Question 1

Restriction enzymes are:

Accepted Answer

A)  DNA polymerases 
B)  RNA polymerases 
C)  endonucleases 
D)  exonucleases 
E)  none of the above 
A)  DNA polymerases 
B)  RNA polymerases 
C)  endonucleases 
D)  exonucleases 
E)  none of the above

Question 2

Recombination does not

Accepted Answer

A)  play a role in the repair of DNA. 
B)  generate new combinations of genes. 
C)  generate point mutations. 
D)  regulate the expression of DNA. 
E)  involve single strand breaks. 
A)  play a role in the repair of DNA. 
B)  generate new combinations of genes. 
C)  generate point mutations. 
D)  regulate the expression of DNA. 
E)  involve single strand breaks.

Question 3

Alternative splicing

Accepted Answer

A)  gives rise to different arrangements of genomic DNA. 
B)  results in the production of distinct proteins having a number of domains in common. 
C)  requires different U1 snRNAs. 
D)  results from the actions of different RNases to degrade exon sequences. 
E)  involves different proteolytic processing events to generate different proteins. 
A)  gives rise to different arrangements of genomic DNA. 
B)  results in the production of distinct proteins having a number of domains in common. 
C)  requires different U1 snRNAs. 
D)  results from the actions of different RNases to degrade exon sequences. 
E)  involves different proteolytic processing events to generate different proteins.

Question 4

Knockout mice are characterized by all of the following EXCEPT

Accepted Answer

A)  They contain null mutations in mouse genes. 
B)  They are generated by homologous recombination of a gene targeting vector in embryonic stem cells. 
C)  The initial mice produced are chimeric. 
D)  The effects of the mutations are usually pleiotropic. 
E)  The mutations are usually lethal during the embryonic stage of development. 
A)  They contain null mutations in mouse genes. 
B)  They are generated by homologous recombination of a gene targeting vector in embryonic stem cells. 
C)  The initial mice produced are chimeric. 
D)  The effects of the mutations are usually pleiotropic. 
E)  The mutations are usually lethal during the embryonic stage of development.

Question 5

Each of the following statements concerning reverse transcriptase is true EXCEPT?

Accepted Answer

A)  It has been found as a component of RNA tumor viruses. 
B)  It can use an RNA template for the synthesis of DNA. 
C)  It can be used for the construction of cDNA clones from mRNA. 
D)  It carries out the transcription of viral_specific mRNAs. 
E)  It can utilize phosphorylated AZT for nucleic acid synthesis. 
A)  It has been found as a component of RNA tumor viruses. 
B)  It can use an RNA template for the synthesis of DNA. 
C)  It can be used for the construction of cDNA clones from mRNA. 
D)  It carries out the transcription of viral_specific mRNAs. 
E)  It can utilize phosphorylated AZT for nucleic acid synthesis.

Question 6

Construction of a plasmid containing a human beta globin cDNA may involve all of the following EXCEPT

Accepted Answer

A)  isolation of human beta globin mRNA. 
B)  use of poly dT as a primer for reverse transcriptase. 
C)  transcription with 
D)  homopolymer tailing of the cDNA and ligation of the tailed cDNA to the vector. 
E)  coli RNA polymerase. 
A)  isolation of human beta globin mRNA. 
B)  use of poly dT as a primer for reverse transcriptase. 
C)  transcription with 
D)  homopolymer tailing of the cDNA and ligation of the tailed cDNA to the vector. 
E)  coli RNA polymerase.

Question 7

Reverse transcriptase and terminal deoxynucleotidyl transferase are used in gene cloning to

Accepted Answer

A)  remove single stranded DNA and repair the restriction enzyme site after transformation respectively. 
B)  make cDNA and create sticky ends on the cDNA and the staggered ends of the cleaved restriction site. 
C)  repair the restriction enzyme site before transformation. 
D)  repair the restriction enzyme site after transformation. 
E)  remove the sticky ends prior to the DNA ligase step. 
A)  remove single stranded DNA and repair the restriction enzyme site after transformation respectively. 
B)  make cDNA and create sticky ends on the cDNA and the staggered ends of the cleaved restriction site. 
C)  repair the restriction enzyme site before transformation. 
D)  repair the restriction enzyme site after transformation. 
E)  remove the sticky ends prior to the DNA ligase step.

Question 8

A technique that is used to determine the abundance of a particular mRNA is

Accepted Answer

A)  gel shift assay. 
B)  dideoxy chain termination. 
C)  Southern blotting. 
D)  DNA ligation. 
E)  Northern blotting. 
A)  gel shift assay. 
B)  dideoxy chain termination. 
C)  Southern blotting. 
D)  DNA ligation. 
E)  Northern blotting.

Question 9

RFLP analysis can be used for all of the following except

Accepted Answer

A)  classical genetic mapping. 
B)  DNA fingerprinting. 
C)  locating a disease gene. 
D)  prenatal screening for a possible genetic defect. 
E)  creating a cDNA library. 
A)  classical genetic mapping. 
B)  DNA fingerprinting. 
C)  locating a disease gene. 
D)  prenatal screening for a possible genetic defect. 
E)  creating a cDNA library.

Question 10

Techniques utilized to produce mouse models of human disease include all of the following EXCEPT

Accepted Answer

A)  knock-in mutation. 
B)  transgenesis. 
C)  nuclear transfer. 
D)  transposition. 
E)  knockout mutation. 
A)  knock-in mutation. 
B)  transgenesis. 
C)  nuclear transfer. 
D)  transposition. 
E)  knockout mutation.

Question 11

Human reproductive cloning, therapeutic cloning, nuclear transfer and nuclear transplantation are all variations of the same technique in which

Accepted Answer

A)  in utero gene transfer is performed. 
B)  transgenes are microinjected into embryonic stem cells. 
C)  the nucleus of a somatic cell is introduced into the cytoplasm of an enucleated cell. 
D)  homologous recombination is used to achieve new traits. 
A)  in utero gene transfer is performed. 
B)  transgenes are microinjected into embryonic stem cells. 
C)  the nucleus of a somatic cell is introduced into the cytoplasm of an enucleated cell. 
D)  homologous recombination is used to achieve new traits.

Question 12

DNA sequencing by the Sanger technique makes use of ____________for chain termination.

Accepted Answer

A)  dNTPs 
B)  dideoxynucleotides 
C)  AZT 
D)  DNA polymerase 
A)  dNTPs 
B)  dideoxynucleotides 
C)  AZT 
D)  DNA polymerase

Question 13

Which one of the following properties would make a plasmid an ineffective vehicle?

Accepted Answer

A)  very small size 
B)  multiple copies are incorporated per cell 
C)  codes for the protein required for tetracycline resistance 
D)  contains three sites for the restriction endonuclease to be used 
A)  very small size 
B)  multiple copies are incorporated per cell 
C)  codes for the protein required for tetracycline resistance 
D)  contains three sites for the restriction endonuclease to be used

Question 14

Screening a recombinant DNA library using an 125I-labeled antibody prepared against a eukaryotic protein

Accepted Answer

A)  requires that cDNA inserts have been used in the library being screened. 
B)  requires the utilization of vector sequences that provide high efficiency translation of the cloned insert DNA including signals for initiation and termination of translation. 
C)  requires the utilization of vector sequences that provide high efficiency transcription of the cloned insert DNA including signals for initiation and termination of transcription. 
D)  will isolate any gene that encodes a protein product recognized by the antibody. 
E)  all of the above statements are true. 
A)  requires that cDNA inserts have been used in the library being screened. 
B)  requires the utilization of vector sequences that provide high efficiency translation of the cloned insert DNA including signals for initiation and termination of translation. 
C)  requires the utilization of vector sequences that provide high efficiency transcription of the cloned insert DNA including signals for initiation and termination of transcription. 
D)  will isolate any gene that encodes a protein product recognized by the antibody. 
E)  all of the above statements are true.

Question 15

In Southern blot hybridization analysis

Accepted Answer

A)  reverse transcriptase is used to synthesize a radioactive nucleic acid probe 
B)  nick translation is used to obtain a protein-derived probe. 
C)  DNA is denatured and lifted from the agar surface with a nitrocellulose filter. 
D)  restriction fragments containing a specific nucleotide sequence can be identified. 
E)  DNA fragments are resolved on a CsCl density gradient. 
A)  reverse transcriptase is used to synthesize a radioactive nucleic acid probe 
B)  nick translation is used to obtain a protein-derived probe. 
C)  DNA is denatured and lifted from the agar surface with a nitrocellulose filter. 
D)  restriction fragments containing a specific nucleotide sequence can be identified. 
E)  DNA fragments are resolved on a CsCl density gradient.

Question 16

To obtain recombinant DNA clones that encode a specific protein, a high affinity antibody to that protein can be utilized to screen

Accepted Answer

A)  a cDNA library in pBR. 
B)  a cDNA library in an expression vector. 
C)  a genomic DNA library cloned into a YAC vector. 
D)  a cDNA library cloned into a YAC vector. 
E)  a genomic DNA library cloned into a phage vector. 
A)  a cDNA library in pBR. 
B)  a cDNA library in an expression vector. 
C)  a genomic DNA library cloned into a YAC vector. 
D)  a cDNA library cloned into a YAC vector. 
E)  a genomic DNA library cloned into a phage vector.

Question 17

Which of the following sequences would NOT be represented in a cDNA library?

Accepted Answer

A)  promoter sequences 
B)  translation termination codons 
C)  polyadenylation signals 
D)  translation initiation codons 
A)  promoter sequences 
B)  translation termination codons 
C)  polyadenylation signals 
D)  translation initiation codons

Question 18

In the preparation of labeled probes, which steps do nick-translation and random oligonucleotide priming have in common?

Accepted Answer

A)  Labeling reactions are initiated by synthetic oligonucleotides. 
B)  The incorporation of labeled precursor involves an in vitro DNA replication reaction. 
C)  Nuclease activity can degrade newly synthesized, labeled DNA. 
D)  They both incorporate -labeled ribonucleotides. 
A)  Labeling reactions are initiated by synthetic oligonucleotides. 
B)  The incorporation of labeled precursor involves an in vitro DNA replication reaction. 
C)  Nuclease activity can degrade newly synthesized, labeled DNA. 
D)  They both incorporate -labeled ribonucleotides.

Question 19

Two general types of viral vectors utilized frequently for gene therapy include.

Accepted Answer

A)  bacteriophage and HIV 
B)  DNA viruses and RNA viruses 
C)  adenoviruses and foamy viruses 
D)  influenza viruses and liposomes 
A)  bacteriophage and HIV 
B)  DNA viruses and RNA viruses 
C)  adenoviruses and foamy viruses 
D)  influenza viruses and liposomes

Question 20

The degree of homology between DNA from two organisms can most accurately be determined by

Accepted Answer

A)  base composition. 
B)  comparing melting curves. 
C)  density measurements. 
D)  hybridization. 
A)  base composition. 
B)  comparing melting curves. 
C)  density measurements. 
D)  hybridization.

Exam 7: Recombinant Dna and Biotechnology

Restriction enzymes are:

Recombination does not

Alternative splicing

Knockout mice are characterized by all of the following EXCEPT

Each of the following statements concerning reverse transcriptase is true EXCEPT?

Construction of a plasmid containing a human beta globin cDNA may involve all of the following EXCEPT

Reverse transcriptase and terminal deoxynucleotidyl transferase are used in gene cloning to

A technique that is used to determine the abundance of a particular mRNA is

RFLP analysis can be used for all of the following except

Techniques utilized to produce mouse models of human disease include all of the following EXCEPT

Human reproductive cloning, therapeutic cloning, nuclear transfer and nuclear transplantation are all variations of the same technique in which

DNA sequencing by the Sanger technique makes use of ____________for chain termination.

Which one of the following properties would make a plasmid an ineffective vehicle?

Screening a recombinant DNA library using an 125I-labeled antibody prepared against a eukaryotic protein

In Southern blot hybridization analysis

To obtain recombinant DNA clones that encode a specific protein, a high affinity antibody to that protein can be utilized to screen

Which of the following sequences would NOT be represented in a cDNA library?

In the preparation of labeled probes, which steps do nick-translation and random oligonucleotide priming have in common?

Two general types of viral vectors utilized frequently for gene therapy include.

The degree of homology between DNA from two organisms can most accurately be determined by

Eukaryotic Cell Structure

DNA and RNA: Composition and Structure

Protein I: Composition and Structure

DNA Replication, Recombination, and Repair

RNA: Transcription and Rna Processing

Protein Synthesis: Translation and Posttranslational Modifications

Regulation of Gene Expression

Protein II: Structure-Function Relationships in Protein Families

Enzymes: Classification, Kinetics, and Control

The Cytochromes P450 and Nitric Oxide Synthases

Biological Membranes: Structure and Membrane Transport

Fundamentals of Signal Transduction

Bioenergetics and Oxidative Metabolism

Carbohydrate Metabolism I: Major Metabolic Pathways and Their Control

Carbohydrate Metabolism II: Special Pathways and Glycoconjugates

Lipid Metabolism I: Synthesis, Storage, and Utilization of Fatty Acids and Triacylglycerols

Lipid Metabolism II: Pathways of Metabolism of Special Lipids

Amino Acid Metabolism

Purine and Pyrimidine Nucleotide Metabolism

Metabolic Interrelationships

Biochemistry of Hormones

Molecular Cell Biology

Cell Cycle, Programmed Cell Death, and Cancer

Digestion and Absorption of Basic Nutritional Constituents

Digestion and Absorption of Basic Nutritional Constituents

Principles of Nutrition I and II

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