Question 1

In choosing the vector used to carry human genes into a host cell,which of the following should be the important consideration?

Accepted Answer

A)  How much donor DNA can be carried on the vector 
B)  What kind of plasmid it is 
C)  Whether the vector comes from a bacterium or a virus 
D)  Whether the vector contains RNA or DNA 
A)  How much donor DNA can be carried on the vector 
B)  What kind of plasmid it is 
C)  Whether the vector comes from a bacterium or a virus 
D)  Whether the vector contains RNA or DNA

Question 2

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 3

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A) Add DNA polymerase and nucleotides at 72° C. 
B) Cool DNA to between 50° C and 65° C. 
C) Add primers. 
D) Heat target DNA to 94° C. 
E) Repeat the cycle of heating and cooling. 
A) Add DNA polymerase and nucleotides at 72° C. 
B) Cool DNA to between 50° C and 65° C. 
C) Add primers. 
D) Heat target DNA to 94° C. 
E) Repeat the cycle of heating and cooling.

Question 4

Which of the following recombinant DNA technology products is mismatched?

Accepted Answer

A)  Erythropoietin - stimulates erythrocyte production in bone marrow 
B)  Factor VIII - a protein necessary for the coagulation cascade 
C)  Interferons - stimulate antiviral protein production 
D)  Tissue plasminogen activating factor - stimulates blood clotting 
A)  Erythropoietin - stimulates erythrocyte production in bone marrow 
B)  Factor VIII - a protein necessary for the coagulation cascade 
C)  Interferons - stimulate antiviral protein production 
D)  Tissue plasminogen activating factor - stimulates blood clotting

Question 5

If you start with 3 double-stranded DNA fragments,after 4 cycles of PCR you will have ____ fragments.

Accepted Answer

A)  12 
B)  24 
C)  27 
D)  48 
E)  81 
A)  12 
B)  24 
C)  27 
D)  48 
E)  81

Question 6

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Accepted Answer

A)  DNA 
B)  libraries 
C)  clones 
D)  digests 
E)  books 
A)  DNA 
B)  libraries 
C)  clones 
D)  digests 
E)  books

Question 7

Transgenic animals

Accepted Answer

A)  can be engineered to become factories for manufacturing proteins. 
B)  are often obtained from germline engineering. 
C)  will pass the genes on to their offspring. 
D)  commonly include mice. 
E)  All of the choices are correct. 
A)  can be engineered to become factories for manufacturing proteins. 
B)  are often obtained from germline engineering. 
C)  will pass the genes on to their offspring. 
D)  commonly include mice. 
E)  All of the choices are correct.

Question 8

In the formation of recombinant DNA,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Accepted Answer

A)  DNA polymerase I 
B)  DNA polymerase II 
C)  DNA helicase 
D)  DNA ligase 
E)  Primase 
A)  DNA polymerase I 
B)  DNA polymerase II 
C)  DNA helicase 
D)  DNA ligase 
E)  Primase

Question 9

Micro RNA therapy is based on the premise that

Accepted Answer

A) 	miRNA molecules function to regulate gene expression, usually by inhibiting transcription; a non-functional miRNA can be replaced thereby reducing unwanted protein production. 
B) 	miRNA molecules function to regulate gene expression, usually by promoting translation; a non-functional miRNA can be repaired thereby reducing unwanted protein production. 
C) 	miRNA molecules function to regulate gene expression, usually by initiating transcription; an over-production of miRNA can be replaced thereby promoting necessary protein production. 
D) 	miRNA molecules function to regulate protein production, usually by inhibiting translation; a hyperactive miRNA can be replaced thereby reducing unwanted proteins. 
A) 	miRNA molecules function to regulate gene expression, usually by inhibiting transcription; a non-functional miRNA can be replaced thereby reducing unwanted protein production. 
B) 	miRNA molecules function to regulate gene expression, usually by promoting translation; a non-functional miRNA can be repaired thereby reducing unwanted protein production. 
C) 	miRNA molecules function to regulate gene expression, usually by initiating transcription; an over-production of miRNA can be replaced thereby promoting necessary protein production. 
D) 	miRNA molecules function to regulate protein production, usually by inhibiting translation; a hyperactive miRNA can be replaced thereby reducing unwanted proteins.

Question 10

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Accepted Answer

A) True 
 B)False

Question 11

Your infant has been diagnosed with severe combined immunodeficiency disease (SCID).Your doctor suggests which treatment as a possible cure for the disease?

Accepted Answer

A)  Genome mapping 
B)  Reverse transcription 
C)  Gene therapy 
D)  Microarrays 
A)  Genome mapping 
B)  Reverse transcription 
C)  Gene therapy 
D)  Microarrays

Question 12

When micro RNA molecules malfunction,their effect is limited to the inhibition of protein production,which results in tumor growth.

Accepted Answer

A) True 
 B)False

Question 13

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Accepted Answer

A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases 
A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases

Question 14

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 15

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Accepted Answer

A)  principal sources of restriction endonucleases. 
B)  used as cloning vectors. 
C)  genetically engineered bacteria. 
D)  sources of heat-stable DNA polymerases. 
A)  principal sources of restriction endonucleases. 
B)  used as cloning vectors. 
C)  genetically engineered bacteria. 
D)  sources of heat-stable DNA polymerases.

Question 16

When DNA is heated,the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 17

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA fingerprinting techniques.The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

Accepted Answer

A)  miRNAs 
B)  frameshift mutations 
C)  SNPs 
D)  microarrays 
A)  miRNAs 
B)  frameshift mutations 
C)  SNPs 
D)  microarrays

Question 18

A proteome is an organism's full array of expressed proteins,and proteomics is the study of those proteins and the functions they mediate.

Accepted Answer

A) True 
 B)False

Question 19

Which step in gene mapping can occur even more rapidly by PCR?

Accepted Answer

A)  Target DNA removed from cells and isolated 
B)  Cloning host treated with calcium chloride and receives plasmid 
C)  Target DNA and plasmid treated with the same restriction endonuclease 
D)  Desired protein is produced by cloning host 
E)  Gene is amplified by multiplication of cloning host 
A)  Target DNA removed from cells and isolated 
B)  Cloning host treated with calcium chloride and receives plasmid 
C)  Target DNA and plasmid treated with the same restriction endonuclease 
D)  Desired protein is produced by cloning host 
E)  Gene is amplified by multiplication of cloning host

Question 20

Transgenic organisms are ______.

Accepted Answer

A)  created in nature 
B)  only microorganisms 
C)  copyrighted 
D)  patented 
A)  created in nature 
B)  only microorganisms 
C)  copyrighted 
D)  patented

Exam 10: Genetic Engineering and Recombinant Dna

In choosing the vector used to carry human genes into a host cell,which of the following should be the important consideration?

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Which PCR step causes the denaturation of double-stranded DNA?

Which of the following recombinant DNA technology products is mismatched?

If you start with 3 double-stranded DNA fragments,after 4 cycles of PCR you will have ____ fragments.

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Transgenic animals

In the formation of recombinant DNA,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Micro RNA therapy is based on the premise that

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Your infant has been diagnosed with severe combined immunodeficiency disease (SCID).Your doctor suggests which treatment as a possible cure for the disease?

When micro RNA molecules malfunction,their effect is limited to the inhibition of protein production,which results in tumor growth.

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

When DNA is heated,the two strands will separate.

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA fingerprinting techniques.The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

A proteome is an organism's full array of expressed proteins,and proteomics is the study of those proteins and the functions they mediate.

Which step in gene mapping can occur even more rapidly by PCR?

Transgenic organisms are ______.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Host Defenses II: Specific Immunity and Immunization

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

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