Question 1

Cloning vectors are used to introduce novel DNA into cells.

Accepted Answer

A) True 
 B)False

Question 2

The recognition sequences for many restriction enzymes are

Accepted Answer

A) terminal repeats. 
B) tandem repeats. 
C) palindromic. 
D) inverted repeats. 
E) dual repeats. 
A) terminal repeats. 
B) tandem repeats. 
C) palindromic. 
D) inverted repeats. 
E) dual repeats.

Question 3

Which types of cloning vector occur in nature?

Accepted Answer

A) Plasmids and cosmids 
B) Plasmids and bacteriophages 
C) Plasmids and BACs 
D) YACs and BACs 
E) Bacteriophages and cosmids 
A) Plasmids and cosmids 
B) Plasmids and bacteriophages 
C) Plasmids and BACs 
D) YACs and BACs 
E) Bacteriophages and cosmids

Question 4

In addition to restriction enzymes,which of the following enzymes is required to insert a fragment of DNA into a cloning vector?

Accepted Answer

A) DNA polymerase 
B) DNA ligase 
C) RNA polymerase 
D) Reverse transcriptase 
E) Topoisomerase 
A) DNA polymerase 
B) DNA ligase 
C) RNA polymerase 
D) Reverse transcriptase 
E) Topoisomerase

Question 5

Please select the best match for each term.
-ARS

Accepted Answer

A) Eukaryotic cloning vector 
B) Molecule used in DNA sequencing 
C) Origin of replication 
D) Small circular fragment of DNA 
E) Endonuclease isolated from bacteria 
A) Eukaryotic cloning vector 
B) Molecule used in DNA sequencing 
C) Origin of replication 
D) Small circular fragment of DNA 
E) Endonuclease isolated from bacteria

Question 6

Describe a DNA microarray setup for assaying mutations associated with a hypothetical genetic disease.

Accepted Answer

Normal and mutant gene sequences associa

Question 7

What advantage do cDNA libraries have over genomic libraries?

Accepted Answer

Eukaryotic genomes tend to have more non

Question 8

Sequencing the dog genome is currently a high priority for genomics researchers.What insights do you think can be gained about the evolutionary process by analyzing the genome of dogs?

Accepted Answer

Dogs are diversified into a multitude of

Question 9

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking ampS1U1P1RS1S1P0, however.
-Exactly why,in dideoxy DNA sequencing,do dideoxynucleotides halt the replication process?

Accepted Answer

Dideoxynucleotides are missing

Question 10

A restriction enzyme cuts DNA and leaves the following end: CTGCA G
Which of the following could be the sequence of the corresponding end of the other fragment generated by the enzyme?

Accepted Answer

A) G CTGCA 
B) C GTGCA 
C) CCGAT G 
D) GGCTA C 
E) None of these 
A) G CTGCA 
B) C GTGCA 
C) CCGAT G 
D) GGCTA C 
E) None of these

Question 11

Briefly contrast the dideoxy DNA sequencing developed by Fred Sanger with pyrosequencing methods.

Accepted Answer

Dideoxy DNA sequencing involves use of a

Question 12

What is a hapmap?

Accepted Answer

A hapmap is a complete descrip

Question 13

Based on DNA sequences of single genes,genomic analysis has confirmed that prokaryotes are divided into two great lineages: the Bacteria and the Archaea.The latter may be more closely related to the Eukarya.Recount the lines of evidence that support these findings.

Accepted Answer

Sequence divergence between archaeans an

Question 14

It is not always easy to locate or identify genes based on cDNA.How is this problem created by the differences between cDNA and DNA with respect to a given gene?

Accepted Answer

cDNA is made from mRNA,which i

Question 15

An SNP always occurs due to

Accepted Answer

A) a one base-pair change. 
B) a three base-pair change. 
C) a single restriction site change. 
D) a single amino acid change. 
E) None of these 
A) a one base-pair change. 
B) a three base-pair change. 
C) a single restriction site change. 
D) a single amino acid change. 
E) None of these

Question 16

A DNA copy of a messenger RNA molecule is called complementary DNA or cDNA.

Accepted Answer

A) True 
 B)False

Question 17

In partial restriction digestion,all available restriction sites will be cut.

Accepted Answer

A) True 
 B)False

Question 18

The chemical "label" that permits visualization of DNA fragments in an automated sequencing machine is

Accepted Answer

A) 32P. 
B) 35S. 
C) fluorescent dyes. 
D) tritium. 
E) None of these 
A) 32P. 
B) 35S. 
C) fluorescent dyes. 
D) tritium. 
E) None of these

Question 19

Cloning vectors generally possess unique restriction sites and dominant selectable markers.

Accepted Answer

A) True 
 B)False

Question 20

Please select the best match for each term.
-Restriction enzyme

Accepted Answer

A) Eukaryotic cloning vector 
B) Molecule used in DNA sequencing 
C) Origin of replication 
D) Small circular fragment of DNA 
E) Endonuclease isolated from bacteria 
A) Eukaryotic cloning vector 
B) Molecule used in DNA sequencing 
C) Origin of replication 
D) Small circular fragment of DNA 
E) Endonuclease isolated from bacteria

Exam 8: Genomics: The Mapping and Sequencing of Genomes

Cloning vectors are used to introduce novel DNA into cells.

The recognition sequences for many restriction enzymes are

Which types of cloning vector occur in nature?

In addition to restriction enzymes,which of the following enzymes is required to insert a fragment of DNA into a cloning vector?

Please select the best match for each term. -ARS

Describe a DNA microarray setup for assaying mutations associated with a hypothetical genetic disease.

What advantage do cDNA libraries have over genomic libraries?

Sequencing the dog genome is currently a high priority for genomics researchers.What insights do you think can be gained about the evolutionary process by analyzing the genome of dogs?

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking ampR, however. -Exactly why,in dideoxy DNA sequencing,do dideoxynucleotides halt the replication process?

A restriction enzyme cuts DNA and leaves the following end: CTGCA G Which of the following could be the sequence of the corresponding end of the other fragment generated by the enzyme?

Briefly contrast the dideoxy DNA sequencing developed by Fred Sanger with pyrosequencing methods.

What is a hapmap?

Based on DNA sequences of single genes,genomic analysis has confirmed that prokaryotes are divided into two great lineages: the Bacteria and the Archaea.The latter may be more closely related to the Eukarya.Recount the lines of evidence that support these findings.

It is not always easy to locate or identify genes based on cDNA.How is this problem created by the differences between cDNA and DNA with respect to a given gene?

An SNP always occurs due to

A DNA copy of a messenger RNA molecule is called complementary DNA or cDNA.

In partial restriction digestion,all available restriction sites will be cut.

The chemical "label" that permits visualization of DNA fragments in an automated sequencing machine is

Cloning vectors generally possess unique restriction sites and dominant selectable markers.

Please select the best match for each term. -Restriction enzyme

Genetics: An Introduction

DNA: The Genetic Material

DNA Replication

Gene Function

Gene Expression: Transcription

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Functional and Comparative Genomics

Recombinant DNA Technology

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

Filters

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking amp^R, however. -Exactly why,in dideoxy DNA sequencing,do dideoxynucleotides halt the replication process?