Question 1

Somatic cell gene therapy can result in a cure for a genetic disease in an individual as well as any progeny produced by the individual.

Accepted Answer

A) True 
 B)False

Question 2

How does a transcribable vector differ from an expression vector?

Accepted Answer

Transcribable vectors are desi

Question 3

DNA markers are DNA polymorphisms that are useful for genetic mapping.

Accepted Answer

A) True 
 B)False

Question 4

Match between columns
                        Premises: PCR
Northern blot
Electrophoresis
Southern blot
RFLPs

Accepted Answer

The Answer of Screening procedure used to assay DNA fragments is...

Question 5

________ blots are used to study DNA fragments,while ________ blots are used to study RNA fragments.

Accepted Answer

A) Western,northern 
B) Southern,western 
C) Eastern,northern 
D) Southern,northern 
E) Western,Southern 
A) Western,northern 
B) Southern,western 
C) Eastern,northern 
D) Southern,northern 
E) Western,Southern

Question 6

In yeast,expression of the GAL genes is induced when there is ________ in the culture medium.

Accepted Answer

A) glucose 
B) lactose 
C) galactose 
D) maltose 
E) Any of these 
A) glucose 
B) lactose 
C) galactose 
D) maltose 
E) Any of these

Question 7

DNA sequencing using the dideoxy sequencing method involves PCR reactions using Taq polymerase,which we learned in this chapter can introduce errors because it lacks a proofreading mechanism.What precaution(s)can be taken in genomic or other sequencing projects to minimize the possibility of producing DNA sequences with errors?

Accepted Answer

The most common precaution tak

Question 8

________ were the first class of DNA sequence polymorphisms discovered in the human genome.

Accepted Answer

A) SNPs 
B) Microsatellites 
C) VNTRs 
D) STRs 
E) RFLPs 
A) SNPs 
B) Microsatellites 
C) VNTRs 
D) STRs 
E) RFLPs

Question 9

Expressed sequence tags,or ESTs,

Accepted Answer

A) are genetic markers generated from cDNA. 
B) pertain to expressed genes only. 
C) are oligonucleotides derived from mRNA. 
D) A and C only 
E) All of these 
A) are genetic markers generated from cDNA. 
B) pertain to expressed genes only. 
C) are oligonucleotides derived from mRNA. 
D) A and C only 
E) All of these

Question 10

In a sequenced genome,candidate protein-encoding genes are identified by searching for

Accepted Answer

A) STRs. 
B) RFLPs. 
C) ESTs. 
D) SNPs. 
E) ORFs. 
A) STRs. 
B) RFLPs. 
C) ESTs. 
D) SNPs. 
E) ORFs.

Question 11

The Guthrie test is used to screen all newborns in the United States for cystic fibrosis.

Accepted Answer

A) True 
 B)False

Question 12

How does an RFLP help identify sickle-cell anemia?

Accepted Answer

The normal globin gene is associated wit

Question 13

When yeast cells are cultured in the presence of both glucose and galactose,the GAL genes are

Accepted Answer

A) transcribed and then translated. 
B) transcribed but not translated. 
C) neither transcribed nor translated. 
D) translated but not transcribed. 
E) transcribed and translated simultaneously. 
A) transcribed and then translated. 
B) transcribed but not translated. 
C) neither transcribed nor translated. 
D) translated but not transcribed. 
E) transcribed and translated simultaneously.

Question 14

DNA fingerprinting can be used to convict criminals,but has had little application in exonerating individuals already convicted.

Accepted Answer

A) True 
 B)False

Question 15

Study of P elements in Drosophila has shown that gene regulation can be accomplished by differential mRNA transcript processing.Explain.

Accepted Answer

The P element carries a single gene that

Question 16

Which of the following sequences is considered an STR?

Accepted Answer

A) CGTATTATGC 
B) TACGTACGTACG 
C) CAGCAGCAG 
D) TATTACGCCG 
E) A and B only 
A) CGTATTATGC 
B) TACGTACGTACG 
C) CAGCAGCAG 
D) TATTACGCCG 
E) A and B only

Question 17

RT-PCR is a PCR technique used in amplifying DNA copies of mRNA.

Accepted Answer

A) True 
 B)False

Question 18

Briefly explain how Ti plasmids induce crown gall (tumor)growth in plants parasitized by Agrobacterium tumefaciens,highlighting the specific properties that make this plasmid so useful in biotechnology.

Accepted Answer

Ti plasmids are so named because they ca

Question 19

Which of the following techniques is best suited to study protein interactions?

Accepted Answer

A) PCR 
B) The yeast two-hybrid system 
C) Restriction mapping 
D) RT-PCR 
E) Southern blotting 
A) PCR 
B) The yeast two-hybrid system 
C) Restriction mapping 
D) RT-PCR 
E) Southern blotting

Question 20

How has the Bt protein been used to increase insect-resistance in plants?

Accepted Answer

The Bt protein is a bacterial protein th

Exam 10: Recombinant DNA Technology

Somatic cell gene therapy can result in a cure for a genetic disease in an individual as well as any progeny produced by the individual.

How does a transcribable vector differ from an expression vector?

DNA markers are DNA polymorphisms that are useful for genetic mapping.

________ blots are used to study DNA fragments,while ________ blots are used to study RNA fragments.

In yeast,expression of the GAL genes is induced when there is ________ in the culture medium.

________ were the first class of DNA sequence polymorphisms discovered in the human genome.

Expressed sequence tags,or ESTs,

In a sequenced genome,candidate protein-encoding genes are identified by searching for

The Guthrie test is used to screen all newborns in the United States for cystic fibrosis.

How does an RFLP help identify sickle-cell anemia?

When yeast cells are cultured in the presence of both glucose and galactose,the GAL genes are

DNA fingerprinting can be used to convict criminals,but has had little application in exonerating individuals already convicted.

Study of P elements in Drosophila has shown that gene regulation can be accomplished by differential mRNA transcript processing.Explain.

Which of the following sequences is considered an STR?

RT-PCR is a PCR technique used in amplifying DNA copies of mRNA.

Briefly explain how Ti plasmids induce crown gall (tumor)growth in plants parasitized by Agrobacterium tumefaciens,highlighting the specific properties that make this plasmid so useful in biotechnology.

Which of the following techniques is best suited to study protein interactions?

How has the Bt protein been used to increase insect-resistance in plants?

Genetics: An Introduction

DNA: The Genetic Material

DNA Replication

Gene Function

Gene Expression: Transcription

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Genomics: The Mapping and Sequencing of Genomes

Functional and Comparative Genomics

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

Filters

blots are used to study DNA fragments,while blots are used to study RNA fragments.