synthesized in one piece. 
D)  both the leading and lagging strands are synthesized discontinuously. 
A)  the leading strand is synthesized in one piece while the lagging strand is synthesized discontinuously. 
B)  the leading strand is synthesized discontinuously while the lagging strand is synthesized in one piece. 
C)  both the leading and lagging strands are synthesized in one piece. 
D)  both the leading and lagging strands are synthesized discontinuously.

Question 2

Which of the following activities does

Accepted Answer

A) 5' 3' polymerase 
B) 5' 3' exonuclease 
C) 3' 5' exonuclease 
D) E.coli DNA polymerase III has ALL of the above activities. 
A) 5' 3' polymerase 
B) 5' 3' exonuclease 
C) 3' 5' exonuclease 
D) E.coli DNA polymerase III has ALL of the above activities.

Question 3

Semiconservative replication of DNA was established experimentally by

Accepted Answer

A)  gel electrophoresis. 
B)  ultraviolet spectroscopy. 
C)  column chromatography. 
D)  density-gradient centrifugation. 
A)  gel electrophoresis. 
B)  ultraviolet spectroscopy. 
C)  column chromatography. 
D)  density-gradient centrifugation.

Question 4

The enzyme telomerase uses mechanisms that involve:

Accepted Answer

A)  Repeating sequences at the telomeres. 
B)  Having RNA oligonucleotides to act as templates as part of the enzyme. 
C)  Allowing the end of the chromosome to get a little shorter each time a cell divides. 
D)  Both repeating sequences at the end of chromosomes and RNA oligonucleotides to act as templates as part of the enzyme. 
E)  All of these. 
A)  Repeating sequences at the telomeres. 
B)  Having RNA oligonucleotides to act as templates as part of the enzyme. 
C)  Allowing the end of the chromosome to get a little shorter each time a cell divides. 
D)  Both repeating sequences at the end of chromosomes and RNA oligonucleotides to act as templates as part of the enzyme. 
E)  All of these.

Question 5

Which of the following is a characteristic of eukaryotic,but not prokaryotic,DNA replication?

Accepted Answer

A)  Topoisomerases are required. 
B)  A primer is needed on the lagging strand only. 
C)  Histone biosynthesis must take place. 
D)  There is only one origin of replication. 
A)  Topoisomerases are required. 
B)  A primer is needed on the lagging strand only. 
C)  Histone biosynthesis must take place. 
D)  There is only one origin of replication.

Question 6

Telomerase activity in cancer cells may explain their longevity.

Accepted Answer

A) True 
 B)False

Question 7

The flow of genetic information is RNA DNA in

Accepted Answer

A)  all organisms. 
B)  all prokaryotes. 
C)  retroviruses. 
D)  no known organisms. 
A)  all organisms. 
B)  all prokaryotes. 
C)  retroviruses. 
D)  no known organisms.

Question 8

In Escherichia coli,the enzyme principally responsible for the synthesis of new DNA strands is

Accepted Answer

A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  primase 
A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  primase

Question 9

E.coli replication on the lagging strand

Accepted Answer

A)  is carried out by DNA polymerase I 
B)  is initially synthesized as Okazaki fragments 
C)  is synthesized continuously 
D)  has this DNA strand synthesized in a 3' 5' direction 
A)  is carried out by DNA polymerase I 
B)  is initially synthesized as Okazaki fragments 
C)  is synthesized continuously 
D)  has this DNA strand synthesized in a 3' 5' direction

Question 10

Which of the following is not a function of DNA Polymerase III?

Accepted Answer

A)  Polymerization. 
B)  Ligating the final products. 
C)  Proofreading. 
D)  Clamping on to the template. 
E)  All of these. 
A)  Polymerization. 
B)  Ligating the final products. 
C)  Proofreading. 
D)  Clamping on to the template. 
E)  All of these.

Question 11

All the following describe the general mechanism of DNA synthesis,except:

Accepted Answer

A)  From the perspective of the DNA template strands, one strand is made 5' 3' while the other strand is made 3' 5'. 
B)  The strands become separated during synthesis. 
C)  Synthesis occurs in both directions from the starting site of synthesis. 
D)  Synthesis of DNA is a very accurate process. 
E)  All of these are correct. 
A)  From the perspective of the DNA template strands, one strand is made 5' 3' while the other strand is made 3' 5'. 
B)  The strands become separated during synthesis. 
C)  Synthesis occurs in both directions from the starting site of synthesis. 
D)  Synthesis of DNA is a very accurate process. 
E)  All of these are correct.

Question 12

The process by which an mRNA base sequence directs the amino acid sequence of a protein is called

Accepted Answer

A)  replication. 
B)  transcription. 
C)  translation. 
D)  nucleation. 
A)  replication. 
B)  transcription. 
C)  translation. 
D)  nucleation.

Question 13

Suppose Meselson & Stahl had observed the following data in their famous experiment involving a switch from medium containing 15N to 14N.What would they have concluded about the nature of DNA replication?

Accepted Answer

A)  They still would have concluded that DNA replication is semiconservative, i.e., each double helix contains one parental strand and one newly synthesized strand. 
B)  They would have concluded that DNA replication is conservative, i.e., one double helix contains only the parental strands, while the other contains only newly synthesized DNA. 
C)  They would have concluded that DNA replication is dispersive/random, i.e., each strand of each double helix contains a mixture of parental and newly synthesized sequences. 
D)  They would have been unable to distinguish which is correct. 
A)  They still would have concluded that DNA replication is semiconservative, i.e., each double helix contains one parental strand and one newly synthesized strand. 
B)  They would have concluded that DNA replication is conservative, i.e., one double helix contains only the parental strands, while the other contains only newly synthesized DNA. 
C)  They would have concluded that DNA replication is dispersive/random, i.e., each strand of each double helix contains a mixture of parental and newly synthesized sequences. 
D)  They would have been unable to distinguish which is correct.

Question 14

The mechanism of breakage and reunion of DNA strands during recombination was proposed by:

Accepted Answer

A)  Messelson and Stahl 
B)  Messelson and Weigle 
C)  Robin Holliday 
D)  Francis Crick 
A)  Messelson and Stahl 
B)  Messelson and Weigle 
C)  Robin Holliday 
D)  Francis Crick

Question 15

Exhibit 10A   Consider the following diagrams showing a replication fork moving from left to right.The thick lines represent the template/parental strands.The 5' and 3' represent the ends of those template/parental strands.
Refer to Exhibit 10A.Which diagram correctly depicts the orientation of the lagging and leading strands on the parentals?

Accepted Answer

A)  The top 
B)  The bottom 
C)  Neither is fully accurate. 
D)  Either would be accurate dependent on the organism being studied. 
A)  The top 
B)  The bottom 
C)  Neither is fully accurate. 
D)  Either would be accurate dependent on the organism being studied.

Question 16

Eukaryotic DNA polymerases differ from those of prokaryotes in that

Accepted Answer

A)  they do not require a primer 
B)  they do not always have exonuclease activity 
C)  there is no equivalent to the sliding clamp in prokaryotic replication 
D)  they produce longer Okazaki fragments 
A)  they do not require a primer 
B)  they do not always have exonuclease activity 
C)  there is no equivalent to the sliding clamp in prokaryotic replication 
D)  they produce longer Okazaki fragments

Question 17

The DNA polymerases in eukaryotes have similar functions to those found in bacteria,but they are not identical.

Accepted Answer

A) True 
 B)False

Question 18

Since DNA Polymerase II has endonuclease activity,it is able to proofread its product when it is used in DNA repair.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following characteristics is not associated with

Accepted Answer

A)  it synthesizes the RNA primer in DNA replication 
B)  it synthesizes a primer with a free 3'-OH end 
C)  it is essential for DNA replication 
D)  it is essential for RNA replication 
E)  coli primase? 
A)  it synthesizes the RNA primer in DNA replication 
B)  it synthesizes a primer with a free 3'-OH end 
C)  it is essential for DNA replication 
D)  it is essential for RNA replication 
E)  coli primase?

Question 20

Both eukaryotes and prokaryotes use a dimeric DNA Polymerase for DNA replication.

Accepted Answer

A) True 
 B)False

Exam 10: Biosynthesis of Nucleic Acids: Replication

In E.coli,

Which of the following activities does

Semiconservative replication of DNA was established experimentally by

The enzyme telomerase uses mechanisms that involve:

Which of the following is a characteristic of eukaryotic,but not prokaryotic,DNA replication?

Telomerase activity in cancer cells may explain their longevity.

The flow of genetic information is RNA DNA in

In Escherichia coli,the enzyme principally responsible for the synthesis of new DNA strands is

E.coli replication on the lagging strand

Which of the following is not a function of DNA Polymerase III?

All the following describe the general mechanism of DNA synthesis,except:

The process by which an mRNA base sequence directs the amino acid sequence of a protein is called

Suppose Meselson & Stahl had observed the following data in their famous experiment involving a switch from medium containing 15N to 14N.What would they have concluded about the nature of DNA replication?

The mechanism of breakage and reunion of DNA strands during recombination was proposed by:

Eukaryotic DNA polymerases differ from those of prokaryotes in that

The DNA polymerases in eukaryotes have similar functions to those found in bacteria,but they are not identical.

Since DNA Polymerase II has endonuclease activity,it is able to proofread its product when it is used in DNA repair.

Which of the following characteristics is not associated with

Both eukaryotes and prokaryotes use a dimeric DNA Polymerase for DNA replication.

Biochemistry and the Organization of Cells

Water: The Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Transcription of the Genetic Code: Biosynthesis of Rna

Protein Synthesis: Translation of the Genetic Message

Nucleic Acid Biotechnology Techniques

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

Filters

Suppose Meselson & Stahl had observed the following data in their famous experiment involving a switch from medium containing ¹⁵N to ¹⁴N.What would they have concluded about the nature of DNA replication?