Question 1

This microscope achieves the greatest resolution and highest magnification:

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 2

The Gram stain, acid-fast stain, and endospore stain have the following in common:

Accepted Answer

A) used on a wet mount of the specimen 
B) use heat to force the dye into cell structures 
C) outcome based on cell wall differences 
D) use a negative stain technique 
E) are differential stains 
A) used on a wet mount of the specimen 
B) use heat to force the dye into cell structures 
C) outcome based on cell wall differences 
D) use a negative stain technique 
E) are differential stains

Question 3

Which of the following puts the Six "I"s in their correct order?

Accepted Answer

A) inoculation, incubation, isolation, inspection, identification 
B) isolation, inspection, inoculation, incubation, identification 
C) incubation, inspection, isolation, identification, inoculation 
D) inspection, identification, isolation, incubation, inoculation 
E) inspection, isolation, incubation, inoculation, identification 
A) inoculation, incubation, isolation, inspection, identification 
B) isolation, inspection, inoculation, incubation, identification 
C) incubation, inspection, isolation, identification, inoculation 
D) inspection, identification, isolation, incubation, inoculation 
E) inspection, isolation, incubation, inoculation, identification

Question 4

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Accepted Answer

A) Sterile swab, loop dilution, TSB 
B) Hockey stick, spread plate technique, and 4 degree Celsius incubation temperature 
C) Loop dilution, TSA, hockey stick, 37 degree Celsius incubator 
D) Inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator 
E) Sterile swab, streak plate method, 37 degree Celsius incubator 
A) Sterile swab, loop dilution, TSB 
B) Hockey stick, spread plate technique, and 4 degree Celsius incubation temperature 
C) Loop dilution, TSA, hockey stick, 37 degree Celsius incubator 
D) Inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator 
E) Sterile swab, streak plate method, 37 degree Celsius incubator

Question 5

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Accepted Answer

Immersion oil increases resolution but n

Question 6

Which type of media can be used to determine if a bacteria is motile?

Accepted Answer

A) SIM 
B) MacConkey 
C) Enriched media 
D) Thayer-Martin media 
E) Chocolate agar 
A) SIM 
B) MacConkey 
C) Enriched media 
D) Thayer-Martin media 
E) Chocolate agar

Question 7

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

Accepted Answer

The answer of The correct microbiological term for the tiny...

Question 8

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing 
A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing

Question 9

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Accepted Answer

A) NaCl 
B) sheep red blood cells 
C) bile salts 
D) thioglycolic acid 
E) peptone 
A) NaCl 
B) sheep red blood cells 
C) bile salts 
D) thioglycolic acid 
E) peptone

Question 10

All of the following are correct about agar except

Accepted Answer

A) it is flexible. 
B) it melts at the boiling point of water (100°
C) . 
C) it is a source of nutrition for bacteria. 
D) it solidifies below 42°C. 
E) it is solid at room temperature. 
A) it is flexible. 
B) it melts at the boiling point of water (100°
C) . 
C) it is a source of nutrition for bacteria. 
D) it solidifies below 42°C. 
E) it is solid at room temperature.

Question 11

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following magnifies the specimen to produce the real image of the specimen?

Accepted Answer

A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece 
A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece

Question 13

A microbiologist decides to use a nutrient medium that contains thioglycolic acid.What type of microbe is she attempting to culture?

Accepted Answer

A) fastidious 
B) gram positive 
C) anaerobe 
D) gram negative 
E) virus 
A) fastidious 
B) gram positive 
C) anaerobe 
D) gram negative 
E) virus

Question 14

This microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities:

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 15

Compare and contrast the reagents and functions of negative staining versus positive staining.

Accepted Answer

Negative staining and positive staining

Question 16

All of the following are examples of basic dyes except

Accepted Answer

A) methylene blue. 
B) nigrosin. 
C) crystal violet. 
D) safranin. 
E) fuchsin. 
A) methylene blue. 
B) nigrosin. 
C) crystal violet. 
D) safranin. 
E) fuchsin.

Question 17

The three physical forms of laboratory media are: solid, semisolid, and _____.

Accepted Answer

The answer of The three physical forms of laboratory media...

Question 18

The wavelength of light used plus the numerical aperture governs

Accepted Answer

A) illumination. 
B) resolution. 
C) magnification. 
D) size of the field. 
E) All of the choices are correct. 
A) illumination. 
B) resolution. 
C) magnification. 
D) size of the field. 
E) All of the choices are correct.

Question 19

This microscope does not use light in forming the specimen image:

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 20

A pure culture contains only

Accepted Answer

A) one species of microorganism. 
B) bacteria. 
C) a variety of microbes from one source. 
D) None of the choices are correct. 
A) one species of microorganism. 
B) bacteria. 
C) a variety of microbes from one source. 
D) None of the choices are correct.

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

This microscope achieves the greatest resolution and highest magnification:

The Gram stain, acid-fast stain, and endospore stain have the following in common:

Which of the following puts the Six "I"s in their correct order?

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Which type of media can be used to determine if a bacteria is motile?

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

All of the following are correct about agar except

The bending of light rays as they pass from one medium to another is called refraction.

Which of the following magnifies the specimen to produce the real image of the specimen?

A microbiologist decides to use a nutrient medium that contains thioglycolic acid.What type of microbe is she attempting to culture?

This microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities:

Compare and contrast the reagents and functions of negative staining versus positive staining.

All of the following are examples of basic dyes except

The three physical forms of laboratory media are: solid, semisolid, and _____.

The wavelength of light used plus the numerical aperture governs

This microscope does not use light in forming the specimen image:

A pure culture contains only

The Main Themes of Microbiology

The Chemistry of Biology

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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