Question 1

Which of the following will yield the best DNA preparation?

Accepted Answer

A)  Fixed tissue 
B)  Hemolyzed blood 
C)  Fresh blood 
D)  Stool 
A)  Fixed tissue 
B)  Hemolyzed blood 
C)  Fresh blood 
D)  Stool C

Question 2

What is the yield of DNA from a sample with concentration of 280 F1F1F1S1F1F1F10g/mL and a volume of 0.5 mL?

Accepted Answer

A)  70 F1F1F1S1F1F1F10g 
B)  140 F1F1F1S1F1F1F10g 
C)  280 F1F1F1S1F1F1F10g 
D)  560 F1F1F1S1F1F1F10g 
A)  70 F1F1F1S1F1F1F10g 
B)  140 F1F1F1S1F1F1F10g 
C)  280 F1F1F1S1F1F1F10g 
D)  560 F1F1F1S1F1F1F10g B

Question 3

Which of the following is used to optimize yield of nucleic acid by precipitation or column purification?

Accepted Answer

A)  Carrier molecules 
B)  Detergent 
C)  Tris buffer 
D)  Isotonic saline 
A)  Carrier molecules 
B)  Detergent 
C)  Tris buffer 
D)  Isotonic saline A

Question 4

DNA is isolated from a clinical sample. The absorbance at 260 nm is 0.489, and the absorbance at 280 nm is 0.257. Is this sample of sufficient quality for use in subsequent analyses?

Accepted Answer

A)  Yes 
B)  No 
C)  Cannot be determined with the data given 
A)  Yes 
B)  No 
C)  Cannot be determined with the data given

Question 5

What is the concentration of DNA whereby a 1:100 dilution has an absorbance reading of 0.015 at 260 nm?

Accepted Answer

A)  6 F1F1F1S1F1F1F10g/mL 
B)  60 F1F1F1S1F1F1F10g/mL 
C)  75 F1F1F1S1F1F1F10g/mL 
D)  750 F1F1F1S1F1F1F10g/mL 
A)  6 F1F1F1S1F1F1F10g/mL 
B)  60 F1F1F1S1F1F1F10g/mL 
C)  75 F1F1F1S1F1F1F10g/mL 
D)  750 F1F1F1S1F1F1F10g/mL

Question 6

Nucleic acid concentration can be assessed relatively simply and quickly by which of the following procedures?

Accepted Answer

A)  Electrophoresis 
B)  Enzyme immunoassay 
C)  Sequencing 
D)  Spectrophotometry at 260 nm 
A)  Electrophoresis 
B)  Enzyme immunoassay 
C)  Sequencing 
D)  Spectrophotometry at 260 nm

Question 7

Phenol/chloroform and an aqueous solution will form what type of mixture?

Accepted Answer

A)  Simple solution 
B)  Emulsion 
C)  Suspension 
D)  Supersaturated solution 
A)  Simple solution 
B)  Emulsion 
C)  Suspension 
D)  Supersaturated solution

Question 8

In inorganic DNA isolation, or "salting out" procedures, in the presence of low-pH and high-salt concentrations, which intracellular component precipitates out of solution?

Accepted Answer

A)  DNA 
B)  RNA 
C)  Carbohydrate 
D)  Protein 
A)  DNA 
B)  RNA 
C)  Carbohydrate 
D)  Protein

Question 9

Diethyl pyrocarbonate (DEPC) is a chemical that is used to:

Accepted Answer

A)  Inactivate DNAses 
B)  Measure DNA concentration 
C)  Isolate mitochondria 
D)  Inactivate RNAses 
A)  Inactivate DNAses 
B)  Measure DNA concentration 
C)  Isolate mitochondria 
D)  Inactivate RNAses

Question 10

The 260 nm/280 nm ratio for isolated DNA was determined to be 1:2. What should be done next with this sample?

Accepted Answer

A)  Proceed with DNA analysis as necessary. 
B)  Reprecipitate the DNA to remove excess protein. 
C)  Treat the sample with RNAse to remove RNA. 
D)  Dilute the sample to 1:10 and repeat spectrophotometry. 
A)  Proceed with DNA analysis as necessary. 
B)  Reprecipitate the DNA to remove excess protein. 
C)  Treat the sample with RNAse to remove RNA. 
D)  Dilute the sample to 1:10 and repeat spectrophotometry.

Question 11

Which whole blood fraction is the most abundant source of genomic DNA?

Accepted Answer

A)  Red blood cells 
B)  Buffy coat 
C)  Serum 
D)  Plasma 
A)  Red blood cells 
B)  Buffy coat 
C)  Serum 
D)  Plasma

Question 12

DNA, after separation from other cellular constituents, is precipitated in which of the following?

Accepted Answer

A)  Chloroform 
B)  Isopropanol 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium dodecyl sulfate 
A)  Chloroform 
B)  Isopropanol 
C)  Ethylenediaminetetraacetic acid 
D)  Sodium dodecyl sulfate

Question 13

In organic isolation of RNA procedures, guanidinium isothiocyanate is added to:

Accepted Answer

A)  Degrade DNA 
B)  Denature all proteins 
C)  Inhibit RNAses 
D)  Separate RNA from DNA 
A)  Degrade DNA 
B)  Denature all proteins 
C)  Inhibit RNAses 
D)  Separate RNA from DNA

Question 14

Which of the following is the first step in DNA isolation from cells in a clinical sample?

Accepted Answer

A)  DNA precipitation 
B)  Precipitation of proteins 
C)  Lysis of cells 
D)  Eluting DNA from a spin column 
A)  DNA precipitation 
B)  Precipitation of proteins 
C)  Lysis of cells 
D)  Eluting DNA from a spin column

Question 15

The most abundant form of RNA in all cells is:

Accepted Answer

A)  Ribosomal 
B)  Transfer 
C)  Messenger 
D)  Small nuclear 
A)  Ribosomal 
B)  Transfer 
C)  Messenger 
D)  Small nuclear

Question 16

Which of the following will not inhibit RNAses?

Accepted Answer

A)  Guanidine isothiocyanate (GIT
C)  
B)  Tris buffer 
C)  Strong reducing agents 
D)  High-salt buffers 
A)  Guanidine isothiocyanate (GIT
C)  
B)  Tris buffer 
C)  Strong reducing agents 
D)  High-salt buffers

Question 17

What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.675 at 260 nm?

Accepted Answer

A)  33.75 F1F1F1S1F1F1F10g/mL 
B)  337.5 F1F1F1S1F1F1F10g/mL 
C)  27 F1F1F1S1F1F1F10g/mL 
D)  270 F1F1F1S1F1F1F10g/mL 
A)  33.75 F1F1F1S1F1F1F10g/mL 
B)  337.5 F1F1F1S1F1F1F10g/mL 
C)  27 F1F1F1S1F1F1F10g/mL 
D)  270 F1F1F1S1F1F1F10g/mL

Question 18

When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of:

Accepted Answer

A)  20 
B)  30 
C)  40 
D)  50 
A)  20 
B)  30 
C)  40 
D)  50

Question 19

What is the yield of RNA from the sample with concentration of 450 F1F1F1S1F1F1F10g/mL and a volume of 0.3 mL?

Accepted Answer

A)  135 F1F1F1S1F1F1F10g 
B)  150 F1F1F1S1F1F1F10g 
C)  225 F1F1F1S1F1F1F10g 
D)  450 F1F1F1S1F1F1F10g 
A)  135 F1F1F1S1F1F1F10g 
B)  150 F1F1F1S1F1F1F10g 
C)  225 F1F1F1S1F1F1F10g 
D)  450 F1F1F1S1F1F1F10g

Question 20

In what way is fluorometry more accurate than spectrophotometry?

Accepted Answer

A)  Fluorescence is more specific for single nucleotides. 
B)  Spectrophotometry only measures absorbance from intact nucleic acid polymers. 
C)  Using specific stains, fluorometry only detects intact double-stranded DNA. 
D)  Absorbance may require dilution of sample before reading. 
A)  Fluorescence is more specific for single nucleotides. 
B)  Spectrophotometry only measures absorbance from intact nucleic acid polymers. 
C)  Using specific stains, fluorometry only detects intact double-stranded DNA. 
D)  Absorbance may require dilution of sample before reading.

Which of the following will yield the best DNA preparation?

What is the yield of DNA from a sample with concentration of 280 g/mL and a volume of 0.5 mL?

Which of the following is used to optimize yield of nucleic acid by precipitation or column purification?

DNA is isolated from a clinical sample. The absorbance at 260 nm is 0.489, and the absorbance at 280 nm is 0.257. Is this sample of sufficient quality for use in subsequent analyses?

What is the concentration of DNA whereby a 1:100 dilution has an absorbance reading of 0.015 at 260 nm?

Nucleic acid concentration can be assessed relatively simply and quickly by which of the following procedures?

Phenol/chloroform and an aqueous solution will form what type of mixture?

In inorganic DNA isolation, or "salting out" procedures, in the presence of low-pH and high-salt concentrations, which intracellular component precipitates out of solution?

Diethyl pyrocarbonate (DEPC) is a chemical that is used to:

The 260 nm/280 nm ratio for isolated DNA was determined to be 1:2. What should be done next with this sample?

Which whole blood fraction is the most abundant source of genomic DNA?

DNA, after separation from other cellular constituents, is precipitated in which of the following?

In organic isolation of RNA procedures, guanidinium isothiocyanate is added to:

Which of the following is the first step in DNA isolation from cells in a clinical sample?

The most abundant form of RNA in all cells is:

Which of the following will not inhibit RNAses?

What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.675 at 260 nm?

When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of:

What is the yield of RNA from the sample with concentration of 450 g/mL and a volume of 0.3 mL?

In what way is fluorometry more accurate than spectrophotometry?

DNA

RNA

Proteins

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Dna-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters

Exam 4: Nucleic Acid Extraction Methods

Which of the following will yield the best DNA preparation?

What is the yield of DNA from a sample with concentration of 280 g/mL and a volume of 0.5 mL?

Which of the following is used to optimize yield of nucleic acid by precipitation or column purification?

DNA is isolated from a clinical sample. The absorbance at 260 nm is 0.489, and the absorbance at 280 nm is 0.257. Is this sample of sufficient quality for use in subsequent analyses?

What is the concentration of DNA whereby a 1:100 dilution has an absorbance reading of 0.015 at 260 nm?

Nucleic acid concentration can be assessed relatively simply and quickly by which of the following procedures?

Phenol/chloroform and an aqueous solution will form what type of mixture?

In inorganic DNA isolation, or "salting out" procedures, in the presence of low-pH and high-salt concentrations, which intracellular component precipitates out of solution?

Diethyl pyrocarbonate (DEPC) is a chemical that is used to:

The 260 nm/280 nm ratio for isolated DNA was determined to be 1:2. What should be done next with this sample?

Which whole blood fraction is the most abundant source of genomic DNA?

DNA, after separation from other cellular constituents, is precipitated in which of the following?

In organic isolation of RNA procedures, guanidinium isothiocyanate is added to:

Which of the following is the first step in DNA isolation from cells in a clinical sample?

The most abundant form of RNA in all cells is:

Which of the following will not inhibit RNAses?

What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.675 at 260 nm?

When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of:

What is the yield of RNA from the sample with concentration of 450 g/mL and a volume of 0.3 mL?

In what way is fluorometry more accurate than spectrophotometry?

DNA

RNA

Proteins

Resolution and Detection of Nucleic Acids

Analysis and Characterization of Nucleic Acids and Proteins

Nucleic Acid Amplification

Chromosomal Structure and Chromosomal Mutations

Gene Mutations

DNA Sequencing

DNA Polymorphisms and Human Identification

Detection and Identification of Microorganisms

Molecular Detection of Inherited Diseases

Molecular Oncology

Dna-Based Tissue Typing

Quality Assurance and Quality Control in the Molecular Laboratory

Filters