Exam 8: Genomics: The Mapping and Sequencing of Genomes

Archaea are typically found in much the same habitats as bacteria.

DNA molecules,regardless of size,have a net positive charge.

The ________ method was used by the Celera Genomics Corporation to sequence the human genome.

The quality of an assembled sequence does not depend on the sequence coverage.

Successful insertion of a DNA fragment into the polylinker region of pBluescript II is detected by

How can the recircularization of a restriction-digested vector during a ligation reaction be minimized?

Which restriction enzyme leaves blunt ends?

The first complete nonviral genome sequenced was that of

A circular DNA molecule with two restriction enzyme cut sites will yield three fragments on digestion.

When DNA is cut with a restriction enzyme,the resulting fragments have

In DNA electrophoresis,fragment separation is based on

Please select the best match for each term. -Plasmid

Please select the best match for each term. -Dideoxynucleotides

Describe the basic approach to shotgun genome sequencing.

Please select the best match for each term. -YAC

A polylinker region in a cloning plasmid is characterized by

A plasmid used as a cloning vector in E.coli must have

Many bacteria are naturally capable of taking up endogenous fragments of DNA in their environment,a phenomenon termed

In gel electrophoresis,which of the following DNA fragments would travel the farthest distance from the sample well?