Question 1

Outline the basic differences in function among RNA polymerases in both prokaryotes and eukaryotes.

Accepted Answer

Only one type of RNA polymerase is found in prokaryotes,so all classes of genes are transcribed by it.Eukaryotes have three distinct RNA polymerases,each of which transcribes different gene types.RNA polymerase I transcribes the genes for the 28S,18S,and 5.8S ribosomal RNAs; RNA polymerase II transcribes mRNA genes and some snRNA genes; and RNA polymerase III transcribes genes for the 5S rRNAs,the tRNAs,and the other snRNAs.

Question 2

A promoter is a coding portion of a gene.

Accepted Answer

A) True 
 B)False  False

Question 3

In eukaryotes,precursor mRNA molecules are processed in the

Accepted Answer

A) ribosomes. 
B) nucleus. 
C) mitochondria. 
D) cytoplasm. 
E) nucleolus. 
A) ribosomes. 
B) nucleus. 
C) mitochondria. 
D) cytoplasm. 
E) nucleolus. B

Question 4

Unlike eukaryotic mRNA,prokaryotic mRNA is polycistronic.

Accepted Answer

A) True 
 B)False

Question 5

In eukaryotes,the initiation complex binds DNA at the

Accepted Answer

A) Pribnow box. 
B) GC box. 
C) CAAT box. 
D) TATA box. 
E) GTF region. 
A) Pribnow box. 
B) GC box. 
C) CAAT box. 
D) TATA box. 
E) GTF region.

Question 6

Which of the following proteins is found only in the RNA polymerase holoenzyme?

Accepted Answer

A) Alpha 
B) Beta 
C) Epsilon 
D) Sigma 
E) Rho 
A) Alpha 
B) Beta 
C) Epsilon 
D) Sigma 
E) Rho

Question 7

Which type of RNA is found only in eukaryotes?

Accepted Answer

A) mRNA 
B) tRNA 
C) rRNA 
D) snRNA 
E) All of these 
A) mRNA 
B) tRNA 
C) rRNA 
D) snRNA 
E) All of these

Question 8

Which parts of a eukaryotic gene are transcribed?

Accepted Answer

A) Only the exons 
B) Only the introns 
C) Both the exons and introns 
D) Exons,introns,promoter,and terminator sequence 
E) It depends on the gene 
A) Only the exons 
B) Only the introns 
C) Both the exons and introns 
D) Exons,introns,promoter,and terminator sequence 
E) It depends on the gene

Question 9

Termination is carried out by RNA polymerase in a rho-independent terminator.

Accepted Answer

A) True 
 B)False

Question 10

Prokaryotic transcription begins when RNA polymerase binds to which site of the gene region?

Accepted Answer

A) -35 
B) -10 
C) +1 
D) +10 
E) +35 
A) -35 
B) -10 
C) +1 
D) +10 
E) +35

Question 11

The number of initiations of transcription from a promoter that has the sequence 5'-TAGCAT-3' will be greater than the number of initiations from a wild-type promoter.

Accepted Answer

A) True 
 B)False

Question 12

A prokaryotic mRNA transcript is

Accepted Answer

A) transcribed completely before being translated. 
B) further processed before it is transcribed. 
C) transported from the nucleus before it is translated. 
D) modified before it is translated. 
E) translated as it is being transcribed. 
A) transcribed completely before being translated. 
B) further processed before it is transcribed. 
C) transported from the nucleus before it is translated. 
D) modified before it is translated. 
E) translated as it is being transcribed.

Question 13

Posttranscriptional insertion or deletion of nucleotides that is absent in the DNA template is called

Accepted Answer

A) RNA splicing. 
B) RNA editing. 
C) RNA processing. 
D) RNA capping. 
E) RNA modification. 
A) RNA splicing. 
B) RNA editing. 
C) RNA processing. 
D) RNA capping. 
E) RNA modification.

Question 14

Group I introns,such as those found in Tetrahymena,are unique in that they are excised from mRNA by a protein-driven catalytic reaction.

Accepted Answer

A) True 
 B)False

Question 15

What kind of experiment or analysis could you conduct to define the location of the promoter elements of protein-coding genes?

Accepted Answer

You could examine the effect of mutation

Question 16

Cite at least three ways in which transcription differs from DNA replication.

Accepted Answer

(1)In replication,both strands of the do

Question 17

The single strand of mRNA that is produced during transcription has the same polarity as the ________ strand of DNA.

Accepted Answer

A) template 
B) nontemplate 
C) leading 
D) lagging 
E) nonsense 
A) template 
B) nontemplate 
C) leading 
D) lagging 
E) nonsense

Question 18

Activators are cis-acting sequences that increase transcription from a promoter.

Accepted Answer

A) True 
 B)False

Question 19

The sequence of a template strand of DNA is 3'-CATTACGCTT-5'.What is the sequence of the corresponding mRNA?

Accepted Answer

The answer of The sequence of a template strand of...

Question 20

RNA polymerase has no proofreading activity during transcription.

Accepted Answer

A) True 
 B)False

Exam 5: Gene Expression: Transcription

Outline the basic differences in function among RNA polymerases in both prokaryotes and eukaryotes.

A promoter is a coding portion of a gene.

In eukaryotes,precursor mRNA molecules are processed in the

Unlike eukaryotic mRNA,prokaryotic mRNA is polycistronic.

In eukaryotes,the initiation complex binds DNA at the

Which of the following proteins is found only in the RNA polymerase holoenzyme?

Which type of RNA is found only in eukaryotes?

Which parts of a eukaryotic gene are transcribed?

Termination is carried out by RNA polymerase in a rho-independent terminator.

Prokaryotic transcription begins when RNA polymerase binds to which site of the gene region?

The number of initiations of transcription from a promoter that has the sequence 5'-TAGCAT-3' will be greater than the number of initiations from a wild-type promoter.

A prokaryotic mRNA transcript is

Posttranscriptional insertion or deletion of nucleotides that is absent in the DNA template is called

Group I introns,such as those found in Tetrahymena,are unique in that they are excised from mRNA by a protein-driven catalytic reaction.

What kind of experiment or analysis could you conduct to define the location of the promoter elements of protein-coding genes?

Cite at least three ways in which transcription differs from DNA replication.

The single strand of mRNA that is produced during transcription has the same polarity as the ________ strand of DNA.

Activators are cis-acting sequences that increase transcription from a promoter.

The sequence of a template strand of DNA is 3'-CATTACGCTT-5'.What is the sequence of the corresponding mRNA?

RNA polymerase has no proofreading activity during transcription.

Genetics: An Introduction

DNA: The Genetic Material

DNA Replication

Gene Function

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Genomics: The Mapping and Sequencing of Genomes

Functional and Comparative Genomics

Recombinant DNA Technology

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

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