Exam 10: DNA Sequencing

In the Sanger sequencing method, which of the following when incorporated into the growing strand are used to determine the identity of the base at a particular position in a piece of DNA?

Dye terminator sequencing has been performed using capillary electrophoresis to separate the fragments. ddATP has been labeled with a "green" fluor, ddTTP with "red," ddCTP with "blue," and ddGTP with "black." The colors are detected in the following order: red, green, black, red, green, blue, red, black, black, green, blue. What is the sequence of this fragment?

The ddNTP:dNTP ratio is too high (too much ddNTP). How will this affect sequencing results?

Dideoxycytidine is added to one of four sequencing reactions. All of the DNA fragments produced in that reaction end with which of the following nucleotides?

Which end of the sequenced DNA is found at the bottom of a sequencing gel?

Sequencing ladders resolved by gel electrophoresis are read in which of the following directions?

Which of the following pieces of information has been determined about the human genome?

Which of the following is a genetic sequence database that is sponsored by the National Institutes of Health?

In Maxam-Gilbert sequencing, a band that is present in the lane from the tube treated with formic acid (G or A) but not the tube treated with dimethyl sulfate (G) is read as which of the following nucleotides?

Cycle sequencing was made possible through the development of what reagents?

A segment of DNA has been sequenced and noted as the following: AYKWHT. Which of the following is the correct interpretation of this notation?

Ten equivalents of a genome were cut into small pieces and sequenced. Computers were then used to put the sequences of the pieces together to determine the sequence of the intact genome. The method described is which of the following?

Which of the following is the most widely used method of DNA sequencing in clinical laboratories today?

A Sanger sequencing procedure has been performed on a 300-bp piece of DNA. When the fragments were resolved on the gel, there were bands distributed throughout the T, C and A lanes, but in the G lane, there was one thick band at the bottom of the gel. What is the likely cause of these results?

A pyrosequencer detects visible light, making it which type of instrument?

In Maxam-Gilbert sequencing, co-migrating bands that are present in lanes from the tube treated with hydrazine (T or C) and the tube treated with hydrazine plus salt (T) are read as which of the following nucleotides?

An analysis that takes place in silico is happening in which of the following places?

When two sequences are found to be exactly the same, they have which of the following?

A portion of a sequencing gel is described as follows: the first band at the bottom of the gel was in the ddATP lane; the next band going up the gel was in the ddTTP lane, then the ddCTP lane, the ddGTP lane, the ddATP lane, the ddTTP lane, the ddCTP lane, the ddCTP lane, the ddATP lane, and the ddGTP lane. Which of the following is the correct sequence as determined on this gel?

What part of the human genome accounts for encoded proteins?