Question 1

The Ti plasmid is a natural plasmid in the soil bacterium Agrobacterium tumefaciens.

Accepted Answer

A) True 
 B)False  True

Question 2

Antisense mRNA can be used to regulate the expression of genes.Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-SavrTM tomato.

Accepted Answer

In engineered tomatoes,the gene for polygalacturonase,the enzyme responsible for fruit softening,was inserted backwards.This yielded an antisense mRNA copy that bonded with the sense mRNA,reducing the effective transcript level present for translation.Ripening was thus retarded relative to the normal tomato.

Question 3

A potentially serious problem with Taq polymerase in PCR is its lack of proofreading ability.Briefly explain why this can be a problem and at which stages during the PCR process this is most and least problematic.

Accepted Answer

Replication errors are introduced by Taq polymerase as a result of its lack of proofreading.The earlier they are introduced,the greater the number of descendant copies that will also have the error.Therefore,such errors are least problematic when they occur late in the PCR process.

Question 4

Humulin is insulin created by recombinant DNA technology.

Accepted Answer

A) True 
 B)False

Question 5

Two STR loci were determined to have the following allele frequencies in the general population:
$$\begin{array} { l l l } 
& \text { STR A } & \text { STR B } \
\text { Alleles: } & \text { 1A: } 0.1 & \text { 1B: } 0.4 \
& 2 \mathrm {~A} : 0.55 & 2 \mathrm {~B} : 0.4 \
& 3 \mathrm {~A} : 0.25 & \text { 3B: } 0.2 \
& 4 \mathrm {~A} : 0.1 &
\end{array}$$
A suspect in a criminal trial was genetically tested for these loci and was found to have the genotype (1A,1A)for STR A and (2B,2B)for STR B-homozygous for both loci.What is the probability that a randomly sampled person from this population would also have this genotype?

Accepted Answer

The product rule states that the net pro

Question 6

Genetic testing is typically done with blood samples,yet red blood cells (erythrocytes)are anucleate (lack nuclei)and are thus devoid of DNA.How,then,are these tests done?

Accepted Answer

Other blood cells,in particula

Question 7

Ti plasmids and the T-DNA system can be used to transform any vascular plant.

Accepted Answer

A) True 
 B)False

Question 8

How do genetic tests differ from diagnostic tests for genetic disease?

Accepted Answer

Genetic tests identify if an individual

Question 9

RFLPs arise due to

Accepted Answer

A) changes in the number of tandem repeats. 
B) base changes that add or delete restriction enzyme recognition sites. 
C) addition or deletion of bases between restriction enzyme recognition sites. 
D) B and C only 
E) All of these 
A) changes in the number of tandem repeats. 
B) base changes that add or delete restriction enzyme recognition sites. 
C) addition or deletion of bases between restriction enzyme recognition sites. 
D) B and C only 
E) All of these

Question 10

A couple with STR genotypes (50,75)and (50,100)(where the allele numbers denote repeat length)have several children.What are the possible genotypes of their children for this STR locus?

Accepted Answer

As for any single-locus trait,

Question 11

Rapid detection of SNPs,which are allelic variants,can be accomplished with DNA microarrays.If a microarray is set up for a set of genes,including all variants,outline a general procedure for quickly assaying the genotype of multiple individuals for that set of genes.

Accepted Answer

DNA taken from the individuals would be

Question 12

Briefly list the basic steps involved in constructing a DNA fingerprint for a paternity case.

Accepted Answer

Cut the DNA of each sample (child,mother

Question 13

Vectors capable of entering two or more different host organisms are known as

Accepted Answer

A) dual vectors. 
B) omnivectors. 
C) shuttle vectors. 
D) parity vectors. 
E) universal vectors. 
A) dual vectors. 
B) omnivectors. 
C) shuttle vectors. 
D) parity vectors. 
E) universal vectors.

Question 14

Match between columns
                        Premises: EPSPS
EST
SNP
ASO
STR

Accepted Answer

The Answer of Identified by mRNA and Stems from single is...

Question 15

A(n)________ is a photograph using X-ray film that is used to identify the position of radiolabeled molecules on a gel.

Accepted Answer

A) Southern blot 
B) northern blot 
C) X-rayogram 
D) autoradiogram 
E) radiocopy 
A) Southern blot 
B) northern blot 
C) X-rayogram 
D) autoradiogram 
E) radiocopy

Question 16

PCR has revolutionized the criminal justice system through DNA fingerprinting.For a given fragment of DNA,showing that a suspect's fingerprint differs from that found at a crime scene immediately excludes him or her from suspicion,yet finding a match does not mean that guilt can be automatically concluded.Why?

Accepted Answer

Different fragment lengths clearly must

Question 17

Which of the following is used to screen newborns for the genetic disease PKU?

Accepted Answer

A) RFLPs 
B) ASOs 
C) Antibodies 
D) PCR 
E) All of these 
A) RFLPs 
B) ASOs 
C) Antibodies 
D) PCR 
E) All of these

Question 18

DNase is used to treat hemophilia.

Accepted Answer

A) True 
 B)False

Question 19

Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector.These fusion proteins function by

Accepted Answer

A) binding to the lacZ operator,preventing transcription. 
B) binding to RNA polymerase,preventing transcription. 
C) binding to the lacZ repressor,enabling transcription. 
D) binding to the lacZ upstream activator sequence,enabling transcription. 
E) changing color in the presence of X-gal. 
A) binding to the lacZ operator,preventing transcription. 
B) binding to RNA polymerase,preventing transcription. 
C) binding to the lacZ repressor,enabling transcription. 
D) binding to the lacZ upstream activator sequence,enabling transcription. 
E) changing color in the presence of X-gal.

Question 20

Molecular DNA testing is used to test for aneuploids.

Accepted Answer

A) True 
 B)False

The Ti plasmid is a natural plasmid in the soil bacterium Agrobacterium tumefaciens.

Antisense mRNA can be used to regulate the expression of genes.Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-Savr^TM tomato.

A potentially serious problem with Taq polymerase in PCR is its lack of proofreading ability.Briefly explain why this can be a problem and at which stages during the PCR process this is most and least problematic.

Humulin is insulin created by recombinant DNA technology.

Genetic testing is typically done with blood samples,yet red blood cells (erythrocytes)are anucleate (lack nuclei)and are thus devoid of DNA.How,then,are these tests done?

Ti plasmids and the T-DNA system can be used to transform any vascular plant.

How do genetic tests differ from diagnostic tests for genetic disease?

RFLPs arise due to

A couple with STR genotypes (50,75)and (50,100)(where the allele numbers denote repeat length)have several children.What are the possible genotypes of their children for this STR locus?

Rapid detection of SNPs,which are allelic variants,can be accomplished with DNA microarrays.If a microarray is set up for a set of genes,including all variants,outline a general procedure for quickly assaying the genotype of multiple individuals for that set of genes.

Briefly list the basic steps involved in constructing a DNA fingerprint for a paternity case.

Vectors capable of entering two or more different host organisms are known as

A(n)________ is a photograph using X-ray film that is used to identify the position of radiolabeled molecules on a gel.

Which of the following is used to screen newborns for the genetic disease PKU?

DNase is used to treat hemophilia.

Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector.These fusion proteins function by

Molecular DNA testing is used to test for aneuploids.

Genetics: An Introduction

DNA: The Genetic Material

DNA Replication

Gene Function

Gene Expression: Transcription

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Genomics: The Mapping and Sequencing of Genomes

Functional and Comparative Genomics

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

Filters

Exam 10: Recombinant DNA Technology

The Ti plasmid is a natural plasmid in the soil bacterium Agrobacterium tumefaciens.

Antisense mRNA can be used to regulate the expression of genes.Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-SavrTM tomato.

A potentially serious problem with Taq polymerase in PCR is its lack of proofreading ability.Briefly explain why this can be a problem and at which stages during the PCR process this is most and least problematic.

Humulin is insulin created by recombinant DNA technology.

Genetic testing is typically done with blood samples,yet red blood cells (erythrocytes)are anucleate (lack nuclei)and are thus devoid of DNA.How,then,are these tests done?

Ti plasmids and the T-DNA system can be used to transform any vascular plant.

How do genetic tests differ from diagnostic tests for genetic disease?

RFLPs arise due to

A couple with STR genotypes (50,75)and (50,100)(where the allele numbers denote repeat length)have several children.What are the possible genotypes of their children for this STR locus?

Rapid detection of SNPs,which are allelic variants,can be accomplished with DNA microarrays.If a microarray is set up for a set of genes,including all variants,outline a general procedure for quickly assaying the genotype of multiple individuals for that set of genes.

Briefly list the basic steps involved in constructing a DNA fingerprint for a paternity case.

Vectors capable of entering two or more different host organisms are known as

A(n)________ is a photograph using X-ray film that is used to identify the position of radiolabeled molecules on a gel.

Which of the following is used to screen newborns for the genetic disease PKU?

DNase is used to treat hemophilia.

Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector.These fusion proteins function by

Molecular DNA testing is used to test for aneuploids.

Genetics: An Introduction

DNA: The Genetic Material

DNA Replication

Gene Function

Gene Expression: Transcription

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Genomics: The Mapping and Sequencing of Genomes

Functional and Comparative Genomics

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

Filters

Antisense mRNA can be used to regulate the expression of genes.Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-Savr^TM tomato.