Question 1

All of the following are examples of basic dyes except

Accepted Answer

A) Methylene Blue. 
B) Nigrosin. 
C) Crystal Violet. 
D) Safranin. 
E) Carbol Fuchsin. 
A) Methylene Blue. 
B) Nigrosin. 
C) Crystal Violet. 
D) Safranin. 
E) Carbol Fuchsin.

Question 2

Explain the difference and significance between a contaminated culture and a mixed culture.

Accepted Answer

A contaminated culture refers to a cultu

Question 3

A common medium used for growing fastidious bacteria is

Accepted Answer

A) blood agar. 
B) trypticase soy agar. 
C) mannitol salt agar. 
D) MacConkey medium. 
E) a reducing medium. 
A) blood agar. 
B) trypticase soy agar. 
C) mannitol salt agar. 
D) MacConkey medium. 
E) a reducing medium.

Question 4

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Accepted Answer

A) 100X 
B) 950X 
C) 85X 
D) 850X 
E) 95X 
A) 100X 
B) 950X 
C) 85X 
D) 850X 
E) 95X

Question 5

Bacteria that require special growth factors and complex organic substances are called

Accepted Answer

A) fastidious. 
B) pathogenic. 
C) harmless. 
D) anaerobic. 
E) aerobic. 
A) fastidious. 
B) pathogenic. 
C) harmless. 
D) anaerobic. 
E) aerobic.

Question 6

Which of the following magnifies the specimen to produce the real image of the specimen?

Accepted Answer

A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece 
A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece

Question 7

The primary purpose of staining cells on a microscope slide is to

Accepted Answer

A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility. 
A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility.

Question 8

Which microscope does not use light in forming the specimen image?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 9

Which microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 10

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Accepted Answer

A) True 
 B)False

Question 11

Which of the following media is useful for cultivating fungi?

Accepted Answer

A) Sabouraud's agar 
B) MacConkey agar 
C) Tomato juice agar 
D) Phenylethanol agar 
E) Mueller tellurite 
A) Sabouraud's agar 
B) MacConkey agar 
C) Tomato juice agar 
D) Phenylethanol agar 
E) Mueller tellurite

Question 12

A reducing medium contains

Accepted Answer

A) sugars that can be fermented. 
B) extra oxygen. 
C) hemoglobin, vitamins, or other growth factors. 
D) substances that remove oxygen. 
E) inhibiting agents. 
A) sugars that can be fermented. 
B) extra oxygen. 
C) hemoglobin, vitamins, or other growth factors. 
D) substances that remove oxygen. 
E) inhibiting agents.

Question 13

Which microscope is the most widely used to show stained cells against a bright background?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 14

Basic dyes are

Accepted Answer

A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin. 
A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin.

Question 15

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

The answer of Magnification is achieved in a compound microscope...

Question 16

Which of the following is essential for development of discrete, isolated colonies?

Accepted Answer

A) broth medium 
B) differential medium 
C) selective medium 
D) solid medium 
E) assay medium 
A) broth medium 
B) differential medium 
C) selective medium 
D) solid medium 
E) assay medium

Question 17

All of the following are correct about agar except

Accepted Answer

A) it is flexible. 
B) it melts at the boiling point of water (100$\circ$
C) . 
C) it is a source of nutrition for bacteria. 
D) it solidifies below 42$\circ$C. 
E) it is solid at room temperature. 
A) it is flexible. 
B) it melts at the boiling point of water (100$\circ$
C) . 
C) it is a source of nutrition for bacteria. 
D) it solidifies below 42$\circ$C. 
E) it is solid at room temperature.

Question 18

Which type of media can be used to determine if a bacteria is motile?

Accepted Answer

A) SIM 
B) MacConkey 
C) Enriched media 
D) Thayer-Martin media 
E) Chocolate agar 
A) SIM 
B) MacConkey 
C) Enriched media 
D) Thayer-Martin media 
E) Chocolate agar

Question 19

A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is:

Accepted Answer

A) selective only. 
B) differential only. 
C) both selective and differential. 
D) a reducing media. 
E) enriched. 
A) selective only. 
B) differential only. 
C) both selective and differential. 
D) a reducing media. 
E) enriched.

Question 20

Some microbes are not capable of growing on artificial media.

Accepted Answer

A) True 
 B)False

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

All of the following are examples of basic dyes except

Explain the difference and significance between a contaminated culture and a mixed culture.

A common medium used for growing fastidious bacteria is

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Bacteria that require special growth factors and complex organic substances are called

Which of the following magnifies the specimen to produce the real image of the specimen?

The primary purpose of staining cells on a microscope slide is to

Which microscope does not use light in forming the specimen image?

Which microscope achieves the greatest resolution and highest magnification?

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Which of the following media is useful for cultivating fungi?

A reducing medium contains

Which microscope is the most widely used to show stained cells against a bright background?

Basic dyes are

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Which of the following is essential for development of discrete, isolated colonies?

All of the following are correct about agar except

Which type of media can be used to determine if a bacteria is motile?

A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is:

Some microbes are not capable of growing on artificial media.

The Main Themes of Microbiology

The Chemistry of Biology

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

An Introduction to Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host-The Elements of Chemotherapy

Microbe-Human Interactions: Infection, Disease, and Epidemiology

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.