Question 1

Geneticists can create sequences of DNA from RNA using enzymes called ______.

Accepted Answer

A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases 
A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases

Question 2

DNA fragments can be separated in gel electrophoresis because

Accepted Answer

A)  phosphate groups have a net negative charge. 
B)  nitrogenous bases have a net negative charge. 
C)  nitrogenous bases have a net positive charge. 
D)  phosphate groups have a net positive charge. 
A)  phosphate groups have a net negative charge. 
B)  nitrogenous bases have a net negative charge. 
C)  nitrogenous bases have a net positive charge. 
D)  phosphate groups have a net positive charge.

Question 3

The first genetically engineered protein approved for human use was ______.

Accepted Answer

A)  human insulin 
B)  hemophilia factor VIII 
C)  human adrenaline 
D)  human testosterone 
E)  human growth hormone 
A)  human insulin 
B)  hemophilia factor VIII 
C)  human adrenaline 
D)  human testosterone 
E)  human growth hormone

Question 4

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Accepted Answer

A)  PCR amplification to get more copies of DNA 
B)  Hybridization probes to digest the DNA sample 
C)  Electrophoresis to separate DNA fragments 
D)  Southern blot for a visual record of DNA fragments 
E)  Restriction endonucleases to cut DNA 
A)  PCR amplification to get more copies of DNA 
B)  Hybridization probes to digest the DNA sample 
C)  Electrophoresis to separate DNA fragments 
D)  Southern blot for a visual record of DNA fragments 
E)  Restriction endonucleases to cut DNA

Question 5

Transgenic animals are referred to as _____ modified organisms.

Accepted Answer

A)  chemically 
B)  physically 
C)  naturally 
D)  genetically 
A)  chemically 
B)  physically 
C)  naturally 
D)  genetically

Question 6

Which of the following is not true of fluorescent in situ hybridization (FISH)?

Accepted Answer

A)  Probes can be applied to intact cells. 
B)  Probes can locate genes on chromosomes. 
C)  It can be used to identify unknown bacteria without culturing. 
D)  It uses electrophoresis to separate the DNA. 
E)  It can be used to detect RNA in cells. 
A)  Probes can be applied to intact cells. 
B)  Probes can locate genes on chromosomes. 
C)  It can be used to identify unknown bacteria without culturing. 
D)  It uses electrophoresis to separate the DNA. 
E)  It can be used to detect RNA in cells.

Question 7

Transformation and transduction are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 8

Humans display how much DNA similarity with mice?

Accepted Answer

A)  50% 
B)  60% 
C)  70% 
D)  80% 
E)  90% 
A)  50% 
B)  60% 
C)  70% 
D)  80% 
E)  90%

Question 9

SNPs are derived from ______.

Accepted Answer

A)  genetic engineering 
B)  frameshift mutations 
C)  gene therapy 
D)  point mutations 
A)  genetic engineering 
B)  frameshift mutations 
C)  gene therapy 
D)  point mutations

Question 10

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Accepted Answer

A)  DNA fingerprinting 
B)  Antisense therapy 
C)  Gene therapy 
D)  Cloning 
A)  DNA fingerprinting 
B)  Antisense therapy 
C)  Gene therapy 
D)  Cloning

Question 11

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are ______.

Accepted Answer

A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases 
A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases

Question 12

Restriction endonucleases recognize and clip DNA base sequences called ______.

Accepted Answer

A)  codons 
B)  palindromes 
C)  introns 
D)  exons 
E)  genes 
A)  codons 
B)  palindromes 
C)  introns 
D)  exons 
E)  genes

Question 13

Microarray analysis is used to

Accepted Answer

A)  analyze an organism's entire genome. 
B)  determine which genes are being expressed in an organism. 
C)  determine the size of gene fragments after digesting with restriction endonucleases. 
D)  analyze an individual's single nucleotide polymorphisms. 
A)  analyze an organism's entire genome. 
B)  determine which genes are being expressed in an organism. 
C)  determine the size of gene fragments after digesting with restriction endonucleases. 
D)  analyze an individual's single nucleotide polymorphisms.

Question 14

E.coli is the best host for cloning because it possesses the mechanisms for processing and modifying proteins.

Accepted Answer

A) True 
 B)False

Question 15

Genetically modified organisms include _____.

Accepted Answer

A)  bacteria 
B)  viruses 
C)  plants 
D)  nonhuman animals 
E)  All of the above have been genetically modified. 
A)  bacteria 
B)  viruses 
C)  plants 
D)  nonhuman animals 
E)  All of the above have been genetically modified.

Question 16

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 17

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 18

Of the following choices,which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Accepted Answer

A)  Western Blot analysis 
B)  PCR 
C)  Microarray analysis 
D)  Transformation 
E)  Oryza sativa 
A)  Western Blot analysis 
B)  PCR 
C)  Microarray analysis 
D)  Transformation 
E)  Oryza sativa

Question 19

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Accepted Answer

A)  Pseudomonas syringae 
B)  Saccharomyces cerevisiae 
C)  Pseudomonas fluorescens 
D)  Escherichia coli 
E)  Thermus aquaticus 
A)  Pseudomonas syringae 
B)  Saccharomyces cerevisiae 
C)  Pseudomonas fluorescens 
D)  Escherichia coli 
E)  Thermus aquaticus

Question 20

Labeled,known,short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as ______.

Accepted Answer

A)  genetic engineering 
B)  biotechnology 
C)  recombinant DNA 
D)  gel electrophoresis 
E)  gene probes 
A)  genetic engineering 
B)  biotechnology 
C)  recombinant DNA 
D)  gel electrophoresis 
E)  gene probes

Exam 10: Genetic Engineering and Recombinant Dna

Geneticists can create sequences of DNA from RNA using enzymes called ______.

DNA fragments can be separated in gel electrophoresis because

The first genetically engineered protein approved for human use was ______.

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Transgenic animals are referred to as _____ modified organisms.

Which of the following is not true of fluorescent in situ hybridization (FISH)?

Transformation and transduction are methods used to introduce DNA into host cells.

Humans display how much DNA similarity with mice?

SNPs are derived from ______.

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are ______.

Restriction endonucleases recognize and clip DNA base sequences called ______.

Microarray analysis is used to

E.coli is the best host for cloning because it possesses the mechanisms for processing and modifying proteins.

Genetically modified organisms include _____.

Restriction endonucleases are obtained from various species of bacteria.

Reverse transcriptase is used to make cDNA from an RNA template.

Of the following choices,which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Labeled,known,short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as ______.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Host Defenses II: Specific Immunity and Immunization

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

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