Question 1

In the Holliday model for recombination, which is not true

Accepted Answer

A)  one of two recombination products are formed depending on how the branch is cleaved 
B)  strand exchange leads to an intermediate structure with crossed single strands 
C)  two homologous duplexes are aligned 
D)  the branch can move along the strands leading to additional strand exchange 
E)  the strand exchange is initiated by DNase I nicking. 
A)  one of two recombination products are formed depending on how the branch is cleaved 
B)  strand exchange leads to an intermediate structure with crossed single strands 
C)  two homologous duplexes are aligned 
D)  the branch can move along the strands leading to additional strand exchange 
E)  the strand exchange is initiated by DNase I nicking.

Question 2

Restriction enzymes have all of the following properties EXCEPT

Accepted Answer

A)  they cleave double stranded DNA. 
B)  they modify DNA by methylation. 
C)  they recognize specific DNA sequences. 
D)  they cleave DNA, producing staggered ends. 
A)  they cleave double stranded DNA. 
B)  they modify DNA by methylation. 
C)  they recognize specific DNA sequences. 
D)  they cleave DNA, producing staggered ends.

Question 3

Restriction endonucleases

Accepted Answer

A)  digest DNA duplex molecules from the 5'-OH ends. 
B)  attack only single stranded DNA. 
C)  have base sequence specificity. 
D)  randomly digest double stranded DNA molecules. 
E)  are not produced by bacteria. 
A)  digest DNA duplex molecules from the 5'-OH ends. 
B)  attack only single stranded DNA. 
C)  have base sequence specificity. 
D)  randomly digest double stranded DNA molecules. 
E)  are not produced by bacteria.

Question 4

Which of the following would be required for the efficient expression of human low density lipoprotein (LDL) in a bacterial host?

Accepted Answer

A)  a cDNA construct encoding LDL, under the control of a bacterial promoter 
B)  the human gene encoding LDL, under the control of a bacterial promoter 
C)  a cDNA construct encoding LDL, under the control of the LDL promoter 
D)  the human gene encoding LDL, under the control of the LDL promoter 
A)  a cDNA construct encoding LDL, under the control of a bacterial promoter 
B)  the human gene encoding LDL, under the control of a bacterial promoter 
C)  a cDNA construct encoding LDL, under the control of the LDL promoter 
D)  the human gene encoding LDL, under the control of the LDL promoter

Question 5

All of the following statements about genetic engineering are correct EXCEPT

Accepted Answer

A)  recipient strains of bacteria to be utilized for cloning of foreign DNA should have mutated restriction enzyme systems. 
B)  recombinant plasmids are introduced into bacterial cells by transformation. 
C)  recombinant DNA clones containing eukaryotic DNA inserts can be made to synthesize eukaryotic proteins in bacterial cells. 
D)  bacterial plasmid vectors replicate and lyse bacterial cells forming plaques. 
E)  DNA ligase is utilized to covalently join DNA molecules in the construction of recombinant molecules. 
A)  recipient strains of bacteria to be utilized for cloning of foreign DNA should have mutated restriction enzyme systems. 
B)  recombinant plasmids are introduced into bacterial cells by transformation. 
C)  recombinant DNA clones containing eukaryotic DNA inserts can be made to synthesize eukaryotic proteins in bacterial cells. 
D)  bacterial plasmid vectors replicate and lyse bacterial cells forming plaques. 
E)  DNA ligase is utilized to covalently join DNA molecules in the construction of recombinant molecules.

Question 6

Transgenic animals are defined as

Accepted Answer

A)  Those that have been treated with a drug regimen. 
B)  Those that are bred for polymorphic alleles of one gene. 
C)  Clones derived by nuclear transfer. 
D)  Those that carry a foreign gene that was deliberately inserted into their genomes. 
E)  Those that are infected with a virus to test vaccine efficacy. 
A)  Those that have been treated with a drug regimen. 
B)  Those that are bred for polymorphic alleles of one gene. 
C)  Clones derived by nuclear transfer. 
D)  Those that carry a foreign gene that was deliberately inserted into their genomes. 
E)  Those that are infected with a virus to test vaccine efficacy.

Question 7

Topoisomerase I

Accepted Answer

A)  unwinds double stranded DNA by breaking base pairs 
B)  will decrease the writhing number of negatively supercoiled DNA 
C)  will decrease the linking number of negatively supercoiled DNA 
D)  nicks one strand of double stranded DNA 
E)  cuts both strands of double stranded DNA 
A)  unwinds double stranded DNA by breaking base pairs 
B)  will decrease the writhing number of negatively supercoiled DNA 
C)  will decrease the linking number of negatively supercoiled DNA 
D)  nicks one strand of double stranded DNA 
E)  cuts both strands of double stranded DNA

Question 8

The severity of an inherited disease can depend on all of the following EXCEPT

Accepted Answer

A)  the presence of a closely linked RFLP marker. 
B)  the genetic background of the individual. 
C)  environmental influences. 
D)  the nature of the mutation. 
A)  the presence of a closely linked RFLP marker. 
B)  the genetic background of the individual. 
C)  environmental influences. 
D)  the nature of the mutation.

Question 9

All of the following are TRUE regarding restriction endonucleases EXCEPT

Accepted Answer

A)  they recognize specific palindromic sequences. 
B)  they may produce staggered ends when they cleave DNA into fragments. 
C)  they may produce flush ends when they cleave DNA into fragments. 
D)  they are used in gene cloning to cleave the single stranded regions of the cDNA produced by reverse transcriptase. 
E)  they are used to cleave plasmid DNA in specific places in gene cloning experiments. 
A)  they recognize specific palindromic sequences. 
B)  they may produce staggered ends when they cleave DNA into fragments. 
C)  they may produce flush ends when they cleave DNA into fragments. 
D)  they are used in gene cloning to cleave the single stranded regions of the cDNA produced by reverse transcriptase. 
E)  they are used to cleave plasmid DNA in specific places in gene cloning experiments.

Question 10

Which ONE of the following single stranded DNA molecules would be understood to contain a palindrome in the double stranded state as this term has been defined for DNA sequence arrangements?

Accepted Answer

A)  ApTpGpCpCpGpTpAp 
B)  GpCpTpApTpGpApCp 
C)  ApTpGpCpTpApCpGp 
D)  GpTpCpApTpGpApCp 
A)  ApTpGpCpCpGpTpAp 
B)  GpCpTpApTpGpApCp 
C)  ApTpGpCpTpApCpGp 
D)  GpTpCpApTpGpApCp

Question 11

Jumping clones expedite chromosomal walking by generating inserts that

Accepted Answer

A)  are compatible with YAC vectors. 
B)  contain non-contiguous but linearly related fragments. 
C)  can be amplified by the polymerase chain reaction. 
D)  contain unclonable sequences. 
A)  are compatible with YAC vectors. 
B)  contain non-contiguous but linearly related fragments. 
C)  can be amplified by the polymerase chain reaction. 
D)  contain unclonable sequences.

Question 12

The best technique for determining on which chromosome a specific human gene is located would probably involve

Accepted Answer

A)  digestion of total human DNA with restriction endonucleases, separation of fragments by gel electrophoresis and hybridization with a labeled gene probe. 
B)  somatic cell hybridization. 
C)  isolation of fetal messenger RNA and in vitro translation. 
D)  None of the above techniques could yield such information. 
A)  digestion of total human DNA with restriction endonucleases, separation of fragments by gel electrophoresis and hybridization with a labeled gene probe. 
B)  somatic cell hybridization. 
C)  isolation of fetal messenger RNA and in vitro translation. 
D)  None of the above techniques could yield such information.

Question 13

The Sanger method of nucleotide sequence determination involves

Accepted Answer

A)  the activity of DNA polymerase 
B)  the use of chemical agents that modify DNA 
C)  the transfer of electrophoretically resolved DNA to a nitrocellulose filter. 
D)  the incorporation of poly dT the 3'-OH end of a DNA strand. 
A)  the activity of DNA polymerase 
B)  the use of chemical agents that modify DNA 
C)  the transfer of electrophoretically resolved DNA to a nitrocellulose filter. 
D)  the incorporation of poly dT the 3'-OH end of a DNA strand.

Question 14

Polymerase chain reaction (PCR) results in discrete length products because

Accepted Answer

A)  Taq polymerase can only synthesize DNA of discrete size from any DNA template. 
B)  the reaction is terminated by denaturation at the same time following each round of polymerization. 
C)  the primers are of discrete size. 
D)  of the exponential production of run-off products. 
E)  the template for amplification is always a specific cloned DNA fragment. 
A)  Taq polymerase can only synthesize DNA of discrete size from any DNA template. 
B)  the reaction is terminated by denaturation at the same time following each round of polymerization. 
C)  the primers are of discrete size. 
D)  of the exponential production of run-off products. 
E)  the template for amplification is always a specific cloned DNA fragment.

Question 15

RNA-dependent-RNA-polymerases are required by all

Accepted Answer

A)  viruses. 
B)  non-tumor forming RNA viruses. 
C)  single stranded RNA viruses. 
D)  segmented-genome viruses. 
A)  viruses. 
B)  non-tumor forming RNA viruses. 
C)  single stranded RNA viruses. 
D)  segmented-genome viruses.

Question 16

In general, PCR primers need to have which of the following characteristics?

Accepted Answer

A)  Each primer needs to base-pair with a site on one of the two DNA strands. 
B)  The primers need to base-pair with each other. 
C)  Each primer needs to base-pair with two sites on each of the two DNA strands. 
D)  The two primers need to base-pair with sites that are repetitive in the genome. 
A)  Each primer needs to base-pair with a site on one of the two DNA strands. 
B)  The primers need to base-pair with each other. 
C)  Each primer needs to base-pair with two sites on each of the two DNA strands. 
D)  The two primers need to base-pair with sites that are repetitive in the genome.

Question 17

All of the following statements concerning DNA cloning are true EXCEPT which one?

Accepted Answer

A)  Vectors such as plasmids or bacterial viruses can be used for cloning foreign DNA in bacterial cells. 
B)  Recombinant molecules between plasmid DNA and foreign DNA can be generated by using restriction endonucleases. 
C)  cDNA synthesized from the messenger RNA of a desired gene can be cloned. 
D)  If foreign DNA is inserted into a bacterial plasmid, the recombinant plasmid cannot be used for transforming eukaryotic cells. 
E)  Some eukaryotic genes can be expressed when transformed into bacteria. 
A)  Vectors such as plasmids or bacterial viruses can be used for cloning foreign DNA in bacterial cells. 
B)  Recombinant molecules between plasmid DNA and foreign DNA can be generated by using restriction endonucleases. 
C)  cDNA synthesized from the messenger RNA of a desired gene can be cloned. 
D)  If foreign DNA is inserted into a bacterial plasmid, the recombinant plasmid cannot be used for transforming eukaryotic cells. 
E)  Some eukaryotic genes can be expressed when transformed into bacteria.

Question 18

Hybridization (Southern) analysis is best used to

Accepted Answer

A)  compare polypeptide conformation. 
B)  prepare large, unilamellar lipid vesicles. 
C)  determine enzyme turnover numbers. 
D)  establish DNA sequence homologies. 
E)  investigate ribosome assembly. 
A)  compare polypeptide conformation. 
B)  prepare large, unilamellar lipid vesicles. 
C)  determine enzyme turnover numbers. 
D)  establish DNA sequence homologies. 
E)  investigate ribosome assembly.

Exam 7: Recombinant Dna and Biotechnology

In the Holliday model for recombination, which is not true

Restriction enzymes have all of the following properties EXCEPT

Restriction endonucleases

Which of the following would be required for the efficient expression of human low density lipoprotein (LDL) in a bacterial host?

All of the following statements about genetic engineering are correct EXCEPT

Transgenic animals are defined as

Topoisomerase I

The severity of an inherited disease can depend on all of the following EXCEPT

All of the following are TRUE regarding restriction endonucleases EXCEPT

Which ONE of the following single stranded DNA molecules would be understood to contain a palindrome in the double stranded state as this term has been defined for DNA sequence arrangements?

Jumping clones expedite chromosomal walking by generating inserts that

The best technique for determining on which chromosome a specific human gene is located would probably involve

The Sanger method of nucleotide sequence determination involves

Polymerase chain reaction (PCR) results in discrete length products because

RNA-dependent-RNA-polymerases are required by all

In general, PCR primers need to have which of the following characteristics?

All of the following statements concerning DNA cloning are true EXCEPT which one?

Hybridization (Southern) analysis is best used to

Eukaryotic Cell Structure

DNA and RNA: Composition and Structure

Protein I: Composition and Structure

DNA Replication, Recombination, and Repair

RNA: Transcription and Rna Processing

Protein Synthesis: Translation and Posttranslational Modifications

Regulation of Gene Expression

Protein II: Structure-Function Relationships in Protein Families

Enzymes: Classification, Kinetics, and Control

The Cytochromes P450 and Nitric Oxide Synthases

Biological Membranes: Structure and Membrane Transport

Fundamentals of Signal Transduction

Bioenergetics and Oxidative Metabolism

Carbohydrate Metabolism I: Major Metabolic Pathways and Their Control

Carbohydrate Metabolism II: Special Pathways and Glycoconjugates

Lipid Metabolism I: Synthesis, Storage, and Utilization of Fatty Acids and Triacylglycerols

Lipid Metabolism II: Pathways of Metabolism of Special Lipids

Amino Acid Metabolism

Purine and Pyrimidine Nucleotide Metabolism

Metabolic Interrelationships

Biochemistry of Hormones

Molecular Cell Biology

Cell Cycle, Programmed Cell Death, and Cancer

Digestion and Absorption of Basic Nutritional Constituents

Digestion and Absorption of Basic Nutritional Constituents

Principles of Nutrition I and II

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