Question 1

Which of the following is required for constructing a cDNA library?

Accepted Answer

A)  a genomic clone of the gene of interest. 
B)  Purified RNA polymerase 
C)  RNA_dependent DNA polymerase. 
D)  Antibody raised against the protein encoded by the mRNA of interest. 
A)  a genomic clone of the gene of interest. 
B)  Purified RNA polymerase 
C)  RNA_dependent DNA polymerase. 
D)  Antibody raised against the protein encoded by the mRNA of interest.

Question 2

Transgenic mouse lines are useful for studies of human disease for all the following reasons EXCEPT

Accepted Answer

A)  Mice are physiologically similar to humans. 
B)  New diseases can be induced in mice for which there is no human counterpart yet. 
C)  They allow the study of polygenic disease. 
D)  Technology advances have increased the ability to create mouse models of human disease. 
E)  A large reservoir of potential models exists in spontaneous or induced mutant loci. 
A)  Mice are physiologically similar to humans. 
B)  New diseases can be induced in mice for which there is no human counterpart yet. 
C)  They allow the study of polygenic disease. 
D)  Technology advances have increased the ability to create mouse models of human disease. 
E)  A large reservoir of potential models exists in spontaneous or induced mutant loci.

Question 3

Allele-specific oligonucleotide (ASO) analysis is used primarily for

Accepted Answer

A)  identification of point mutations. 
B)  detection of deleted exons. 
C)  linkage analysis. 
D)  detection of trinucleotide repeat expansions. 
A)  identification of point mutations. 
B)  detection of deleted exons. 
C)  linkage analysis. 
D)  detection of trinucleotide repeat expansions.

Question 4

Segments of pure samples of identical, relatively short DNA molecules from plasmids can be subdivided into smaller pieces by

Accepted Answer

A)  Southern blot analysis. 
B)  colony hybridization. 
C)  restriction enzyme digestion. 
D)  agarose gel electrophoresis. 
E)  ethidium bromide treatment. 
A)  Southern blot analysis. 
B)  colony hybridization. 
C)  restriction enzyme digestion. 
D)  agarose gel electrophoresis. 
E)  ethidium bromide treatment.

Question 5

Eukaryotic chromosomes are characterized by each of the following, EXCEPT

Accepted Answer

A)  contain negatively supercoiled DNA. 
B)  consist of linear DNA polynucleotide chains. 
C)  contain both repetitive and nonrepetitive DNA. 
D)  contain nucleosomes spaced approximately 200 bp apart. 
E)  contain restriction enzymes. 
A)  contain negatively supercoiled DNA. 
B)  consist of linear DNA polynucleotide chains. 
C)  contain both repetitive and nonrepetitive DNA. 
D)  contain nucleosomes spaced approximately 200 bp apart. 
E)  contain restriction enzymes.

Question 6

For an expression vector that will be used to produce mRNA in a prokaryotic system, which of the following is not required?

Accepted Answer

A)  origin of replication. 
B)  selectable marker. 
C)  bacterial promoter. 
D)  polyadenylation signal. 
E)  Shine_Dalgarno sequence. 
A)  origin of replication. 
B)  selectable marker. 
C)  bacterial promoter. 
D)  polyadenylation signal. 
E)  Shine_Dalgarno sequence.

Question 7

Which stage in the process of gene cloning are the restriction endonucleases utilized?

Accepted Answer

A)  isolation of mRNA 
B)  synthesis of double-stranded cDNA 
C)  insertion of the cDNA into the vehicle 
D)  transformation 
E)  expression of the cDNA in the bacterial cell 
A)  isolation of mRNA 
B)  synthesis of double-stranded cDNA 
C)  insertion of the cDNA into the vehicle 
D)  transformation 
E)  expression of the cDNA in the bacterial cell

Question 8

Southern blots are used to analyze DNA that has been fractionated based on difference in

Accepted Answer

A)  length. 
B)  sequence. 
C)  GC content. 
D)  AT content. 
E)  density. 
A)  length. 
B)  sequence. 
C)  GC content. 
D)  AT content. 
E)  density.

Question 9

The Holliday model of general recombination provides for

Accepted Answer

A)  exchange of genetic information between nonhomologous chromosomes. 
B)  reciprocal translocation of unlike DNA between sister chromatids. 
C)  conversion of one allele to another by DNA repair. 
D)  exchange of genetic information between homologous chromosomes. 
A)  exchange of genetic information between nonhomologous chromosomes. 
B)  reciprocal translocation of unlike DNA between sister chromatids. 
C)  conversion of one allele to another by DNA repair. 
D)  exchange of genetic information between homologous chromosomes.

Question 10

Recombinant DNA techniques currently can be used for all of the following except

Accepted Answer

A)  preparation of large amounts of DNA for structural analysis. 
B)  preparation of proteins such as alpha globin, insulin, and interferon. 
C)  creation of phage collections which, in total, represent the entire genome of an organism. 
D)  prenatal identification of some genetic diseases. 
E)  repair of genetic damage caused by radiation. 
A)  preparation of large amounts of DNA for structural analysis. 
B)  preparation of proteins such as alpha globin, insulin, and interferon. 
C)  creation of phage collections which, in total, represent the entire genome of an organism. 
D)  prenatal identification of some genetic diseases. 
E)  repair of genetic damage caused by radiation.

Question 11

Which of the following are not required for expression of a human cDNA cloned in

Accepted Answer

A)  promoter 
B)  ribosome binding site 
C)  initiation codon 
D)  DNA-dependent RNA polymerase II 
E)  coli? 
A)  promoter 
B)  ribosome binding site 
C)  initiation codon 
D)  DNA-dependent RNA polymerase II 
E)  coli?

Question 12

Reverse transcriptase carries out all of the following activities except?

Accepted Answer

A)  RNA directed DNA synthesis. 
B)  DNA directed DNA synthesis. 
C)  DNA directed RNA synthesis. 
D)  Hydrolysis of RNA. 
A)  RNA directed DNA synthesis. 
B)  DNA directed DNA synthesis. 
C)  DNA directed RNA synthesis. 
D)  Hydrolysis of RNA.

Question 13

It is sometimes possible to design a degenerate oligonucleotide probe for screening a DNA library if the amino acid sequence of the protein is available. These probes are

Accepted Answer

A)  composed of a mixture of different oligonucleotide sequences. 
B)  composed only of the most frequently used codon for each amino acid. 
C)  completely random oligonucleotide sequences. 
D)  small restriction enzyme fragments. 
A)  composed of a mixture of different oligonucleotide sequences. 
B)  composed only of the most frequently used codon for each amino acid. 
C)  completely random oligonucleotide sequences. 
D)  small restriction enzyme fragments.

Question 14

Reverse transcriptase catalyzes which of the following reactions?

Accepted Answer

A)  synthesis of ribosomal RNA from pre-existing ribosomal RNA 
B)  synthesis of RNA primers for Okazaki fragments 
C)  synthesis of RNA using a single stranded RNA template 
D)  synthesis of mRNA from double stranded viral RNA 
E)  synthesis of DNA using a RNA template 
A)  synthesis of ribosomal RNA from pre-existing ribosomal RNA 
B)  synthesis of RNA primers for Okazaki fragments 
C)  synthesis of RNA using a single stranded RNA template 
D)  synthesis of mRNA from double stranded viral RNA 
E)  synthesis of DNA using a RNA template

Question 15

For an expression vector that will be used to produce mRNA in a prokaryotic system, which of the following is not required?

Accepted Answer

A)  origin of replication 
B)  selectable marker 
C)  bacterial promoter 
D)  polyadenylation signal 
E)  Shine-Dalgarno sequence 
A)  origin of replication 
B)  selectable marker 
C)  bacterial promoter 
D)  polyadenylation signal 
E)  Shine-Dalgarno sequence

Question 16

A technique that is used to determine the abundance of a particular mRNA is

Accepted Answer

A)  gel shift assay. 
B)  dideoxy chain termination. 
C)  Southern blotting. 
D)  DNA ligation. 
E)  Northern blotting. 
A)  gel shift assay. 
B)  dideoxy chain termination. 
C)  Southern blotting. 
D)  DNA ligation. 
E)  Northern blotting.

Question 17

M17482Reverse transcriptase is employed in cDNA cloning as a

Accepted Answer

A)  terminal transferase. 
B)  ligase. 
C)  RNA-dependent DNA polymerase. 
D)  DNA-dependent RNA polymerase. 
E)  kinase. 
A)  terminal transferase. 
B)  ligase. 
C)  RNA-dependent DNA polymerase. 
D)  DNA-dependent RNA polymerase. 
E)  kinase.

Question 18

Attenuation is dependent upon all of the following EXCEPT

Accepted Answer

A)  direct physical interaction between the ribosome and RNA polymerase. 
B)  pausing of a ribosome in the leader peptide due to low levels of an operon-specific charged tRNA. 
C)  no proteins other than those in ribosomes and RNA polymerase. 
D)  the formation of a stem loop structure in the nascent mRNA strand that acts as a transcriptional terminator. 
A)  direct physical interaction between the ribosome and RNA polymerase. 
B)  pausing of a ribosome in the leader peptide due to low levels of an operon-specific charged tRNA. 
C)  no proteins other than those in ribosomes and RNA polymerase. 
D)  the formation of a stem loop structure in the nascent mRNA strand that acts as a transcriptional terminator.

Question 19

Which of the following enzymes can be used for the construction of a recombinant DNA molecule in vitro?

Accepted Answer

A)  DNA polymerase + DNA ligase 
B)  DNA polymerase + RNA polymerase 
C)  Restriction enzyme + DNA ligase 
D)  Phosphorylase + DNA ligase 
E)  Acetylase + single stranded DNA specific deoxyribonuclease 
A)  DNA polymerase + DNA ligase 
B)  DNA polymerase + RNA polymerase 
C)  Restriction enzyme + DNA ligase 
D)  Phosphorylase + DNA ligase 
E)  Acetylase + single stranded DNA specific deoxyribonuclease

Question 20

Accessibility of DNA to trans-acting molecules is, in general, reduced by its being packaged into a nucleosome core, both in vitro and in vivo. A prominent exception to this is the behavior of:

Accepted Answer

A)  The TATA box-binding protein, TBP, which binds within the minor groove. 
B)  Retrovirus proviral DNA, which integrates within the major groove. 
C)  Topoisomerase II, which binds to the sugar-phosphate backbone. 
D)  SV40 large T-antigen, which binds to the major groove. 
E)  None of the above. 
A)  The TATA box-binding protein, TBP, which binds within the minor groove. 
B)  Retrovirus proviral DNA, which integrates within the major groove. 
C)  Topoisomerase II, which binds to the sugar-phosphate backbone. 
D)  SV40 large T-antigen, which binds to the major groove. 
E)  None of the above.

Exam 7: Recombinant Dna and Biotechnology

Which of the following is required for constructing a cDNA library?

Transgenic mouse lines are useful for studies of human disease for all the following reasons EXCEPT

Allele-specific oligonucleotide (ASO) analysis is used primarily for

Segments of pure samples of identical, relatively short DNA molecules from plasmids can be subdivided into smaller pieces by

Eukaryotic chromosomes are characterized by each of the following, EXCEPT

For an expression vector that will be used to produce mRNA in a prokaryotic system, which of the following is not required?

Which stage in the process of gene cloning are the restriction endonucleases utilized?

Southern blots are used to analyze DNA that has been fractionated based on difference in

The Holliday model of general recombination provides for

Recombinant DNA techniques currently can be used for all of the following except

Which of the following are not required for expression of a human cDNA cloned in

Reverse transcriptase carries out all of the following activities except?

It is sometimes possible to design a degenerate oligonucleotide probe for screening a DNA library if the amino acid sequence of the protein is available. These probes are

Reverse transcriptase catalyzes which of the following reactions?

For an expression vector that will be used to produce mRNA in a prokaryotic system, which of the following is not required?

A technique that is used to determine the abundance of a particular mRNA is

M17482Reverse transcriptase is employed in cDNA cloning as a

Attenuation is dependent upon all of the following EXCEPT

Which of the following enzymes can be used for the construction of a recombinant DNA molecule in vitro?

Accessibility of DNA to trans-acting molecules is, in general, reduced by its being packaged into a nucleosome core, both in vitro and in vivo. A prominent exception to this is the behavior of:

Eukaryotic Cell Structure

DNA and RNA: Composition and Structure

Protein I: Composition and Structure

DNA Replication, Recombination, and Repair

RNA: Transcription and Rna Processing

Protein Synthesis: Translation and Posttranslational Modifications

Regulation of Gene Expression

Protein II: Structure-Function Relationships in Protein Families

Enzymes: Classification, Kinetics, and Control

The Cytochromes P450 and Nitric Oxide Synthases

Biological Membranes: Structure and Membrane Transport

Fundamentals of Signal Transduction

Bioenergetics and Oxidative Metabolism

Carbohydrate Metabolism I: Major Metabolic Pathways and Their Control

Carbohydrate Metabolism II: Special Pathways and Glycoconjugates

Lipid Metabolism I: Synthesis, Storage, and Utilization of Fatty Acids and Triacylglycerols

Lipid Metabolism II: Pathways of Metabolism of Special Lipids

Amino Acid Metabolism

Purine and Pyrimidine Nucleotide Metabolism

Metabolic Interrelationships

Biochemistry of Hormones

Molecular Cell Biology

Cell Cycle, Programmed Cell Death, and Cancer

Digestion and Absorption of Basic Nutritional Constituents

Digestion and Absorption of Basic Nutritional Constituents

Principles of Nutrition I and II

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