Exam 5: Methods in Protein Biochemistry

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions.Which fraction corresponds to the cytosol fraction?

In tandem mass spectrometry,the first mass spectrometer

Compare the order of migration and the separation of three proteins (25 kDa,100 kDa,and 150 kDa)in an SDS-PAGE gel with 15% polyacrylamide gel.

In isoelectric focusing,

Contrast the way that SDS-PAGE and native PAGE gels separate proteins.

Monoclonal antibodies

During solid-phase peptide synthesis,dicyclohexylcarbodiimide is used

Which technique ionizes polypeptides by embedding the tryptic fragments into a light-absorbing matrix and exposing it to a laser?

During the production of polyclonal antibodies,where are the antibodies isolated from?

Which method uses mass to charge ratios as well as a bioinformatics approach to determine the amino acid composition of a protein?

Explain the difference between polyclonal and monoclonal antibodies.

Describe how polyclonal antibodies are generated.

The step in solid-phase peptide synthesis that occurs at number 3 is

Why is the supply of polyclonal antibodies limited?

The advantage of using a native PAGE gel compared with an SDS-PAGE gel is that the native PAGE gel

In tandem mass spectrometry,the second mass spectrometer

Predict the fragments of the following peptide after cleavage by trypsin. GLMKTYPDSTA

Describe how an ELISA detects antigenic proteins.

After centrifugation,the purity of the protein is determined by

The size of proteins studied by NMR is limited because