Question 1

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

The answer of The _____ of the microscope holds and...

Question 2

Brain-heart infusion, trypticase soy agar (TSA), and nutrient agar are all examples of which type of media?

Accepted Answer

A) synthetic 
B) reducing 
C) enriched 
D) nonsynthetic 
E) selective 
A) synthetic 
B) reducing 
C) enriched 
D) nonsynthetic 
E) selective

Question 3

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Accepted Answer

A) The microbiologist used too much inoculum. 
B) The culture is contaminated. 
C) The incubation temperature was incorrect. 
D) The culture medium must be selective. 
E) The culture medium must be differential. 
A) The microbiologist used too much inoculum. 
B) The culture is contaminated. 
C) The incubation temperature was incorrect. 
D) The culture medium must be selective. 
E) The culture medium must be differential.

Question 4

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Accepted Answer

Immersion oil increases resolution but n

Question 5

Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A) fluorescence 
B) differential interference contrast 
C) scanning electron 
D) transmission electron 
E) phase-contrast 
A) fluorescence 
B) differential interference contrast 
C) scanning electron 
D) transmission electron 
E) phase-contrast

Question 6

The three physical forms of laboratory media are: solid, semisolid, and _____.

Accepted Answer

The answer of The three physical forms of laboratory media...

Question 7

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

Accepted Answer

The answer of _____ dyes have a negative charge on...

Question 8

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 9

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Accepted Answer

A) negative staining. 
B) using an acidic dye. 
C) simple staining. 
D) using the acid-fast stain. 
E) capsule staining. 
A) negative staining. 
B) using an acidic dye. 
C) simple staining. 
D) using the acid-fast stain. 
E) capsule staining.

Question 10

A pure culture contains only

Accepted Answer

A) one species of microorganism. 
B) bacteria. 
C) a variety of microbes from one source. 
D) All of the choices are correct. 
E) None of the choices are correct. 
A) one species of microorganism. 
B) bacteria. 
C) a variety of microbes from one source. 
D) All of the choices are correct. 
E) None of the choices are correct.

Question 11

Which of the following reagents reacts with crystal violet as the mordant?

Accepted Answer

A) Crystal violet 
B) Gram's iodine 
C) 95% ethyl alcohol 
D) Safranin 
A) Crystal violet 
B) Gram's iodine 
C) 95% ethyl alcohol 
D) Safranin

Question 12

The wavelength of light used plus the numerical aperture governs

Accepted Answer

A) illumination. 
B) resolution. 
C) magnification. 
D) size of the field. 
E) All of the choices are correct. 
A) illumination. 
B) resolution. 
C) magnification. 
D) size of the field. 
E) All of the choices are correct.

Question 13

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing 
A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing

Question 14

All of the following are examples of different types of microbiological media except

Accepted Answer

A) broth. 
B) enriched. 
C) agar. 
D) petri dish. 
E) gelatin. 
A) broth. 
B) enriched. 
C) agar. 
D) petri dish. 
E) gelatin.

Question 15

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 16

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 17

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

Accepted Answer

A) True 
 B)False

Question 18

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A) hanging drop. 
B) fixed stained smear. 
C) Gram stain. 
D) negative stain. 
E) flagellar stain. 
A) hanging drop. 
B) fixed stained smear. 
C) Gram stain. 
D) negative stain. 
E) flagellar stain.

Question 19

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Accepted Answer

A) streak plate 
B) spread plate 
C) pour plate 
D) All of the choices are correct 
E) None of the choices are correct 
A) streak plate 
B) spread plate 
C) pour plate 
D) All of the choices are correct 
E) None of the choices are correct

Question 20

Scanning tunneling and atomic force microscopes are used to image the detailed structure of biological molecules.

Accepted Answer

A) True 
 B)False

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

The _____ of the microscope holds and allows selection of the objective lenses.

Brain-heart infusion, trypticase soy agar (TSA), and nutrient agar are all examples of which type of media?

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

The three physical forms of laboratory media are: solid, semisolid, and _____.

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

A medium that is gel-like has less agar in it compared to a solid medium.

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

A pure culture contains only

Which of the following reagents reacts with crystal violet as the mordant?

The wavelength of light used plus the numerical aperture governs

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

All of the following are examples of different types of microbiological media except

The bending of light rays as they pass from one medium to another is called refraction.

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

The specimen preparation that is best for viewing cell motility is

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Scanning tunneling and atomic force microscopes are used to image the detailed structure of biological molecules.

The Main Themes of Microbiology

The Chemistry of Biology

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

An Introduction to Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host-The Elements of Chemotherapy

Microbe-Human Interactions: Infection, Disease, and Epidemiology

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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