Question 1

Which technique can be combined with mass spectrometry to identify protein antigens in large cellular complexes?

Accepted Answer

A)  immunofluorescence 
B)  Western blot 
C)  immunoprecipitation 
D)  ELISA 
A)  immunofluorescence 
B)  Western blot 
C)  immunoprecipitation 
D)  ELISA

Question 2

Biochemical assays are important in the process of isolating proteins because they

Accepted Answer

A)  uniquely identify one protein. 
B)  separate proteins based on polarity. 
C)  isolate proteins based on size. 
D)  determine the molecular weight of proteins. 
A)  uniquely identify one protein. 
B)  separate proteins based on polarity. 
C)  isolate proteins based on size. 
D)  determine the molecular weight of proteins.

Question 3

When cells isolated from the spleen are fused with long-living cells,they produce

Accepted Answer

A)  B cells. 
B)  immortalized tumor cells. 
C)  hybridoma cells. 
D)  red blood cells. 
A)  B cells. 
B)  immortalized tumor cells. 
C)  hybridoma cells. 
D)  red blood cells.

Question 4

Theoretically,an individual B cell could express any combination of light chain and heavy chain polypeptides to produce one of 10 antibody complexes.How does this seemingly limited production of antibodies protect us from the tens of millions of foreign antigens we encounter through our lives?

Accepted Answer

DNA recombination is the reason that we

Question 5

Using the figure below describing monoclonal antibody generation,identify the step where the B cells are fused with the tumor cells.

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4

Question 6

What cells are shown at step number 5 in the figure below?

Accepted Answer

A)  B cells 
B)  immortalized tumor cell 
C)  hybridoma cells 
D)  red blood cells 
A)  B cells 
B)  immortalized tumor cell 
C)  hybridoma cells 
D)  red blood cells

Question 7

What cells are shown at step number 2 in the figure below?

Accepted Answer

A)  B cells 
B)  immortalized tumor cells 
C)  hybridoma cells 
D)  red blood cells 
A)  B cells 
B)  immortalized tumor cells 
C)  hybridoma cells 
D)  red blood cells

Question 8

What is the advantage of using monoclonal antibodies compared with polyclonal antibodies?

Accepted Answer

A)  They are less labor intensive to generate. 
B)  They can recognize more than one epitope. 
C)  The process used to produce the antibodies provides unlimited supplies. 
D)  They can be generated without using animals. 
A)  They are less labor intensive to generate. 
B)  They can recognize more than one epitope. 
C)  The process used to produce the antibodies provides unlimited supplies. 
D)  They can be generated without using animals.

Question 9

Compare and contrast the three MOST commonly used homogenization techniques to prepare a cell extract.

Accepted Answer

All three methods are used to disrupt or

Question 10

Which enzyme or reagent cleaves a peptide at the carboxyl side of a methionine residue?

Accepted Answer

A)  trypsin 
B)  chymotrypsin 
C)  V-8 protease 
D)  cyanogen bromide 
A)  trypsin 
B)  chymotrypsin 
C)  V-8 protease 
D)  cyanogen bromide

Question 11

What is the advantage of using polyclonal antibodies compared with monoclonal antibodies?

Accepted Answer

A)  They are less labor intensive to generate. 
B)  They provide the ability to monitor quality continually. 
C)  The process used to produce the antibodies provides unlimited supplies. 
D)  They can be generated without using animals. 
A)  They are less labor intensive to generate. 
B)  They provide the ability to monitor quality continually. 
C)  The process used to produce the antibodies provides unlimited supplies. 
D)  They can be generated without using animals.

Question 12

Which percentage of polyacrylamide would give the best separation for small proteins?

Accepted Answer

A)  5% 
B)  7.5% 
C)  10% 
D)  20% 
A)  5% 
B)  7.5% 
C)  10% 
D)  20%

Question 13

Explain how proteins are separated using isoelectric focusing.

Accepted Answer

Proteins are applied to a gel strip cont

Question 14

Which technique ionizes polypeptides by releasing them from a small metallic capillary at high voltage?

Accepted Answer

A)  ESI 
B)  MALDI 
C)  NMR 
D)  X-ray crystallography 
A)  ESI 
B)  MALDI 
C)  NMR 
D)  X-ray crystallography

Question 15

You are employed in a prominent biochemistry lab,ProteinsRUs.The company offers a $500 bonus to the person who can develop a way to perform Western blotting without having to wait to make a unique antibody for proteins under analysis.How would you propose to do this? Explain the method you would use in two to three sentences and give an example.

Accepted Answer

A faster way to perform the analysis wou

Question 16

The advantage of using an SDS-PAGE gel compared with a native PAGE gel is that the SDS-PAGE gel

Accepted Answer

A)  separates proteins only based on molar mass. 
B)  gives information about the charge or conformation of the protein. 
C)  results in a better separation of small proteins. 
D)  increases the resolution of large and small proteins. 
A)  separates proteins only based on molar mass. 
B)  gives information about the charge or conformation of the protein. 
C)  results in a better separation of small proteins. 
D)  increases the resolution of large and small proteins.

Question 17

In monoclonal antibody generation,the cells that produce the antibody in the animal are located in __________ cells.

Accepted Answer

A)  heart 
B)  liver 
C)  spleen 
D)  red blood 
A)  heart 
B)  liver 
C)  spleen 
D)  red blood

Question 18

Which separation technique exploits the solubility differences of proteins?

Accepted Answer

A)  centrifugation 
B)  salting out 
C)  dialysis 
D)  ion-exchange chromatography 
A)  centrifugation 
B)  salting out 
C)  dialysis 
D)  ion-exchange chromatography

Question 19

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions.Which fraction corresponds to the mitochondrial fraction?

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
A)  A 
B)  B 
C)  C 
D)  D

Question 20

Two-dimensional polyacrylamide gel electrophoresis separates proteins based on

Accepted Answer

A)  pI and shape. 
B)  ligand affinity and molecular weight. 
C)  shape and ligand affinity. 
D)  pI and molecular weight. 
A)  pI and shape. 
B)  ligand affinity and molecular weight. 
C)  shape and ligand affinity. 
D)  pI and molecular weight.

Exam 5: Methods in Protein Biochemistry

Which technique can be combined with mass spectrometry to identify protein antigens in large cellular complexes?

Biochemical assays are important in the process of isolating proteins because they

When cells isolated from the spleen are fused with long-living cells,they produce

Theoretically,an individual B cell could express any combination of light chain and heavy chain polypeptides to produce one of 10 antibody complexes.How does this seemingly limited production of antibodies protect us from the tens of millions of foreign antigens we encounter through our lives?

Using the figure below describing monoclonal antibody generation,identify the step where the B cells are fused with the tumor cells.

What cells are shown at step number 5 in the figure below?

What cells are shown at step number 2 in the figure below?

What is the advantage of using monoclonal antibodies compared with polyclonal antibodies?

Compare and contrast the three MOST commonly used homogenization techniques to prepare a cell extract.

Which enzyme or reagent cleaves a peptide at the carboxyl side of a methionine residue?

What is the advantage of using polyclonal antibodies compared with monoclonal antibodies?

Which percentage of polyacrylamide would give the best separation for small proteins?

Explain how proteins are separated using isoelectric focusing.

Which technique ionizes polypeptides by releasing them from a small metallic capillary at high voltage?

The advantage of using an SDS-PAGE gel compared with a native PAGE gel is that the SDS-PAGE gel

In monoclonal antibody generation,the cells that produce the antibody in the animal are located in __________ cells.

Which separation technique exploits the solubility differences of proteins?

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions.Which fraction corresponds to the mitochondrial fraction?

Two-dimensional polyacrylamide gel electrophoresis separates proteins based on

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters