Question 1

Applications using transgenic mice include all of the following EXCEPT

Accepted Answer

A)  rescue of mutants. 
B)  generation of chemically_induced mutant loci. 
C)  synthetic bioreactors. 
D)  testing drug or gene therapies. 
E)  studies of structure_function relationships. 
A)  rescue of mutants. 
B)  generation of chemically_induced mutant loci. 
C)  synthetic bioreactors. 
D)  testing drug or gene therapies. 
E)  studies of structure_function relationships.

Question 2

M1749Which of the following is not always required for DNA blotting (Southern hybridization)?

Accepted Answer

A)  digestion of DNA with restriction endonucleases 
B)  separation of digested DNA by electrophoresis 
C)  amplification of DNA fragment by polymerase chain reaction (PCR) 
D)  hybridization with some kind of probe which contains regions of complementarity 
A)  digestion of DNA with restriction endonucleases 
B)  separation of digested DNA by electrophoresis 
C)  amplification of DNA fragment by polymerase chain reaction (PCR) 
D)  hybridization with some kind of probe which contains regions of complementarity

Question 3

A nuclease is an enzyme which catalyzes

Accepted Answer

A)  the synthesis of nucleotides from bases and sugar-phosphates. 
B)  the hydrolysis of nucleotides to nucleosides and inorganic phosphate. 
C)  the hydrolysis of cyclic AMP. 
D)  the synthesis of a polynucleotide chain from nucleotide units. 
E)  the hydrolysis of internucleotide linkages of a polynucleotide chain. 
A)  the synthesis of nucleotides from bases and sugar-phosphates. 
B)  the hydrolysis of nucleotides to nucleosides and inorganic phosphate. 
C)  the hydrolysis of cyclic AMP. 
D)  the synthesis of a polynucleotide chain from nucleotide units. 
E)  the hydrolysis of internucleotide linkages of a polynucleotide chain.

Question 4

Selection of a Yeast Artificial Chromosome (YAC) vector for the cloning of human genomic DNA would greatly expedite

Accepted Answer

A)  chromosomal walking. 
B)  efficient translation of human proteins. 
C)  expression screening. 
D)  differential screening. 
A)  chromosomal walking. 
B)  efficient translation of human proteins. 
C)  expression screening. 
D)  differential screening.

Question 5

RFLP linkage analysis followed by chromosome walking is used to find disease-causing genes. Which of the following would be a good indication that the disease-causing gene has been found by this analysis?

Accepted Answer

A)  The gene is at least one cM from the RFLP. 
B)  The gene is homozygous in the affected individual. 
C)  The gene contains a nonsense mutation. 
D)  The gene is heterozygous in the individual. 
E)  The gene contains numerous polymorphisms. 
A)  The gene is at least one cM from the RFLP. 
B)  The gene is homozygous in the affected individual. 
C)  The gene contains a nonsense mutation. 
D)  The gene is heterozygous in the individual. 
E)  The gene contains numerous polymorphisms.

Question 6

Eukaryotic genes coding for proteins usually have introns. The production of recombinant eukaryotic proteins (insulin, for example) in bacteria does not necessarily pose a problem because

Accepted Answer

A)  bacteria possess the necessary enzymes to correctly process the primary transcript containing intron sequences. 
B)  a cDNA copy of the mRNA can be cloned and expressed. 
C)  the protein synthetic machinery of the bacteria will only translate the exon portions of the resulting RNA. 
D)  the translation of intron regions will not alter the activity of the protein if they are removed by proteolytic processing. 
A)  bacteria possess the necessary enzymes to correctly process the primary transcript containing intron sequences. 
B)  a cDNA copy of the mRNA can be cloned and expressed. 
C)  the protein synthetic machinery of the bacteria will only translate the exon portions of the resulting RNA. 
D)  the translation of intron regions will not alter the activity of the protein if they are removed by proteolytic processing.

Question 7

Human genomic libraries would be expected to contain all of the following EXCEPT

Accepted Answer

A)  introns. 
B)  exons. 
C)  promoters. 
D)  spliced sequences. 
E)  Alu sequences. 
A)  introns. 
B)  exons. 
C)  promoters. 
D)  spliced sequences. 
E)  Alu sequences.

Question 8

DNA libraries contain

Accepted Answer

A)  copies of genes. 
B)  mRNAs. 
C)  fragments of genomic DNA. 
D)  DNA copies of mRNA. 
E)  intron DNA sequences. 
A)  copies of genes. 
B)  mRNAs. 
C)  fragments of genomic DNA. 
D)  DNA copies of mRNA. 
E)  intron DNA sequences.

Question 9

In constructing a genomic DNA library, limited digestion with a restriction enzyme that recognizes and cuts a 4 base pair sequence

Accepted Answer

A)  ensures that the DNA fragments being cloned are very small. 
B)  ensures that introns are separated from exons. 
C)  generates overlapping fragments of the genome. 
D)  separates repetitive sequences from unique sequences. 
A)  ensures that the DNA fragments being cloned are very small. 
B)  ensures that introns are separated from exons. 
C)  generates overlapping fragments of the genome. 
D)  separates repetitive sequences from unique sequences.

Question 10

Restriction enzymes have all of the following characteristics EXCEPT that they cut

Accepted Answer

A)  DNA sequence specifically. 
B)  each DNA strand separately. 
C)  palindromic sequences. 
D)  DNA using an exonuclease activity. 
A)  DNA sequence specifically. 
B)  each DNA strand separately. 
C)  palindromic sequences. 
D)  DNA using an exonuclease activity.

Question 11

Reverse transcription refers to

Accepted Answer

A)  DNA synthesis from an RNA template. 
B)  RNA synthesis from an RNA template. 
C)  protein synthesis. 
D)  movement of ribosomes along a messenger RNA. 
E)  modification of nucleic acid bases. 
A)  DNA synthesis from an RNA template. 
B)  RNA synthesis from an RNA template. 
C)  protein synthesis. 
D)  movement of ribosomes along a messenger RNA. 
E)  modification of nucleic acid bases.

Question 12

Southern blotting is used for all of the following, EXCEPT

Accepted Answer

A)  to determine restriction fragment sizes. 
B)  to detect deleted genes. 
C)  to detect gene rearrangements. 
D)  to measure mRNA size. 
A)  to determine restriction fragment sizes. 
B)  to detect deleted genes. 
C)  to detect gene rearrangements. 
D)  to measure mRNA size.

Question 13

Which of the following statements about homologous recombination is TRUE?

Accepted Answer

A)  recombination can only occur at Chi sites 
B)  recombination only occurs at specific DNA sequences 
C)  recombination requires a single_stranded region and a duplex DNA region with closely related DNA sequence 
D)  recombination requires an RNA intermediate 
E)  recombination is site-specific 
A)  recombination can only occur at Chi sites 
B)  recombination only occurs at specific DNA sequences 
C)  recombination requires a single_stranded region and a duplex DNA region with closely related DNA sequence 
D)  recombination requires an RNA intermediate 
E)  recombination is site-specific

Question 14

Bacteria were infected with phages labeled with 35S and then whirled in a food blender. Cells were then separated from the fluid medium supernatant. Which of the following would be true?

Accepted Answer

A)  No radioactivity would be detected in either cells or supernatant. 
B)  The radioactivity would be found in the progeny phage particles if infection of the cells was allowed to continue. 
C)  The supernatant fluid would contain most of the radioactivity 
D)  The radioactivity would be found in the phage DNA within the infected cells 
A)  No radioactivity would be detected in either cells or supernatant. 
B)  The radioactivity would be found in the progeny phage particles if infection of the cells was allowed to continue. 
C)  The supernatant fluid would contain most of the radioactivity 
D)  The radioactivity would be found in the phage DNA within the infected cells

Question 15

Which one of the following statements about Southern blot analysis is true?

Accepted Answer

A)  It will identify which RNA in a population is complementary to a specific DNA probe. 
B)  It is used to purify a gene by electrophoretic separation from a population of genes. 
C)  It is useful for repetitive DNA but not unique sequence DNA. 
D)  It is used to amplify a specific DNA sequence in vitro. 
E)  It identifies a specific fragment of DNA based on complementarity to a radioactive DNA probe. 
A)  It will identify which RNA in a population is complementary to a specific DNA probe. 
B)  It is used to purify a gene by electrophoretic separation from a population of genes. 
C)  It is useful for repetitive DNA but not unique sequence DNA. 
D)  It is used to amplify a specific DNA sequence in vitro. 
E)  It identifies a specific fragment of DNA based on complementarity to a radioactive DNA probe.

Question 16

In the dideoxy method of DNA sequencing, the dideoxy analog

Accepted Answer

A)  allows specific chemical cleavage of the DNA strand at random sites. 
B)  is required for the synthesis of the primer. 
C)  causes termination of DNA chain elongation. 
D)  cannot be utilized by DNA polymerase. 
E)  lacks a 5' phosphate group. 
A)  allows specific chemical cleavage of the DNA strand at random sites. 
B)  is required for the synthesis of the primer. 
C)  causes termination of DNA chain elongation. 
D)  cannot be utilized by DNA polymerase. 
E)  lacks a 5' phosphate group.

Question 17

DNA libraries are constructed from

Accepted Answer

A)  genomic DNA from specific tissues. 
B)  reverse transcribed cellular RNA. 
C)  reverse translated cellular proteins. 
D)  reverse transcriptase. 
E)  satellite DNA. 
A)  genomic DNA from specific tissues. 
B)  reverse transcribed cellular RNA. 
C)  reverse translated cellular proteins. 
D)  reverse transcriptase. 
E)  satellite DNA.

Question 18

Isolation and identification of a disease-causing gene by chromosome walking requires all of the following EXCEPT

Accepted Answer

A)  Genomic library 
B)  DNA sequence analysis 
C)  Comparison of the normal and mutant genes 
D)  Prior knowledge of the protein product 
E)  Overlapping genomic clones 
A)  Genomic library 
B)  DNA sequence analysis 
C)  Comparison of the normal and mutant genes 
D)  Prior knowledge of the protein product 
E)  Overlapping genomic clones

Question 19

Attenuation is dependent upon all of the following EXCEPT:

Accepted Answer

A)  direct physical interaction between the ribosome and RNA polymerase 
B)  pausing of a ribosome in the leader peptide due to low levels of an operon specific charged tRNA 
C)  no proteins other than those in ribosomes and RNA polymerase 
D)  the formation of a stem loop structure in the nascent mRNA strand that acts as a transcriptional terminator 
A)  direct physical interaction between the ribosome and RNA polymerase 
B)  pausing of a ribosome in the leader peptide due to low levels of an operon specific charged tRNA 
C)  no proteins other than those in ribosomes and RNA polymerase 
D)  the formation of a stem loop structure in the nascent mRNA strand that acts as a transcriptional terminator

Question 20

Reverse transcriptase has all of the following characteristics EXCEPT that it

Accepted Answer

A)  is a viral DNA polymerase. 
B)  requires a primer. 
C)  uses either DNA or RNA as a template. 
D)  synthesizes both DNA and RNA. 
A)  is a viral DNA polymerase. 
B)  requires a primer. 
C)  uses either DNA or RNA as a template. 
D)  synthesizes both DNA and RNA.

Exam 7: Recombinant Dna and Biotechnology

Applications using transgenic mice include all of the following EXCEPT

M1749Which of the following is not always required for DNA blotting (Southern hybridization)?

A nuclease is an enzyme which catalyzes

Selection of a Yeast Artificial Chromosome (YAC) vector for the cloning of human genomic DNA would greatly expedite

RFLP linkage analysis followed by chromosome walking is used to find disease-causing genes. Which of the following would be a good indication that the disease-causing gene has been found by this analysis?

Eukaryotic genes coding for proteins usually have introns. The production of recombinant eukaryotic proteins (insulin, for example) in bacteria does not necessarily pose a problem because

Human genomic libraries would be expected to contain all of the following EXCEPT

DNA libraries contain

In constructing a genomic DNA library, limited digestion with a restriction enzyme that recognizes and cuts a 4 base pair sequence

Restriction enzymes have all of the following characteristics EXCEPT that they cut

Reverse transcription refers to

Southern blotting is used for all of the following, EXCEPT

Which of the following statements about homologous recombination is TRUE?

Bacteria were infected with phages labeled with 35S and then whirled in a food blender. Cells were then separated from the fluid medium supernatant. Which of the following would be true?

Which one of the following statements about Southern blot analysis is true?

In the dideoxy method of DNA sequencing, the dideoxy analog

DNA libraries are constructed from

Isolation and identification of a disease-causing gene by chromosome walking requires all of the following EXCEPT

Attenuation is dependent upon all of the following EXCEPT:

Reverse transcriptase has all of the following characteristics EXCEPT that it

Eukaryotic Cell Structure

DNA and RNA: Composition and Structure

Protein I: Composition and Structure

DNA Replication, Recombination, and Repair

RNA: Transcription and Rna Processing

Protein Synthesis: Translation and Posttranslational Modifications

Regulation of Gene Expression

Protein II: Structure-Function Relationships in Protein Families

Enzymes: Classification, Kinetics, and Control

The Cytochromes P450 and Nitric Oxide Synthases

Biological Membranes: Structure and Membrane Transport

Fundamentals of Signal Transduction

Bioenergetics and Oxidative Metabolism

Carbohydrate Metabolism I: Major Metabolic Pathways and Their Control

Carbohydrate Metabolism II: Special Pathways and Glycoconjugates

Lipid Metabolism I: Synthesis, Storage, and Utilization of Fatty Acids and Triacylglycerols

Lipid Metabolism II: Pathways of Metabolism of Special Lipids

Amino Acid Metabolism

Purine and Pyrimidine Nucleotide Metabolism

Metabolic Interrelationships

Biochemistry of Hormones

Molecular Cell Biology

Cell Cycle, Programmed Cell Death, and Cancer

Digestion and Absorption of Basic Nutritional Constituents

Digestion and Absorption of Basic Nutritional Constituents

Principles of Nutrition I and II

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