Question 1

Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A) fluorescence 
B) differential interference contrast 
C) scanning electron 
D) transmission electron 
E) phase-contrast 
A) fluorescence 
B) differential interference contrast 
C) scanning electron 
D) transmission electron 
E) phase-contrast

Question 2

Which of the following reagents reacts with crystal violet as the mordant?

Accepted Answer

A) Crystal violet 
B) Gram's iodine 
C) 95% ethyl alcohol 
D) Safranin 
A) Crystal violet 
B) Gram's iodine 
C) 95% ethyl alcohol 
D) Safranin

Question 3

The type of microscope in which you would see brightly illuminated specimens against a black background is

Accepted Answer

A) bright-field. 
B) dark-field. 
C) phase-contrast. 
D) fluorescence. 
E) electron. 
A) bright-field. 
B) dark-field. 
C) phase-contrast. 
D) fluorescence. 
E) electron.

Question 4

Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?

Accepted Answer

A) a pure culture 
B) a mixed culture 
C) a solid medium 
D) a liquid medium 
E) a contaminated medium 
A) a pure culture 
B) a mixed culture 
C) a solid medium 
D) a liquid medium 
E) a contaminated medium

Question 5

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible would be termed

Accepted Answer

A) complex. 
B) reducing. 
C) enriched. 
D) enumeration. 
E) synthetic. 
A) complex. 
B) reducing. 
C) enriched. 
D) enumeration. 
E) synthetic.

Question 6

The primary purpose of staining cells on a microscope slide is to

Accepted Answer

A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility. 
A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility.

Question 7

A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is

Accepted Answer

A) selective only. 
B) differential only. 
C) both selective and differential. 
D) a reducing media. 
E) enriched. 
A) selective only. 
B) differential only. 
C) both selective and differential. 
D) a reducing media. 
E) enriched.

Question 8

Bacteria that require special growth factors and complex organic substances are called

Accepted Answer

A) fastidious. 
B) pathogenic. 
C) harmless. 
D) anaerobic. 
E) aerobic. 
A) fastidious. 
B) pathogenic. 
C) harmless. 
D) anaerobic. 
E) aerobic.

Question 9

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

Accepted Answer

The answer of _____ dyes have a negative charge on...

Question 10

A contaminated food sample contains several different species of bacteria.A food microbiologist is interested in studying just one of these species.Describe the sequence of procedures that the microbiologist must perform in order to obtain a pure culture of the bacterial species of interest from this food sample.Detail all the necessary media and equipment.

Accepted Answer

The microbiologist must first obtain a s

Question 11

How will

Accepted Answer

A) Gram-positive 
B) Gram-negative 
C) Gram-variable 
D) None of these 
E) coli appear if the mordant is not applied? 
A) Gram-positive 
B) Gram-negative 
C) Gram-variable 
D) None of these 
E) coli appear if the mordant is not applied?

Question 12

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except

Accepted Answer

A) 0.2 µm. 
B) 0.2 mm. 
C) 0.1 µm. 
D) 0.3 µm. 
E) 2.0 µm. 
A) 0.2 µm. 
B) 0.2 mm. 
C) 0.1 µm. 
D) 0.3 µm. 
E) 2.0 µm.

Question 13

Explain the difference and significance between a contaminated culture and a mixed culture.

Accepted Answer

A contaminated culture refers to a cultu

Question 14

The procedure for culturing a microorganism requires the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 15

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

The answer of The _____ of the microscope holds and...

Question 16

Basic dyes are

Accepted Answer

A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin. 
A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin.

Question 17

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A) hanging drop. 
B) fixed stained smear. 
C) gram stain. 
D) negative stain. 
E) flagellar stain. 
A) hanging drop. 
B) fixed stained smear. 
C) gram stain. 
D) negative stain. 
E) flagellar stain.

Question 18

Brain-heart infusion, trypticase soy agar (TSA), and nutrient agar are all examples of which type of media?

Accepted Answer

A) synthetic 
B) reducing 
C) enriched 
D) nonsynthetic 
E) selective 
A) synthetic 
B) reducing 
C) enriched 
D) nonsynthetic 
E) selective

Question 19

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.Following incubation, only the E.coli grows in the culture.What is the most likely explanation?

Accepted Answer

A) The microbiologist used too much inoculum. 
B) The culture is contaminated. 
C) The incubation temperature was incorrect. 
D) The culture medium must be selective. 
E) The culture medium must be differential. 
A) The microbiologist used too much inoculum. 
B) The culture is contaminated. 
C) The incubation temperature was incorrect. 
D) The culture medium must be selective. 
E) The culture medium must be differential.

Question 20

All of the following are examples of different types of microbiological media except

Accepted Answer

A) broth. 
B) enriched. 
C) agar. 
D) petri dish. 
E) gelatin. 
A) broth. 
B) enriched. 
C) agar. 
D) petri dish. 
E) gelatin.

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Which of the following reagents reacts with crystal violet as the mordant?

The type of microscope in which you would see brightly illuminated specimens against a black background is

Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible would be termed

The primary purpose of staining cells on a microscope slide is to

A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is

Bacteria that require special growth factors and complex organic substances are called

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

How will

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except

Explain the difference and significance between a contaminated culture and a mixed culture.

The procedure for culturing a microorganism requires the use of a microscope.

The _____ of the microscope holds and allows selection of the objective lenses.

Basic dyes are

The specimen preparation that is best for viewing cell motility is

Brain-heart infusion, trypticase soy agar (TSA), and nutrient agar are all examples of which type of media?

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.Following incubation, only the E.coli grows in the culture.What is the most likely explanation?

All of the following are examples of different types of microbiological media except

The Main Themes of Microbiology

The Chemistry of Biology

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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