Question 1

A scanning confocal microscope

Accepted Answer

A) produces specimen images on electron micrographs. 
B) uses dyes that emit visible light when excited by ultraviolet radiation. 
C) uses ultraviolet light to form a specimen image. 
D) scans narrow planes and can build a three-dimensional image of the specimen. 
A) produces specimen images on electron micrographs. 
B) uses dyes that emit visible light when excited by ultraviolet radiation. 
C) uses ultraviolet light to form a specimen image. 
D) scans narrow planes and can build a three-dimensional image of the specimen.

Question 2

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

Accepted Answer

A) 920 $\mu$m 
B) 0.2 $\mu$m 
C) 175 $\mu$m 
D) 57.5 $\mu$m 
E) 4,000 $\mu$m 
A) 920 $\mu$m 
B) 0.2 $\mu$m 
C) 175 $\mu$m 
D) 57.5 $\mu$m 
E) 4,000 $\mu$m

Question 3

The Five I's of studying microorganisms include all of the following except ______.

Accepted Answer

A) identification 
B) isolation 
C) incubation 
D) infection 
E) inoculation 
A) identification 
B) isolation 
C) incubation 
D) infection 
E) inoculation

Question 4

All of the following pertain to the fluorescence microscope except

Accepted Answer

A) it is a type of compound microscope. 
B) it is used to diagnose certain infections. 
C) it uses electrons to produce a specimen image. 
D) it requires the use of dyes like acridine or fluorescein. 
E) it requires an ultraviolet radiation source. 
A) it is a type of compound microscope. 
B) it is used to diagnose certain infections. 
C) it uses electrons to produce a specimen image. 
D) it requires the use of dyes like acridine or fluorescein. 
E) it requires an ultraviolet radiation source.

Question 5

Bacterial cultures are easily identified from their microscopic appearance.

Accepted Answer

A) True 
 B)False

Question 6

Differential media result in which of the following observable characteristics?

Accepted Answer

A) Differently colored colonies 
B) Different media color after incubation 
C) Chemical precipitates 
D) Gas bubbles 
E) All of the choices are correct. 
A) Differently colored colonies 
B) Different media color after incubation 
C) Chemical precipitates 
D) Gas bubbles 
E) All of the choices are correct.

Question 7

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

A) ocular; oil immersion 
B) scanning; objective 
C) ocular; objective 
D) objective; ocular 
A) ocular; oil immersion 
B) scanning; objective 
C) ocular; objective 
D) objective; ocular

Question 8

The specimen preparation technique that is best for viewing cell motility is the ______.

Accepted Answer

A) flagellar stain 
B) Gram stain 
C) negative stain 
D) hanging drop 
E) fixed, stained smear 
A) flagellar stain 
B) Gram stain 
C) negative stain 
D) hanging drop 
E) fixed, stained smear

Question 9

Who invented the first crude microscope by grinding glass?

Accepted Answer

A) Lister 
B) Schultz and Schwann 
C) Leeuwenhoek 
D) Redi 
E) Pasteur 
A) Lister 
B) Schultz and Schwann 
C) Leeuwenhoek 
D) Redi 
E) Pasteur

Question 10

All of the following are examples of different types of microbiological media except ______.

Accepted Answer

A) agar 
B) enriched 
C) broth 
D) Petri dish 
E) semisolid 
A) agar 
B) enriched 
C) broth 
D) Petri dish 
E) semisolid

Question 11

Agar is a complex polysaccharide that comes from a/an ______.

Accepted Answer

A) bacterium 
B) algae 
C) fungus 
D) marine invertebrate 
E) green plant 
A) bacterium 
B) algae 
C) fungus 
D) marine invertebrate 
E) green plant

Question 12

Comparing transmission electron microscopy with scanning electron microscopy, the following statement is true.

Accepted Answer

A) Transmission EM is used for internal detail of cells and subcellular structures. 
B) Transmission EM gives detail of the external architecture of cells. 
C) Transmission EM requires dyes. 
D) Transmission EM can provide good images of bacteria but not viruses. 
A) Transmission EM is used for internal detail of cells and subcellular structures. 
B) Transmission EM gives detail of the external architecture of cells. 
C) Transmission EM requires dyes. 
D) Transmission EM can provide good images of bacteria but not viruses.

Question 13

Fixed smears of specimens are required to perform the Gram stain or the endospore stain.

Accepted Answer

A) True 
 B)False

Question 14

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

Accepted Answer

A)  The culture medium was selective. 
B)  The culture medium was differential. 
C)  The microbiologist used too much inoculum. 
D)  The culture was contaminated. 
E)  The incubation temperature was incorrect. 
A)  The culture medium was selective. 
B)  The culture medium was differential. 
C)  The microbiologist used too much inoculum. 
D)  The culture was contaminated. 
E)  The incubation temperature was incorrect.

A scanning confocal microscope

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

The Five I's of studying microorganisms include all of the following except ______.

All of the following pertain to the fluorescence microscope except

Bacterial cultures are easily identified from their microscopic appearance.

Differential media result in which of the following observable characteristics?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

The specimen preparation technique that is best for viewing cell motility is the ______.

Who invented the first crude microscope by grinding glass?

All of the following are examples of different types of microbiological media except ______.

Agar is a complex polysaccharide that comes from a/an ______.

Comparing transmission electron microscopy with scanning electron microscopy, the following statement is true.

Fixed smears of specimens are required to perform the Gram stain or the endospore stain.

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

The Main Themes of Microbiology

The Chemistry of Biology

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Microbial Nutrition, Ecology, and Growth

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering and Recombinant Dna

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

Filters

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

A scanning confocal microscope

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

The Five I's of studying microorganisms include all of the following except ______.

All of the following pertain to the fluorescence microscope except

Bacterial cultures are easily identified from their microscopic appearance.

Differential media result in which of the following observable characteristics?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

The specimen preparation technique that is best for viewing cell motility is the ______.

Who invented the first crude microscope by grinding glass?

All of the following are examples of different types of microbiological media except ______.

Agar is a complex polysaccharide that comes from a/an ______.

Comparing transmission electron microscopy with scanning electron microscopy, the following statement is true.

Fixed smears of specimens are required to perform the Gram stain or the endospore stain.

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

The Main Themes of Microbiology

The Chemistry of Biology

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Microbial Nutrition, Ecology, and Growth

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering and Recombinant Dna

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

Filters

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.