Question 1

Which of the following best describes homogeneous assays?

Accepted Answer

A)  A washing step is necessary. 
B)  Concentration of patient antigen is directly proportional to the label detected. 
C)  The reagent antibody has an enzyme tag. 
D)  Labeled and unlabeled analyte are present in equal amounts. 
A)  A washing step is necessary. 
B)  Concentration of patient antigen is directly proportional to the label detected. 
C)  The reagent antibody has an enzyme tag. 
D)  Labeled and unlabeled analyte are present in equal amounts. B

Question 2

Which of the following best describes competitive binding assays?

Accepted Answer

A)  A limited number of binding sites are present. 
B)  Concentration of patient antigen is directly proportional to the label detected. 
C)  All patient antigen present is allowed to bind. 
D)  Labeled and unlabeled analyte are present in equal amounts. 
A)  A limited number of binding sites are present. 
B)  Concentration of patient antigen is directly proportional to the label detected. 
C)  All patient antigen present is allowed to bind. 
D)  Labeled and unlabeled analyte are present in equal amounts. A

Question 3

An immunoassay was performed in the following way: Antigen is bound to a solid support.Patient serum is added and patient antibodies bind to the antigen.The well is washed and enzyme-labeled anti-immunoglobulin antibodies are added.The well is washed and enzyme substrate is added and enzyme activity is determined.Which immunoassay is described?

Accepted Answer

A)  sandwich 
B)  competitive 
C)  ELISA 
D)  homogeneous 
A)  sandwich 
B)  competitive 
C)  ELISA 
D)  homogeneous C

Question 4

Pregnancy testing is based on an early increase in which hormone?

Accepted Answer

A)  human chorionic gonadotropin 
B)  luteinizing hormone 
C)  thyroid-stimulating hormone 
D)  follicle-stimulating hormone 
A)  human chorionic gonadotropin 
B)  luteinizing hormone 
C)  thyroid-stimulating hormone 
D)  follicle-stimulating hormone

Question 5

All of the following are characteristic of noncompetitive enzyme immunoassays EXCEPT:

Accepted Answer

A)  They are more sensitive than agglutination reactions. 
B)  Concentration is directly proportional to the amount of color. 
C)  Washing carefully is very important. 
D)  Binding sites for patient antibody are limited. 
A)  They are more sensitive than agglutination reactions. 
B)  Concentration is directly proportional to the amount of color. 
C)  Washing carefully is very important. 
D)  Binding sites for patient antibody are limited.

Question 6

In the following equation,10 Ag* + 5 Ag + 3 Ab ---> Ag*Ab + Ag Ab + Ag* + Ag,the ratio of bound radioactive antigen to bound patient antigen is:

Accepted Answer

A)  2:1 
B)  1:2 
C)  3:1 
D)  5:1 
A)  2:1 
B)  1:2 
C)  3:1 
D)  5:1

Question 7

In the following equation,what is the ratio of bound radioactive antigen to bound patient antigen? 8ag* + 4ag + 6ab ---> ag*ab + agab + ag* + ag

Accepted Answer

A)  1:6 
B)  1:3 
C)  2:1 
D)  8:6 
A)  1:6 
B)  1:3 
C)  2:1 
D)  8:6

Question 8

In an indirect fluorescent immunoassay such as the FTA confirmatory test for syphilis,all of the following are true EXCEPT:

Accepted Answer

A)  A labeled antigen is used. 
B)  Washing is an important step. 
C)  Patient antibody is detected. 
D)  Antihuman globulin has a fluorescent tag. 
A)  A labeled antigen is used. 
B)  Washing is an important step. 
C)  Patient antibody is detected. 
D)  Antihuman globulin has a fluorescent tag.

Question 9

Indirect immunofluorescence is similar to which type of enzyme immunoassay as far as the performance of the assay?

Accepted Answer

A)  competitive 
B)  sandwich 
C)  ELISA 
D)  homogeneous 
A)  competitive 
B)  sandwich 
C)  ELISA 
D)  homogeneous

Question 10

An immunoassay is performed in the following manner to look for an Ag in the patient serum: Patient serum + labeled Ag + known Ab are added together and incubated.Unbound material is washed off.The amount of labeled Ag is then measured.What assay is performed?

Accepted Answer

A)  homogeneous EIA 
B)  competitive EIA 
C)  sandwich EIA 
D)  ELISA 
A)  homogeneous EIA 
B)  competitive EIA 
C)  sandwich EIA 
D)  ELISA

Question 11

In which of the following testing situations is there a direct relationship between the amount of bound label and the amount of patient antigen present?

Accepted Answer

A)  competitive RIA using labeled antigen 
B)  competitive EIA using labeled antigen 
C)  noncompetitive EIA using a second labeled antibody 
D)  any of the above 
A)  competitive RIA using labeled antigen 
B)  competitive EIA using labeled antigen 
C)  noncompetitive EIA using a second labeled antibody 
D)  any of the above

Question 12

Which of the following is characteristic of noncompetitive enzyme immunoassays?

Accepted Answer

A)  Binding sites for patient antigen are limited. 
B)  Washing in between steps is not necessary. 
C)  All patient antigen is allowed to react with binding sites. 
D)  Color is indirectly proportional to the concentration. 
A)  Binding sites for patient antigen are limited. 
B)  Washing in between steps is not necessary. 
C)  All patient antigen is allowed to react with binding sites. 
D)  Color is indirectly proportional to the concentration.

Question 13

In a sandwich or capture assay to determine the presence of a viral antigen in a patient sample,which of the following would be on solid phase?

Accepted Answer

A)  antigen 
B)  labeled antibody 
C)  unlabeled antibody 
D)  antihuman immunoglobulin 
A)  antigen 
B)  labeled antibody 
C)  unlabeled antibody 
D)  antihuman immunoglobulin

Question 14

Components of a competitive immunoassay include all of the following EXCEPT:

Accepted Answer

A)  labeled analyte 
B)  means of detection of the label 
C)  solid-phase antibody 
D)  standard different from the analyte 
A)  labeled analyte 
B)  means of detection of the label 
C)  solid-phase antibody 
D)  standard different from the analyte

Question 15

An ELISA assay was performed on a patient's serum sample.What is the relationship of the amount of enzyme activity to the amount of patient analyte measured?

Accepted Answer

A)  directly proportional 
B)  indirectly proportional 
A)  directly proportional 
B)  indirectly proportional

Which of the following best describes homogeneous assays?

Which of the following best describes competitive binding assays?

Pregnancy testing is based on an early increase in which hormone?

All of the following are characteristic of noncompetitive enzyme immunoassays EXCEPT:

In the following equation,10 Ag* + 5 Ag + 3 Ab ---> AgAb + Ag Ab + Ag + Ag,the ratio of bound radioactive antigen to bound patient antigen is:

In the following equation,what is the ratio of bound radioactive antigen to bound patient antigen? 8ag* + 4ag + 6ab ---> agab + agab + ag + ag

In an indirect fluorescent immunoassay such as the FTA confirmatory test for syphilis,all of the following are true EXCEPT:

Indirect immunofluorescence is similar to which type of enzyme immunoassay as far as the performance of the assay?

An immunoassay is performed in the following manner to look for an Ag in the patient serum: Patient serum + labeled Ag + known Ab are added together and incubated.Unbound material is washed off.The amount of labeled Ag is then measured.What assay is performed?

In which of the following testing situations is there a direct relationship between the amount of bound label and the amount of patient antigen present?

Which of the following is characteristic of noncompetitive enzyme immunoassays?

In a sandwich or capture assay to determine the presence of a viral antigen in a patient sample,which of the following would be on solid phase?

Components of a competitive immunoassay include all of the following EXCEPT:

An ELISA assay was performed on a patient's serum sample.What is the relationship of the amount of enzyme activity to the amount of patient analyte measured?

Introduction and Natural Immunity

Lymphoid System

Nature of Antigens and Major Histocompatibility Complex

Antibody Structure and Function

Cytokines

Complement System

Safety and Specimen Preparation

Precipitation Reactions

Agglutination

Molecular Techniques

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Immunoproliferative Diseases

Immunodeficiency Disease

Transplantation Immunology

Tumor Immunology

Serologic and Molecular Detection of Bacterial Infections

Serologic and Molecular Detection of Bacterial Infections

Serology of Parasitic and Fungal Infections

Spirochete Diseases

Filters

Exam 10: Labeled Immunoassays

Which of the following best describes homogeneous assays?

Which of the following best describes competitive binding assays?

Pregnancy testing is based on an early increase in which hormone?

All of the following are characteristic of noncompetitive enzyme immunoassays EXCEPT:

In the following equation,10 Ag* + 5 Ag + 3 Ab ---> Ag*Ab + Ag Ab + Ag* + Ag,the ratio of bound radioactive antigen to bound patient antigen is:

In the following equation,what is the ratio of bound radioactive antigen to bound patient antigen? 8ag* + 4ag + 6ab ---> ag*ab + agab + ag* + ag

In an indirect fluorescent immunoassay such as the FTA confirmatory test for syphilis,all of the following are true EXCEPT:

Indirect immunofluorescence is similar to which type of enzyme immunoassay as far as the performance of the assay?

An immunoassay is performed in the following manner to look for an Ag in the patient serum: Patient serum + labeled Ag + known Ab are added together and incubated.Unbound material is washed off.The amount of labeled Ag is then measured.What assay is performed?

In which of the following testing situations is there a direct relationship between the amount of bound label and the amount of patient antigen present?

Which of the following is characteristic of noncompetitive enzyme immunoassays?

In a sandwich or capture assay to determine the presence of a viral antigen in a patient sample,which of the following would be on solid phase?

Components of a competitive immunoassay include all of the following EXCEPT:

An ELISA assay was performed on a patient's serum sample.What is the relationship of the amount of enzyme activity to the amount of patient analyte measured?

Introduction and Natural Immunity

Lymphoid System

Nature of Antigens and Major Histocompatibility Complex

Antibody Structure and Function

Cytokines

Complement System

Safety and Specimen Preparation

Precipitation Reactions

Agglutination

Molecular Techniques

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Immunoproliferative Diseases

Immunodeficiency Disease

Transplantation Immunology

Tumor Immunology

Serologic and Molecular Detection of Bacterial Infections

Serologic and Molecular Detection of Bacterial Infections

Serology of Parasitic and Fungal Infections

Spirochete Diseases

Filters

In the following equation,10 Ag* + 5 Ag + 3 Ab ---> AgAb + Ag Ab + Ag + Ag,the ratio of bound radioactive antigen to bound patient antigen is:

In the following equation,what is the ratio of bound radioactive antigen to bound patient antigen? 8ag* + 4ag + 6ab ---> agab + agab + ag + ag