Question 1

Which of the following is essential for the development of discrete, isolated colonies?

Accepted Answer

A) Solid medium 
B) Differential medium 
C) Broth medium 
D) Assay medium 
E) Selective medium 
A) Solid medium 
B) Differential medium 
C) Broth medium 
D) Assay medium 
E) Selective medium A

Question 2

A reducing medium contains

Accepted Answer

A) sugars that can be fermented. 
B) extra oxygen. 
C) hemoglobin, vitamins, or other growth factors. 
D) substances that remove oxygen. 
E) inhibiting agents. 
A) sugars that can be fermented. 
B) extra oxygen. 
C) hemoglobin, vitamins, or other growth factors. 
D) substances that remove oxygen. 
E) inhibiting agents. D

Question 3

The three physical forms of laboratory media are ______.

Accepted Answer

A) solid, semisolid, and liquid 
B) reducing, transport, and enumeration 
C) a streak plate, a pour plate, and a broth 
D) solid, liquid, and gas 
A) solid, semisolid, and liquid 
B) reducing, transport, and enumeration 
C) a streak plate, a pour plate, and a broth 
D) solid, liquid, and gas A

Question 4

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Accepted Answer

A) dark-field 
B) confocal 
C) fluorescence 
D) phase-contrast 
E) electron 
A) dark-field 
B) confocal 
C) fluorescence 
D) phase-contrast 
E) electron

Question 5

A microbiology student with a visual disability is viewing a sample of Bacillus endospores in a phase contrast microscope that is linked to an iPad.By "stretching" the image on the iPad screen, the student can increase the apparent size of the endospores.How does this manipulation affect resolution and contrast? (No software is being used to alter the image!)

Accepted Answer

A) Only the magnification can be increased; resolution and contrast are unchanged. 
B) Contrast will improve because the microscope is phase-contrast. 
C) Both magnification and resolution increase; contrast is unchanged. 
D) Resolution and magnification will decrease because the screen is not as good as the human eye. 
A) Only the magnification can be increased; resolution and contrast are unchanged. 
B) Contrast will improve because the microscope is phase-contrast. 
C) Both magnification and resolution increase; contrast is unchanged. 
D) Resolution and magnification will decrease because the screen is not as good as the human eye.

Question 6

Your microscope has a resolving power of 0.15 $\mu$m.The specimen that you will be observing has bacterial cocci the size of 0.1 $\mu$m in diameter.Which of the following statements is correct?

Accepted Answer

A) The cocci will appear much smaller than their actual size because of the low resolution. 
B) The cocci will be fuzzy-looking and blend together. 
C) The cocci will not be visible. 
D) The cocci will be readily visible and distinct. 
A) The cocci will appear much smaller than their actual size because of the low resolution. 
B) The cocci will be fuzzy-looking and blend together. 
C) The cocci will not be visible. 
D) The cocci will be readily visible and distinct.

Question 7

A pure culture contains

Accepted Answer

A) only bacteria. 
B) only one identified species of microorganism. 
C) a variety of species from the same genus. 
D) microbes from a single source. 
A) only bacteria. 
B) only one identified species of microorganism. 
C) a variety of species from the same genus. 
D) microbes from a single source.

Question 8

Bacteria that require special growth factors and complex nutrients are termed ______.

Accepted Answer

A) fermentative 
B) hemolytic 
C) fastidious 
D) aerobic 
E) anaerobic 
A) fermentative 
B) hemolytic 
C) fastidious 
D) aerobic 
E) anaerobic

Question 9

Which type of microscope shows cells against a white background?

Accepted Answer

A) Confocal 
B) Electron 
C) Bright-field 
D) Fluorescence 
E) Phase-contrast 
A) Confocal 
B) Electron 
C) Bright-field 
D) Fluorescence 
E) Phase-contrast

Question 10

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

A) ocular 
B) condenser 
C) stage 
D) nosepiece 
A) ocular 
B) condenser 
C) stage 
D) nosepiece

Question 11

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Accepted Answer

A) enriched 
B) complex 
C) reducing 
D) synthetic 
A) enriched 
B) complex 
C) reducing 
D) synthetic

Question 12

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

Accepted Answer

A) Spread plate 
B) Streak plate 
C) Pour plate 
D) Culture plate 
E) Loop dilution 
A) Spread plate 
B) Streak plate 
C) Pour plate 
D) Culture plate 
E) Loop dilution

Question 13

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 14

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Accepted Answer

A) True 
 B)False

Question 15

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L-cysteine, sodium chloride, sodium thioglycolate, and agar.It is considered a complex medium because

Accepted Answer

A) depending on their oxygen utilization pattern, organisms will grow differently on it. 
B) it has multiple ingredients for a broad range of nutrients. 
C) both yeast extract and pancreatic digest of casein are not chemically defined. 
D) thioglycolate has a carbon-sulfur bond that many microbes cannot break enzymatically. 
A) depending on their oxygen utilization pattern, organisms will grow differently on it. 
B) it has multiple ingredients for a broad range of nutrients. 
C) both yeast extract and pancreatic digest of casein are not chemically defined. 
D) thioglycolate has a carbon-sulfur bond that many microbes cannot break enzymatically.

Question 16

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Accepted Answer

A) Spread plate 
B) Pour plate 
C) Replica plate 
D) Streak plate 
A) Spread plate 
B) Pour plate 
C) Replica plate 
D) Streak plate

Question 17

Which type of microscope does not use light in forming the specimen image?

Accepted Answer

A) Electron 
B) Dark-field 
C) Phase-contrast 
D) Bright-field 
E) Fluorescence 
A) Electron 
B) Dark-field 
C) Phase-contrast 
D) Bright-field 
E) Fluorescence

Question 18

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

Accepted Answer

A) reducing 
B) enriched 
C) complex 
D) defined 
A) reducing 
B) enriched 
C) complex 
D) defined

Question 19

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

Accepted Answer

A) True 
 B)False

Question 20

Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

Accepted Answer

A) the condenser 
B) the ocular 
C) immersion oil 
D) the cover slip 
A) the condenser 
B) the ocular 
C) immersion oil 
D) the cover slip

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Which of the following is essential for the development of discrete, isolated colonies?

A reducing medium contains

The three physical forms of laboratory media are ______.

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Your microscope has a resolving power of 0.15 μ\muμ m.The specimen that you will be observing has bacterial cocci the size of 0.1 μ\muμ m in diameter.Which of the following statements is correct?

A pure culture contains

Bacteria that require special growth factors and complex nutrients are termed ______.

Which type of microscope shows cells against a white background?

The _____ of the microscope holds and allows selection of the objective lenses.

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

A medium that is gel-like has less agar in it compared to a solid medium.

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L-cysteine, sodium chloride, sodium thioglycolate, and agar.It is considered a complex medium because

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Which type of microscope does not use light in forming the specimen image?

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

The Main Themes of Microbiology

The Chemistry of Biology

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Microbial Nutrition, Ecology, and Growth

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering and Recombinant Dna

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

Filters

Your microscope has a resolving power of 0.15 $\mu$ m.The specimen that you will be observing has bacterial cocci the size of 0.1 $\mu$ m in diameter.Which of the following statements is correct?