Exam 20: Dna Tools and Biotechnology

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with a recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes (endonucleases). III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA.

Which of the following statements correctly describes how RNA interference (RNAi) silences selected genes?

Which of the following processes helps bacterial cells protect their own DNA from restriction enzymes (endonucleases)?

In an experiment, DNA from the linear form of the bacteriophage Lambda was cut into fragments using the restriction enzyme Hind III. Hind III cuts the Lambda DNA between the adenine nucleotides on the complementary strands in a specific sequence, as indicated in the diagram, producing eight different size fragments. These fragments are then separated with an electrical current based on size after the DNA fragments are placed in a porous gel, a process called gel electrophoresis. Select an observation that best describes a correct aspect of the two processes of restriction Digest and gel electrophoresis.

Which of the following enzymes is required to make complementary DNA (cDNA) from RNA?

A principal problem with inserting an unmodified mammalian gene into a plasmid and then getting that gene expressed in bacteria is that ________.

Which of the following characteristics of short tandem repeats (STRs) DNA makes it useful for DNA fingerprinting?

For a particular microarray assay (DNA chip), cDNA has been made from the mRNAs of a dozen patients' breast tumour biopsies. Which of the following types of evidence will researchers be looking for in order to determine if the cells are cancerous?

Which of the following tools of DNA technology is incorrectly paired with its use?

Which of the following correctly lists the processes in order for one cycle of polymerase chain reaction (PCR)?

For which of the following processes can dideoxyribonucleotides be used?

Which of the following characteristics of Taq polymerase make it useful in the PCR process?

How have DNA microarrays made a huge impact on genomic studies?

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of

One possible use of transgenic plants is in the production of human proteins, such as vaccines. Which of the following issues is a possible hindrance that must be overcome in order for this process to work properly?

Transgenic mice are useful to human researchers because they ________.

Which of the following statements correctly describes one of the main differences between embryonic stem cells and adult stem cells?

The segment of DNA shown in the figure has restriction sites I and II, which create restriction fragments A, B and C. Which of the gels produced by electrophoresis best represents the separation and identity of these fragments?

Which of the following sequences is most likely to be cut by a restriction enzyme?

Reproductive cloning of human embryos is generally considered unethical. However, on the subject of therapeutic cloning, there is a wider divergence of opinion. Which of the following statements provides a likely explanation?