Exam 8: Rna: Transcription and Processing

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Name the three major modifications of mRNA in eukaryotes before it is transported to the cytoplasm.In addition,tell why each of the modifications is necessary.

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1)Add 5′ cap to protect against RNA degradation and to act as a binding site for the initiation of translation;
2)add poly-A tail to the 3′ end of the transcript to protect against degradation and aid in the transfer of the mRNA to the cytoplasm;and
3)remove introns to generate an intact open reading frame for translation.

The carboxyl tail domain of RNA polymerase II plays a key role in:

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E

Why is transcription in eukaryotes more complex than in prokaryotes?

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D

The "RNA World" theory suggests that:

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Which of the following mRNA codons would form a codon-anticodon base pairing interaction with the 3′-UAG-5′ tRNA anticodon?

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RNA synthesis is always 5' to 3' because:

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If the DNA template 5′-ATGCATGC-3′ were transcribed to RNA,the RNA would read:

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A key characteristic of bacterial RNAs that is not observed with eukaryotic RNAs is that:

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The initial discovery of the spliceosome resulted from experiments focused on:

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RNA polymerase can transcribe in different directions on a chromosome.This is enabled because:

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The role of tRNA is to:

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These functional RNAs function to regulate protein production of specific genes.

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For protein-encoding genes,six general transcription factors (GTFs)function to:

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Which of the following is evidence that RNA was a message-carrying intermediary between DNA and protein?

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Before the completion of the human genome project,it was generally estimated that human DNA would contain up to 100,000 genes.We now know humans contain only around 25,000 individual genes within our genomes.This "gene count" is so surprising because:

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The spliceosome functions to:

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The experimental value of a "pulse-chase" cellular labeling experiment using radioactive uracil is that:

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The -10 and the -35 boxes found in bacterial gene promoters function to:

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Which of the following acts before the others?

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What are the three major classes of functional RNAs? Briefly describe the role of each class of functional RNA.

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