Question 1

When combined with real-time PCR,melting curve analysis is done to:

Accepted Answer

A)  examine a single nucleotide variant genotype in a short amount of time. 
B)  quickly quantify the amplified target. 
C)  detect nucleic molecules at a specific wavelength. 
D)  examine the size of a fragment produced by RFLP. 
A)  examine a single nucleotide variant genotype in a short amount of time. 
B)  quickly quantify the amplified target. 
C)  detect nucleic molecules at a specific wavelength. 
D)  examine the size of a fragment produced by RFLP.

Question 2

When performing a PCR procedure,what is the best control to run to assess the presence of amplicon?

Accepted Answer

A)  Positive control 
B)  Blank control 
C)  Oligoligated control 
D)  dTTP control 
A)  Positive control 
B)  Blank control 
C)  Oligoligated control 
D)  dTTP control

Question 3

The annealing step in a PCR involves:

Accepted Answer

A)  binding of the primers to the single-stranded DNA. 
B)  unwinding and separating the double-stranded DNA. 
C)  adding nucleotides to primed sites of the DNA strands. 
D)  making a cDNA from an mRNA strand. 
A)  binding of the primers to the single-stranded DNA. 
B)  unwinding and separating the double-stranded DNA. 
C)  adding nucleotides to primed sites of the DNA strands. 
D)  making a cDNA from an mRNA strand.

Question 4

You have prepared a restriction enzyme digest of DNA from an individual who might have a genetic disorder.On the gel,you note that the digest from this individual produces one fragment,whereas the digest from a normal healthy individual produces two fragments.All controls worked correctly and you used the same reagents for all testing.What is your interpretation?

Accepted Answer

A)  The enzymes were not working. 
B)  The diseased individual's DNA is missing a restriction site. 
C)  You must have skipped the amplification step. 
D)  The normal individual's DNA is missing a restriction site. 
A)  The enzymes were not working. 
B)  The diseased individual's DNA is missing a restriction site. 
C)  You must have skipped the amplification step. 
D)  The normal individual's DNA is missing a restriction site.

Question 5

In a PCR,a control nucleic acid sequence that is different from the target sequence is added to each sample to:

Accepted Answer

A)  control for the presence of amplicon contamination. 
B)  bind aerosols. 
C)  control for the presence of inhibitors in the sample that might inhibit polymerase activity. 
D)  compare with an internal standard for quantitative analysis. 
A)  control for the presence of amplicon contamination. 
B)  bind aerosols. 
C)  control for the presence of inhibitors in the sample that might inhibit polymerase activity. 
D)  compare with an internal standard for quantitative analysis.

Exam 48: Nucleic Acid Techniques and Applications

When combined with real-time PCR,melting curve analysis is done to:

When performing a PCR procedure,what is the best control to run to assess the presence of amplicon?

The annealing step in a PCR involves:

In a PCR,a control nucleic acid sequence that is different from the target sequence is added to each sample to:

Clinical Chemistry, molecular Diagnostics, and Laboratory Medicine

Selection and Analytical Evaluation of Methodswith Statistical Techniques

Clinical Evaluation of Methods

Evidence-Based Laboratory Medicine

Establishment and Use of Reference Values

Specimen Collection, processing, and Other Preanalytical Variables

Quality Management

Principles of Basic Techniques and Laboratory Safety

Optical Techniques

Electrochemistry and Chemical Sensors

Electrophoresis

Chromatography

Mass Spectrometry

Enzyme and Rate Analyses

Immunochemical Techniques

Automation

Point-Of-Care Instrumentation

Amino Acids, peptides, and Proteins

Serum Enzymes

Tumor Markers and Cancer Genes

Kidney Function Test-Screatinine, Urea, and Uric Acid

Carbohydrates

Lipids, lip-Oproteins, apolipoproteins, and Other Cardiac Risk Factors

Electrolytes and Blood Gases

Hormones

Catecholamines and Serotonin

Vitamins, Trace Elements, and Nutritional Assessment

Hemoglobin, Iron, and Bilirubin

Porphyrins and Porphyrias

Therapeutic Drugs and Their Management

Clinical Toxicology

Toxic Metals

Diabetes

Cardiovascular Disease

Kidney Disease

Physiology and Disorders of Water,Electrolyte,and Acid-Base Metabolism

Liver Disease

Gastrointestinal and Pancreatic Diseases

Disorders of Bone and Mineral Metabolism

Disorders of the Pituitary

Disorders of the Adrenal Cortex

Thyroid Disorders

Reproduction-Related Disorders

Pregnancy and Prenatal Testing

Newborn Screening and Inborn Errors of Metabolism

Pharmacogenetics

Principles of Molecular Biology

Genomes and Nucleic Acid Alterations

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