Exam 5: Introduction to Proteins: the Primary Level of Protein Structure

Several homologous proteins can be aligned to provide a ________ sequence.

When referring to the amino acid sequences of proteins, sequence homology is the same as sequence similarity.

Which of the following statements about insulin is INCORRECT?

Which of the following modified amino acids is incorporated during translation rather than being modified post-translationally?

The chemical ________ can cleave amide bonds on the C-terminal side of methionine residues.

At pH=0, the net charge on a polypeptide will be negative.

Applications of mass spectrometry include:

Most proteins have blocked amino and carboxyl terminals.

Which of the following amino acids would most likely be found on the surface of a protein?

Recombinant proteins containing a ________ can be purified using immobilized metal affinity chromatography and eluted by the addition of imidazole, a low pH buffer or a buffer containing EDTA.

An amide bond between the α-carboxylic acid group of one amino acid and the α-amino group on another is called a ________.

Protein biosynthesis uses only L-amino acids.

Which of the following is FALSE when considering the standard genetic code?

All amino acids have a chiral α-carbon EXCEPT ________.

The side chain of ________ has a pKa in the physiological pH range and is therefore often involved in proton transfer during enzymatic catalysis.

In size exclusion chromatography, the smallest proteins are eluted last.

________ chromatography is used to separate proteins based on their surface charge.

At physiological pH, the carboxylic acid group of an amino acid will be ________, while the amino group will be ________, yielding the zwitterion form.

Conservative amino acid changes never affect stability or function of a protein.