Question 1

Mitochondrial polymorphisms used for human identification are found in what part of the mitochondrial genome?

Accepted Answer

A)  In the mitochondrial tRNA genes 
B)  At the origin of transcription 
C)  In the hypervariable regions 
D)  Anywhere on the circular genome 
A)  In the mitochondrial tRNA genes 
B)  At the origin of transcription 
C)  In the hypervariable regions 
D)  Anywhere on the circular genome C

Question 2

Two peaks from different sources falling within a bin describe what relationship?

Accepted Answer

A)  Different alleles 
B)  Identical alleles 
C)  Paternity 
D)  Exclusion 
A)  Different alleles 
B)  Identical alleles 
C)  Paternity 
D)  Exclusion B

Question 3

Which of the following is used as a marker of gender?

Accepted Answer

A)  D8S1179 
B)  Amelogenin 
C)  TPOX 
D)  Penta D 
A)  D8S1179 
B)  Amelogenin 
C)  TPOX 
D)  Penta D B

Question 4

How are mitochondrial polymorphisms inherited?

Accepted Answer

A)  Paternally 
B)  Maternally 
C)  In multiples of six bases 
D)  Randomly 
A)  Paternally 
B)  Maternally 
C)  In multiples of six bases 
D)  Randomly

Question 5

Southern blot is used to detect which of the following polymorphisms?

Accepted Answer

A)  Edited RNA 
B)  Alternative splicing 
C)  Restriction fragment length polymorphisms 
D)  Modified nucleotides 
A)  Edited RNA 
B)  Alternative splicing 
C)  Restriction fragment length polymorphisms 
D)  Modified nucleotides

Question 6

Which of the following STRs is a Y-STR?

Accepted Answer

A)  D5S818 
B)  vWA 
C)  Penta E 
D)  DYS385 
A)  D5S818 
B)  vWA 
C)  Penta E 
D)  DYS385

Question 7

If a locus on chromosome 16 is homozygous, then the locus has:

Accepted Answer

A)  Many different alleles on one chromosome 16 
B)  The same alleles on each chromosome 16 homolog 
C)  Different alleles on each chromosome 16 homolog 
D)  No alleles 
A)  Many different alleles on one chromosome 16 
B)  The same alleles on each chromosome 16 homolog 
C)  Different alleles on each chromosome 16 homolog 
D)  No alleles

Question 8

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

Accepted Answer

A)  Female 
B)  Male 
C)  Impossible to tell from one locus 
D)  This locus does not discriminate between males and females. 
A)  Female 
B)  Male 
C)  Impossible to tell from one locus 
D)  This locus does not discriminate between males and females.

Question 9

THO1, D8S1179, and D2S11 are examples of which of the following?

Accepted Answer

A)  RFLP 
B)  SINE 
C)  STR 
D)  SNP 
A)  RFLP 
B)  SINE 
C)  STR 
D)  SNP

Question 10

Which of the following polymorphisms are tandem repeats of 1-8 bp?

Accepted Answer

A)  Restriction fragment length polymorphisms 
B)  Short tandem repeats 
C)  Variable number tandem repeats 
D)  Single nucleotide polymorphisms 
A)  Restriction fragment length polymorphisms 
B)  Short tandem repeats 
C)  Variable number tandem repeats 
D)  Single nucleotide polymorphisms

Question 11

How are Y alleles inherited?

Accepted Answer

A)  As recombined combinations on two chromosomes 
B)  As a diploid genotype 
C)  As a haplotype on one chromosome 
D)  Maternally 
A)  As recombined combinations on two chromosomes 
B)  As a diploid genotype 
C)  As a haplotype on one chromosome 
D)  Maternally

Question 12

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Accepted Answer

A)  vWA and TH01 
B)  TH01 and D13S317 
C)  D16S539 and F13A01 
D)  vWA and D13S317 
A)  vWA and TH01 
B)  TH01 and D13S317 
C)  D16S539 and F13A01 
D)  vWA and D13S317

Question 13

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Accepted Answer

A)  STR 
B)  Y-STR 
C)  RFLP 
D)  VNTR 
A)  STR 
B)  Y-STR 
C)  RFLP 
D)  VNTR

Question 14

Two elderly women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

Accepted Answer

A)  Y-STR 
B)  Mitochondrial DNA 
C)  RFLP-PCR 
D)  SNP haplotypes 
A)  Y-STR 
B)  Mitochondrial DNA 
C)  RFLP-PCR 
D)  SNP haplotypes

Question 15

What is a microvariant?

Accepted Answer

A)  An STR allele missing base pairs in the repeat unit 
B)  Any mini-STR allele 
C)  A viral genome integrated into host DNA 
D)  A DNA region with many HaeIII sites 
A)  An STR allele missing base pairs in the repeat unit 
B)  Any mini-STR allele 
C)  A viral genome integrated into host DNA 
D)  A DNA region with many HaeIII sites

Question 16

A woman has been sexually assaulted by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

Accepted Answer

A)  RFLP 
B)  STR 
C)  Y-STR 
D)  VNTR 
A)  RFLP 
B)  STR 
C)  Y-STR 
D)  VNTR

Question 17

Bone marrow has been transplanted into a recipient. STR analysis was performed on whole blood from the recipient 6 months after the transplant by PCR, followed by capillary gel electrophoresis with fluorescence measurement of amplicons. For the TPOX locus, the donor and recipient do not share alleles. The fluorescence of the TPOX locus for the donor peak was 15693, and the fluorescence of the TPOX locus for the recipient peak was 5329. What is the percentage of recipient cells remaining?

Accepted Answer

A)  25% 
B)  34% 
C)  66% 
D)  75% 
A)  25% 
B)  34% 
C)  66% 
D)  75%

Question 18

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

Accepted Answer

A)  To determine whether the donor and recipient are a match 
B)  To determine the likelihood of graft-versus-host disease 
C)  To find informative alleles for post-transplantation studies 
D)  To add their STR haplotypes to a national database 
A)  To determine whether the donor and recipient are a match 
B)  To determine the likelihood of graft-versus-host disease 
C)  To find informative alleles for post-transplantation studies 
D)  To add their STR haplotypes to a national database

Question 19

How do STR and VNTR differ?

Accepted Answer

A)  STR have fewer repeats than VNTR. 
B)  STR have longer repeat units than VNTR. 
C)  STR have shorter repeat units than VNTR. 
D)  STR alleles are only distinguishable by Southern blot. 
A)  STR have fewer repeats than VNTR. 
B)  STR have longer repeat units than VNTR. 
C)  STR have shorter repeat units than VNTR. 
D)  STR alleles are only distinguishable by Southern blot.

Question 20

What is an expression for how many times more likely the child's allele is inherited from the alleged father than by random occurrence of the allele in the general population?

Accepted Answer

A)  Exclusion 
B)  Paternity index 
C)  Inclusion 
D)  Allele frequency 
A)  Exclusion 
B)  Paternity index 
C)  Inclusion 
D)  Allele frequency

Mitochondrial polymorphisms used for human identification are found in what part of the mitochondrial genome?

Two peaks from different sources falling within a bin describe what relationship?

Which of the following is used as a marker of gender?

How are mitochondrial polymorphisms inherited?

Southern blot is used to detect which of the following polymorphisms?

Which of the following STRs is a Y-STR?

If a locus on chromosome 16 is homozygous, then the locus has:

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

THO1, D8S1179, and D2S11 are examples of which of the following?

Which of the following polymorphisms are tandem repeats of 1-8 bp?

How are Y alleles inherited?

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Two elderly women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

What is a microvariant?

A woman has been sexually assaulted by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

How do STR and VNTR differ?

What is an expression for how many times more likely the child's allele is inherited from the alleged father than by random occurrence of the allele in the general population?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Dna-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 11: DNA Polymorphisms and Human Identification

Mitochondrial polymorphisms used for human identification are found in what part of the mitochondrial genome?

Two peaks from different sources falling within a bin describe what relationship?

Which of the following is used as a marker of gender?

How are mitochondrial polymorphisms inherited?

Southern blot is used to detect which of the following polymorphisms?

Which of the following STRs is a Y-STR?

If a locus on chromosome 16 is homozygous, then the locus has:

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

THO1, D8S1179, and D2S11 are examples of which of the following?

Which of the following polymorphisms are tandem repeats of 1-8 bp?

How are Y alleles inherited?

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Two elderly women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

What is a microvariant?

A woman has been sexually assaulted by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

How do STR and VNTR differ?

What is an expression for how many times more likely the child's allele is inherited from the alleged father than by random occurrence of the allele in the general population?

DNA

RNA

Proteins

Nucleic Acid Extraction Methods

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Dna-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters