Exam 6: Analysis and Characterization of Nucleic Acids and Proteins

A Southern blot procedure has been performed, but the molecular weight markers are the only bands seen on the gel. Apparently, the probe did not bind to its target on the membrane. How might the stringency be adjusted so that the probe will bind?

What is learned from the size of restriction fragments as determined by migration speed in gel electrophoresis?

In order to depurinate DNA after electrophoresis, the gel should be soaked in which of the following?

What is the melting temperature (T_m) of the following hybrid: AGCTATGCCGGCTAGCAC TCGATACGGCCGATCGTG

What is the next step in the Southern blot procedure after restriction enzyme digestion?

Which of the following bind(s) specifically to a sequence of interest, thus facilitating the analysis of that sequence?

The specificity of western blots depends on what interaction?

The microarray or chip is an example of which of the following blotting procedures?

The melting temperature of nucleic acid is the temperature when:

Which of the following conditions are the least stringent?

What determines the specificity in terms of the gene or region that is detected in the Southern blot?

A circular molecule with one restriction enzyme recognition site will yield how many fragments when digested by the restriction enzyme?

A circular molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

What is star activity?

A linear molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?

Which of the following molecules comprises most membranes that bind to nucleic acids in blotting procedures?

What was the great advantage to molecular analysis offered by Southern blot?

What process facilitates denaturation of long DNA fragments?

Which of the following solutions has the highest stringency?

Which of the following methods is best suited for qualitative analysis of DNA, such as mutational analysis, where many samples are compared simultaneously?