Question 1

In addition to yeast cells,Gal4 has been shown to activate transcription in:

Accepted Answer

A) insect cells. 
B) mouse cells. 
C) human cells. 
D) insect,mouse,and human cells. 
E) None of the answer options are correct. 
A) insect cells. 
B) mouse cells. 
C) human cells. 
D) insect,mouse,and human cells. 
E) None of the answer options are correct. D

Question 2

The miRNA-RISC complex is known to inhibit translation.List several possible mechanisms for translational inhibition mediated by miRNA-RISC.

Accepted Answer

RISC interferes with cap binding (initiation),prevents ribosome assembly (initiation),interferes with elongation,and removes the poly(A)tail,thereby hastening mRNA degradation.

Question 3

In yeast,which of these is NOT a function of MAT genes?

Accepted Answer

A) controlling the expression of structural genes in a and α cells 
B) MATa locus encoding a regulatory protein (a1)that affects only diploid cells 
C) MATa locus encoding a regulatory protein (a1)that affects both haploid and diploid cells. 
D) MATα allele encoding a regulatory protein (α2)that represses expression of a specific genes 
E) None of the answer options are correct. 
A) controlling the expression of structural genes in a and α cells 
B) MATa locus encoding a regulatory protein (a1)that affects only diploid cells 
C) MATa locus encoding a regulatory protein (a1)that affects both haploid and diploid cells. 
D) MATα allele encoding a regulatory protein (α2)that represses expression of a specific genes 
E) None of the answer options are correct. C

Question 4

In eukaryotes,primary mRNA transcripts are extensively processed,which include(s):

Accepted Answer

A) modification of the 5′ end. 
B) modification of the 3′ end. 
C) removal of introns by splicing. 
D) None of the answer options are correct. 
E) modification of either end and removal of introns by splicing. 
A) modification of the 5′ end. 
B) modification of the 3′ end. 
C) removal of introns by splicing. 
D) None of the answer options are correct. 
E) modification of either end and removal of introns by splicing.

Question 5

The GAL4 enhancers are located _____ of the genes they regulate and are known as ______.

Accepted Answer

A) downstream;downstream activation sequences (DAS) 
B) downstream;downstream enhancer sequences (DES) 
C) upstream;upstream activation sequences (UAS) 
D) upstream;upstream enhancer elements (UES) 
E) None of the answer options are correct. 
A) downstream;downstream activation sequences (DAS) 
B) downstream;downstream enhancer sequences (DES) 
C) upstream;upstream activation sequences (UAS) 
D) upstream;upstream enhancer elements (UES) 
E) None of the answer options are correct.

Question 6

Which of the following GAL genes are located on the same chromosome?

Accepted Answer

A) GAL1 and GAL2 
B) GAL1 and GAL10 
C) GAL2 and GAL10 
D) GAL2 and GAL7 
E) All of the answer options are correct. 
A) GAL1 and GAL2 
B) GAL1 and GAL10 
C) GAL2 and GAL10 
D) GAL2 and GAL7 
E) All of the answer options are correct.

Question 7

Genomic imprinting in mice is mediated by the imprinting control region (ICR).In the paternal allele the:

Accepted Answer

A) enhancer element is methylated and serves as a buffer-insulator. 
B) ICR is methylated,which allows H19 transcription. 
C) ICR is not methylated,which allows H19 transcription. 
D) ICR is not methylated,which allows Igf2 transcription. 
E) ICR is methylated,which allows Igf2 transcription. 
A) enhancer element is methylated and serves as a buffer-insulator. 
B) ICR is methylated,which allows H19 transcription. 
C) ICR is not methylated,which allows H19 transcription. 
D) ICR is not methylated,which allows Igf2 transcription. 
E) ICR is methylated,which allows Igf2 transcription.

Question 8

Which of the following describes the histones associated with the nucleosomes of inactive genes?

Accepted Answer

A) poor in acetyl groups (i.e. ,hypoacetylated) 
B) rich in acetyl groups (i.e. ,hyperacetylated) 
C) rich in phosphate groups (i.e. ,hyperphosphorylated) 
D) poor in phosphate groups (i.e. ,hypophosphorylated) 
E) None of the answer options are correct. 
A) poor in acetyl groups (i.e. ,hypoacetylated) 
B) rich in acetyl groups (i.e. ,hyperacetylated) 
C) rich in phosphate groups (i.e. ,hyperphosphorylated) 
D) poor in phosphate groups (i.e. ,hypophosphorylated) 
E) None of the answer options are correct.

Question 9

GAL80 inhibits GAL gene expression by:

Accepted Answer

A) specifically binding to Gal4 and preventing DNA-binding. 
B) specifically binding to Gal4 and preventing activation. 
C) specifically binding to Gal3 and preventing DNA-binding. 
D) specifically binding to Gal3 and preventing activation. 
E) inducing an allosteric change in Gal3. 
A) specifically binding to Gal4 and preventing DNA-binding. 
B) specifically binding to Gal4 and preventing activation. 
C) specifically binding to Gal3 and preventing DNA-binding. 
D) specifically binding to Gal3 and preventing activation. 
E) inducing an allosteric change in Gal3.

Question 10

Compared to heterochromatin,euchromatin is:

Accepted Answer

A) rich in genes and comprised of densely packed nucleosomes. 
B) poor in genes and comprised of densely packed nucleosomes. 
C) rich in genes and comprised of loosely packed nucleosomes. 
D) poor in genes and comprised of loosely packed nucleosomes. 
E) rich in genes and completely lacking nucleosomes. 
A) rich in genes and comprised of densely packed nucleosomes. 
B) poor in genes and comprised of densely packed nucleosomes. 
C) rich in genes and comprised of loosely packed nucleosomes. 
D) poor in genes and comprised of loosely packed nucleosomes. 
E) rich in genes and completely lacking nucleosomes.

Question 11

DNA elements that prevent the spreading of heterochromatin into adjacent euchromatic regions are called:

Accepted Answer

A) enhancer blocking elements. 
B) euchromatin buffers. 
C) barrier insulators. 
D) barrier buffers. 
E) barrier-blocking elements. 
A) enhancer blocking elements. 
B) euchromatin buffers. 
C) barrier insulators. 
D) barrier buffers. 
E) barrier-blocking elements.

Question 12

Genomic imprinting in mice is mediated by the imprinting control region (ICR).In the maternal allele the:

Accepted Answer

A) enhancer element is methylated and serves as a buffer-insulator. 
B) ICR is methylated,which allows H19 transcription. 
C) ICR is not methylated,which allows H19 transcription. 
D) ICR is not methylated,which allows Igf2 transcription. 
E) ICR is methylated,which allows Igf2 transcription. 
A) enhancer element is methylated and serves as a buffer-insulator. 
B) ICR is methylated,which allows H19 transcription. 
C) ICR is not methylated,which allows H19 transcription. 
D) ICR is not methylated,which allows Igf2 transcription. 
E) ICR is methylated,which allows Igf2 transcription.

Question 13

Methylation of H3 lysine residue 4:

Accepted Answer

A) is associated with activation of gene expression. 
B) is associated with repression of gene expression. 
C) has no effect on gene expression. 
D) creates binding sites for other gene repressors. 
E) None of the answer options are correct. 
A) is associated with activation of gene expression. 
B) is associated with repression of gene expression. 
C) has no effect on gene expression. 
D) creates binding sites for other gene repressors. 
E) None of the answer options are correct.

Question 14

The GAL4 protein has which of the following functional domains?

Accepted Answer

A) a DNA-binding domain 
B) an activation domain 
C) a repressor domain 
D) a DNA-binding domain and an activation domain 
E) a DNA-binding domain and a repressor domain 
A) a DNA-binding domain 
B) an activation domain 
C) a repressor domain 
D) a DNA-binding domain and an activation domain 
E) a DNA-binding domain and a repressor domain

Question 15

The ________________ is a cis-acting element that binds trans-acting DNA-binding proteins.

Accepted Answer

A) promoter 
B) enhancer 
C) promoter-proximal element 
D) GC-rich box 
E) All of the answer options are correct. 
A) promoter 
B) enhancer 
C) promoter-proximal element 
D) GC-rich box 
E) All of the answer options are correct.

Question 16

Which histone is considered the "linker" that compacts nucleosomes into higher order structures?

Accepted Answer

A) H1 
B) H2A 
C) H2B 
D) H3 
E) H4 
A) H1 
B) H2A 
C) H2B 
D) H3 
E) H4

Question 17

Expression of a gene in a mammal can sometimes be associated with the parent from which the gene was inherited (mother or father).What term is used to describe the situation where an allele that is inherited from the father is expressed,but the homologous allele inherited from the mother is not?

Accepted Answer

A) paternal imprinting 
B) maternal imprinting 
C) autosomal imprinting 
D) Y-linked inheritance 
E) X-linked inheritance 
A) paternal imprinting 
B) maternal imprinting 
C) autosomal imprinting 
D) Y-linked inheritance 
E) X-linked inheritance

Question 18

The enzyme responsible for removing acetyl groups from histone proteins is called:

Accepted Answer

A) histone acetylase (H
A) . 
B) histone deacetylase (HDA
C) . 
C) histone acetylmethyltransferase (HAT). 
D) histone acetyltransferase (HAT). 
E) histone methyltransferase (HMT). 
A) histone acetylase (H
A) . 
B) histone deacetylase (HDA
C) . 
C) histone acetylmethyltransferase (HAT). 
D) histone acetyltransferase (HAT). 
E) histone methyltransferase (HMT).

Question 19

The mating-type (MAT)locus in yeast has how many alleles?

Accepted Answer

A) 1 
B) 2 
C) 3 
D) 4 
E) The precise number is unknown. 
A) 1 
B) 2 
C) 3 
D) 4 
E) The precise number is unknown.

Question 20

The ________________ is a cis-acting element that binds regulatory proteins.

Accepted Answer

A) promoter 
B) operator 
C) promoter-proximal element 
D) TATA box 
E) All of the answer options are correct. 
A) promoter 
B) operator 
C) promoter-proximal element 
D) TATA box 
E) All of the answer options are correct.

In addition to yeast cells,Gal4 has been shown to activate transcription in:

The miRNA-RISC complex is known to inhibit translation.List several possible mechanisms for translational inhibition mediated by miRNA-RISC.

In yeast,which of these is NOT a function of MAT genes?

In eukaryotes,primary mRNA transcripts are extensively processed,which include(s):

The GAL4 enhancers are located _ of the genes they regulate and are known as __.

Which of the following GAL genes are located on the same chromosome?

Genomic imprinting in mice is mediated by the imprinting control region (ICR).In the paternal allele the:

Which of the following describes the histones associated with the nucleosomes of inactive genes?

GAL80 inhibits GAL gene expression by:

Compared to heterochromatin,euchromatin is:

DNA elements that prevent the spreading of heterochromatin into adjacent euchromatic regions are called:

Genomic imprinting in mice is mediated by the imprinting control region (ICR).In the maternal allele the:

Methylation of H3 lysine residue 4:

The GAL4 protein has which of the following functional domains?

The ________________ is a cis-acting element that binds trans-acting DNA-binding proteins.

Which histone is considered the "linker" that compacts nucleosomes into higher order structures?

Expression of a gene in a mammal can sometimes be associated with the parent from which the gene was inherited (mother or father).What term is used to describe the situation where an allele that is inherited from the father is expressed,but the homologous allele inherited from the mother is not?

The enzyme responsible for removing acetyl groups from histone proteins is called:

The mating-type (MAT)locus in yeast has how many alleles?

The ________________ is a cis-acting element that binds regulatory proteins.

The Genetics Revolution

Single-Gene Inheritance

Independent Assortment of Genes

Mapping Eukaryote Chromosomes by Recombination

The Genetics of Bacteria and Their Viruses

Gene Interaction

Dna: Structure and Replication

Rna: Transcription and Processing

Proteins and Their Synthesis

Gene Isolation and Manipulation

Regulation of Gene Expression in Bacteria and Their Viruses

The Genetic Control of Development

Genomes and Genomics

The Dynamic Genome: Transposable Elements

Mutation, Repair, and Recombination

Large-Scale Chromosomal Changes

Population Genetics

The Inheritance of Complex Traits

Evolution of Genes and Traits

Filters

Exam 12: Regulation of Gene Expression in Eukaryotes

In addition to yeast cells,Gal4 has been shown to activate transcription in:

The miRNA-RISC complex is known to inhibit translation.List several possible mechanisms for translational inhibition mediated by miRNA-RISC.

In yeast,which of these is NOT a function of MAT genes?

In eukaryotes,primary mRNA transcripts are extensively processed,which include(s):

The GAL4 enhancers are located _____ of the genes they regulate and are known as ______.

Which of the following GAL genes are located on the same chromosome?

Genomic imprinting in mice is mediated by the imprinting control region (ICR).In the paternal allele the:

Which of the following describes the histones associated with the nucleosomes of inactive genes?

GAL80 inhibits GAL gene expression by:

Compared to heterochromatin,euchromatin is:

DNA elements that prevent the spreading of heterochromatin into adjacent euchromatic regions are called:

Genomic imprinting in mice is mediated by the imprinting control region (ICR).In the maternal allele the:

Methylation of H3 lysine residue 4:

The GAL4 protein has which of the following functional domains?

The ________________ is a cis-acting element that binds trans-acting DNA-binding proteins.

Which histone is considered the "linker" that compacts nucleosomes into higher order structures?

Expression of a gene in a mammal can sometimes be associated with the parent from which the gene was inherited (mother or father).What term is used to describe the situation where an allele that is inherited from the father is expressed,but the homologous allele inherited from the mother is not?

The enzyme responsible for removing acetyl groups from histone proteins is called:

The mating-type (MAT)locus in yeast has how many alleles?

The ________________ is a cis-acting element that binds regulatory proteins.

The Genetics Revolution

Single-Gene Inheritance

Independent Assortment of Genes

Mapping Eukaryote Chromosomes by Recombination

The Genetics of Bacteria and Their Viruses

Gene Interaction

Dna: Structure and Replication

Rna: Transcription and Processing

Proteins and Their Synthesis

Gene Isolation and Manipulation

Regulation of Gene Expression in Bacteria and Their Viruses

The Genetic Control of Development

Genomes and Genomics

The Dynamic Genome: Transposable Elements

Mutation, Repair, and Recombination

Large-Scale Chromosomal Changes

Population Genetics

The Inheritance of Complex Traits

Evolution of Genes and Traits

Filters

The GAL4 enhancers are located _ of the genes they regulate and are known as __.