Question 1

What is the term for a culture made from one isolated colony?

Accepted Answer

A) Axenic 
B) Mixed 
C) Pure 
D) Both axenic and mixed are correct. 
E) Both axenic and pure are correct. 
A) Axenic 
B) Mixed 
C) Pure 
D) Both axenic and mixed are correct. 
E) Both axenic and pure are correct.

Question 2

What do the Gram stain, the acid-fast stain, and the endospore stain have in common?

Accepted Answer

A) They use heat to force the dye into cell structures. 
B) The staining outcome varies with the charge of the cell wall. 
C) They are used on a wet mount of the specimen. 
D) They are differential stains. 
E) They use a negative stain technique. 
A) They use heat to force the dye into cell structures. 
B) The staining outcome varies with the charge of the cell wall. 
C) They are used on a wet mount of the specimen. 
D) They are differential stains. 
E) They use a negative stain technique.

Question 3

Which type of medium would be the best choice when shipping a sample of bacteria from a clinic to a laboratory for testing?

Accepted Answer

A) Thioglycolate medium 
B) Eosin-methylene blue agar 
C) Transport medium 
D) Blood agar 
A) Thioglycolate medium 
B) Eosin-methylene blue agar 
C) Transport medium 
D) Blood agar

Question 4

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Accepted Answer

A) 0.2 mm 
B) 0.1 µm 
C) 2.0 µm 
D) 0.2 µm 
A) 0.2 mm 
B) 0.1 µm 
C) 2.0 µm 
D) 0.2 µm

Question 5

Which type of microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A) Bright-field 
B) Fluorescence 
C) Electron 
D) Dark-field 
E) Phase-contrast 
A) Bright-field 
B) Fluorescence 
C) Electron 
D) Dark-field 
E) Phase-contrast

Question 6

Which type of microscope cannot image live specimens?

Accepted Answer

A) Differential interference 
B) Bright-field 
C) Electron 
D) Dark-field 
E) Phase-contrast 
A) Differential interference 
B) Bright-field 
C) Electron 
D) Dark-field 
E) Phase-contrast

Question 7

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 8

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A) Enriched 
B) Enumeration 
C) Selective 
D) Reducing 
E) Differential 
A) Enriched 
B) Enumeration 
C) Selective 
D) Reducing 
E) Differential

Question 9

Which of the following is not a benefit of agar as a solid medium?

Accepted Answer

A) Holds moisture 
B) Flexibility 
C) Solid at room temperature 
D) Can be inoculated and poured at a temperature that is not harmful 
E) Easily digested by most microbes 
A) Holds moisture 
B) Flexibility 
C) Solid at room temperature 
D) Can be inoculated and poured at a temperature that is not harmful 
E) Easily digested by most microbes

Question 10

Some microbes are not capable of growing on artificial media as pure cultures.

Accepted Answer

A) True 
 B)False

Question 11

Agar is an important component of media because

Accepted Answer

A) agar provides a solid surface for bacterial growth. 
B) bacteria require agar as a source of carbon. 
C) agar inhibits mold growth. 
D) agar prevents contamination. 
A) agar provides a solid surface for bacterial growth. 
B) bacteria require agar as a source of carbon. 
C) agar inhibits mold growth. 
D) agar prevents contamination.

Question 12

The correct microbiological term for the sample of specimen that is put into a nutrient medium to produce a culture is ______.

Accepted Answer

A) loop 
B) inoculum 
C) streak 
D) colony 
A) loop 
B) inoculum 
C) streak 
D) colony

Question 13

Which of the following parts of the microscope magnifies the specimen to produce the virtual image of the specimen?

Accepted Answer

A) Ocular lens 
B) Objective lens 
C) Condenser 
D) Body 
A) Ocular lens 
B) Objective lens 
C) Condenser 
D) Body

Question 14

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A) Transmission electron 
B) Differential interference contrast 
C) Scanning electron 
D) Phase-contrast 
E) Fluorescence 
A) Transmission electron 
B) Differential interference contrast 
C) Scanning electron 
D) Phase-contrast 
E) Fluorescence

Question 15

Which of the following parts of a microscope magnifies the specimen to produce a real image of the specimen?

Accepted Answer

A) Body 
B) Condenser 
C) Objective lens 
D) Ocular lens 
E) Nosepiece 
A) Body 
B) Condenser 
C) Objective lens 
D) Ocular lens 
E) Nosepiece

Question 16

A microbiologist makes a fixed smear of bacterial cells and stains it with methylene blue.All cells appear blue under the oil immersion lens.This is an example of ______.

Accepted Answer

A) simple staining 
B) negative staining 
C) capsule staining 
D) the acid-fast stain 
E) using an acidic dye 
A) simple staining 
B) negative staining 
C) capsule staining 
D) the acid-fast stain 
E) using an acidic dye

Question 17

A common medium for culturing fastidious bacteria is ______.

Accepted Answer

A) trypticase soy agar 
B) reducing medium 
C) mannitol salt agar 
D) blood agar 
E) MacConkey medium 
A) trypticase soy agar 
B) reducing medium 
C) mannitol salt agar 
D) blood agar 
E) MacConkey medium

Question 18

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 19

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Accepted Answer

A) Contrast 
B) Magnification 
C) Refraction 
D) Resolution 
A) Contrast 
B) Magnification 
C) Refraction 
D) Resolution

Question 20

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Accepted Answer

A) inoculation 
B) contamination 
C) infection 
D) immunization 
E) isolation 
A) inoculation 
B) contamination 
C) infection 
D) immunization 
E) isolation

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

What is the term for a culture made from one isolated colony?

What do the Gram stain, the acid-fast stain, and the endospore stain have in common?

Which type of medium would be the best choice when shipping a sample of bacteria from a clinic to a laboratory for testing?

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Which type of microscope achieves the greatest resolution and highest magnification?

Which type of microscope cannot image live specimens?

The bending of light rays as they pass from one medium to another is called refraction.

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Which of the following is not a benefit of agar as a solid medium?

Some microbes are not capable of growing on artificial media as pure cultures.

Agar is an important component of media because

The correct microbiological term for the sample of specimen that is put into a nutrient medium to produce a culture is ______.

Which of the following parts of the microscope magnifies the specimen to produce the virtual image of the specimen?

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Which of the following parts of a microscope magnifies the specimen to produce a real image of the specimen?

A microbiologist makes a fixed smear of bacterial cells and stains it with methylene blue.All cells appear blue under the oil immersion lens.This is an example of ______.

A common medium for culturing fastidious bacteria is ______.

The procedures for culturing a microorganism require the use of a microscope.

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

The Main Themes of Microbiology

The Chemistry of Biology

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Microbial Nutrition, Ecology, and Growth

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering and Recombinant Dna

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

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