Exam 10: Genetic Engineering and Recombinant Dna

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a specific technique called ______.

The field of pharmacogenomics uses knowledge of an individual's single nucleotide polymorphisms to determine how they will respond to a particular drug.

What type of DNA map is most detailed?

Analysis of DNA fragments in gel electrophoresis is based on

When patient tissues are transfected with viruses carrying a needed,normal human gene,the technique is called ______.

Amplification of DNA is accomplished by ______.

The primers in PCR are

The size of DNA is often given in the number of _____ that it contains.

Common vectors used to transfer a piece of DNA into a cloning host are

The advantage of germline therapy over somatic cell therapy is that the normal gene is inserted into an egg,sperm,or developing embryo so that the repair is present in every cell of the organism as it matures to adulthood.

Making new genomes is called ______.

Gene probes can be labeled for detection with reporter molecules such as _____.

The various techniques by which scientists manipulate DNA in the lab are termed ______.

DNA strands can begin to separate at the temperature of ______.

Which PCR step synthesizes complimentary DNA strands?

It is possible to identify mRNA molecules using fluorescently labeled cDNA.

Which of the following is the second step in gene mapping?

Why is an enzyme from a thermophilic bacterium used in PCR?

The difference between somatic cell gene therapy and germline therapy is that

Two different nucleic acids can _____ by annealing at their complementary sites.